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Life Sciences Commons

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University of South Florida

Theses/Dissertations

2020

EDNA

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Full-Text Articles in Life Sciences

Succession In Native Groundwater Microbial Communities In Response To Effluent Wastewater, Chelsea M. Dinon Oct 2020

Succession In Native Groundwater Microbial Communities In Response To Effluent Wastewater, Chelsea M. Dinon

USF Tampa Graduate Theses and Dissertations

Aquifer storage and recovery techniques are used globally to mitigate increasing demands for groundwater. Microcosms were used to evaluate the effect of wastewater effluent that may be used for aquifer storage and recovery injection on native aquifer microbial communities. Sulfur Springs water was used as the source of aquifer water. The microcosms were 100% spring water, 100% unchlorinated filtered wastewater effluent, or one of three mixtures of unchlorinated filtered wastewater effluent and spring water. The mixture microcosms were 50%, 30%, and 10% unchlorinated filtered wastewater effluent with the balance as spring water. The effluent water was UV treated prior to …


Spatiotemporal Changes Of Microbial Community Assemblages And Functions In The Subsurface, Madison C. Davis Mar 2020

Spatiotemporal Changes Of Microbial Community Assemblages And Functions In The Subsurface, Madison C. Davis

USF Tampa Graduate Theses and Dissertations

The subsurface hosts diverse microbial community assemblages and functions. These communities play an important role in biogeochemical cycling and groundwater purification. Many physicochemical factors affect microbial communities and can cause short-term or long-term perturbations. Subsurface microbes are susceptible to anthropogenic changes in the environment, which can be caused by nutrient inputs or municipal groundwater extraction. Despite the importance of the subsurface microbiome, these microbial communities are poorly characterized. This dissertation describes the characterization of spatiotemporal drivers of subsurface microbial communities through a variety of techniques that include eDNA analyses, bioinformatics, hydrochemical analyses, stable isotope geochemistry, and multivariate statistics. Three coastal …