Life Sciences Commons^™

Doc Dynamics In Eddies Of The Sargasso Sea, Eric Matthew Lachenmyer

Theses and Dissertations

Bacterial productivity and size-fractioned rates of dissolved organic carbon (DOC) excretion from primary and secondary producers were measured in two eddy types, one anti-cyclonic (February 2012) and one cyclonic eddy (July 2012), in the Sargasso Sea. Bacterial productivity (BP) rates in the cyclonic eddy were highest in the center (9.2 mg C m-2d-1) and edge (10.4 mg C m-2d-1) of the eddy compared to the anti-cyclone center (2.2 mg C m-2d-1) and edge (5.1 mg C m-2d-1). Rates of DOC excretion from14C-tracer experiments were not significantly higher than background; lack of accumulation of labeled material indicated very fast uptake of …

Nutrient Limitation Of Bioluminescent Dinoflagellates In Mangrove Lagoon, Salt River Bay, St. Croix, Usvi, Michelle Zimberlin

Theses and Dissertations

Bioluminescent bays resulting from dense concentrations of bioluminescent dinoflagellates are rare, with only 14 documented systems worldwide. One of these bays, Mangrove Lagoon, is located in Salt River Bay National Park in St. Croix, USVI. Very little is known about the environmental factors responsible for maintaining the high dinoflagellate densities in this lagoon. In order to assess the dynamics of the dinoflagellates, in situ nutrient addition bioassays were conducted to determine which nutrients regulate the phytoplankton community, which includes the bioluminescent dinoflagellate Pyrodinium bahamense. Bioassays were conducted in two different seasons, (January 2013 and May 2013) to examine seasonal responses …

Characterization Of The Role Of Fro6 In Metal Homeostasis In Arabidopsis Thaliana, Margo Ellen Maynes

Theses and Dissertations

Iron deficiency is the most common human nutritional disorder in the world today, affecting approximately 2 billon people worldwide, almost a third of the world's population. For most of the world's population, plant foods serve as the major source of dietary iron. One approach that may contribute towards the solution of iron deficiency is the development of crop plants that contain higher levels of bioavailable iron. This project focuses on FRO6, a ferric chelate reductase in Arabidopsis thaliana that is thought to reduce apoplastic iron for transport across the plasma membrane in leaf cells. We hypothesize therefore that FRO6 controls …

The Role Of Host-Tumor Interactions In Liver Metastasis Of Colorectal Cancer, Yu Zhang

Theses and Dissertations

Colon cancer is the third most frequent cancer and the second leading cause of cancer deaths in the United States. Liver metastasis is the major cause of death in colon cancer. Successful metastases depend on productive collaborations between tumor cells and host-derived cells in the tumor microenvironment, target organ environments, and cells in the hematopoietic compartment.

To identify the host-tumor interactions promoting liver metastasis and their molecular and cellular mediators, an orthotopic mouse model of liver metastasis of colon cancer was established that recapitulates all stages of tumor growth and metastasis. A highly metastatic mouse carcinoma cell line CT26-FL3 was …

The Effect Of Cancer Cachexia Severity And Eccentric Muscle Contractions On Selected Myofiber Metabolic Properties In Mouse Skeletal Muscle, Joshua Earl Mangum

Theses and Dissertations

Cancer cachexia is a complex syndrome that induces skeletal muscle wasting and dysregulation of skeletal muscle metabolism. Alterations in area of myofibers and myofiber metabolic properties can significantly impact the function of skeletal muscle. The purpose of this study was to examine the effect of cachexia severity and resistance exercise training on frequency and area of selected myofiber metabolic characteristics during cachexia-induced myofiber atrophy. Male ApcMin/+ (Min) mice were studied during the progression of cachexia (16-20 weeks of age) and stratified into groups based on the severity of cachexia. For the second study, male Min mice performed resistance exercise (RE) …

Tidal Variability Of Waves And Currents On A Caribbean Barrier Reef, Chelsea Wegner

Theses and Dissertations

The modern framework of Caribbean coral reefs is a product of resilient structures that have survived extreme variations in sea level for the past 18,000 years. However, the added influences of anthropogenic pressures, including ocean acidification, overfishing, pollution and bleaching make the future response to sea level rise uncertain. Carbonate production in the Caribbean is no longer at these historic rates and reefs may not be able to keep pace with the projected increases in sea level. This could have dramatic impacts on the hydrodynamics in coral reef environments, as reef morphology strongly influences these processes. Coral reefs are regarded …

