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Full-Text Articles in Life Sciences
Reef Fish Biodiversity In The Florida Keys National Marine Sanctuary, Megan E. Hepner
Reef Fish Biodiversity In The Florida Keys National Marine Sanctuary, Megan E. Hepner
USF Tampa Graduate Theses and Dissertations
The biological diversity of reef-fish in the Florida Keys National Marine Sanctuary (FKNMS) from 1999 – 2016 was evaluated in terms of abundance, biomass, species richness, evenness, Shannon diversity, Simpson diversity, and functional diversity, using observations collected by multiple agencies and institutions under the Reef Visual Census (RVC) program. To compare the different diversity indices species richness, Shannon diversity, Simpson diversity, and functional diversity were converted into effective number of species. I examined the seven indices by no-take marine zones, in seven benthic habitat strata, and across the three-distinct geographic subregions in the Florida Keys domain (Upper, Middle, and Lower …
Evaluation Of Filtration And Dna Extraction Methods For Environmental Dna Biodiversity Assessments Across Multiple Trophic Levels, Anni Djurhuus, Jesse Port, Collin Closek, Kevan Yamahara, Ofelia Romero-Maraccini, Kristine Walz, Dawn Goldsmith, Reiko Michisaki, Mya Breitbart, Alexandria Boehm, Francisco Chavez
Evaluation Of Filtration And Dna Extraction Methods For Environmental Dna Biodiversity Assessments Across Multiple Trophic Levels, Anni Djurhuus, Jesse Port, Collin Closek, Kevan Yamahara, Ofelia Romero-Maraccini, Kristine Walz, Dawn Goldsmith, Reiko Michisaki, Mya Breitbart, Alexandria Boehm, Francisco Chavez
Marine Science Faculty Publications
Metabarcoding of marine environmental DNA (eDNA), originating from tissue, cells, or extracellular DNA, offers the opportunity to survey the biological composition of communities across multiple trophic levels from a non-invasive seawater sample. Here we compare results of eDNA metabarcoding of multiple trophic levels from individual seawater samples collected from a kelp forest in Monterey Bay, California in order to establish methods for future cross-trophic level eDNA analysis. Triplicate 1 L water samples were filtered using five different 47 mm diameter membrane filters (PVDF, PES, GFF, PCTE, and NC) and DNA was extracted from triplicates of each filter-type using three widely-used …