Executing And Teaching Science--The Breast Cancer Genetics And Technology-Rich Curriculum Professional Development Studies Of A Science Educator, Regina Evarn Wragg

Theses and Dissertations

This dissertation presents my explorations in both molecular biology and science education research. In study one, we determined the ADIPOQ and ADIPORI genotypes of 364 White and 148 Black BrCa patients and used dominant model univariate logistic regression analyses to determine individual SNP and haplotype associations with tumor or patient characteristics in a case-case comparison. We found twelve associations between individual SNPs and patient or tumor characteristics that impact BrCa prognosis. For example, the ADIPOQ rs1501299 C allele was associated with ER+ tumors (OR=4.73, p=0.001) among White women >50 years of age at their time of diagnosis. Also, the A …

Differential Gene Expression Patterns In Hpv-Positive And Hpv-Negative Oropharyngeal Carcinomas, Swati Tomar

Theses and Dissertations

Head and neck (HN) cancer is the fifth most prevalent malignancy worldwide with 90% of them categorized as squamous cell carcinomas (SCC). Carcinomas of the oropharynx, pharynx and the oral cavity, comprise a subset of HNSCC and are referred to as oropharyngeal squamous cell carcinomas (OPSCC). Up to 60% of OPSCC and 25% of HNSCC are positive for high-risk human papillomavirus (HR-HPV), primarily HPV16. HPV positive and HPV negative OPSCC’s are molecularly and biologically distinct with differences in risk factors, age of presentation and clinical behavior. The precise molecular signatures of each have been well studied with respect to gene …

A Critical Review Of Endometriosis Pathology, Brittany Lauren Cureton

Theses and Dissertations

Endometriosis is a complex, multifactorial, reproductive disorder present in approximately 10-15% of adult women between the ages of 25-35. This disorder occurs when endometrial glands and stroma grow ectopically on the surface of the ovaries, pelvic peritoneum, fallopian tubes, and the uterus. Endometriosis causes varying degrees of painful symptoms and infertility in infected individuals. Three main theories of endometrial accumulation attempt to explain the etiology of this elusive disease. There have been various staging of endometriosis symptoms that attempt to standardize classification as well as predict pain and infertility. Angiogenesis, necessary to the survival of endometrial tissue, along with immune …

Associations Between Resting Metabolic Rate And Energy Storage In Young Adults, Robin Shook

Theses and Dissertations

At the most basic level obesity is the result of a chronic imbalance between energy intake and energy expenditure. However, the exact etiology is considerably more complex and may involve a variety of physiological and behavioral factors. Metabolic disturbances, including reduced fat oxidation as measured by the respiratory quotient (RQ) and reduced resting metabolic rate (RMR), have been identified as possible predictors of changes in body weight and body composition. RMR represents the largest component (60-80%) of caloric expenditure that contributes to total daily energy expenditure in humans and has high inter-person variability (±25%) but not within individuals (<±5%). The cause of this variability between individuals and the exact role of RMR and RQ in determining body weight and body composition are uncertain.

This …

The Mechanisms Of Posterior Shoulder Tightness And Effectiveness Of Manual Therapy, Lane Brooks Bailey

Theses and Dissertations

Posterior shoulder tightness is a common physical impairment in overhand baseball athletes presenting with injury. The etiology of this physical impairment is poorly understood and theorized to be a combination of bony, muscular, and inert soft-tissue contributions occurring at the glenohumeral joint. The ability to discriminate between each tissue's influences on shoulder range of motion is often challenging to overcome within a clinical environment. Chapter 2 of this manuscript provides a thorough review of the literature discussing the potential mechanisms of posterior shoulder tightness. Previous studies have independently accounted for the relationships between posterior shoulder tightness, and the mechanical contributions …

Decision-Support Tool For Residential Pesticides In The South Carolina Coastal Zone, Lisa Claire Wickliffe

Theses and Dissertations

The Environmental Protection Agency (EPA) is charged with ensuring pesticides do not pose unreasonable adverse risks to the public and to the environment. This is a daunting task with over one billion pounds of pesticides used across the nation each year. The U.S. EPA estimates approximately 75% of all pesticide usage in the U.S. are agricultural while 25% is for home, garden, industrial, commercial, and government applications. One area of application of concern to public health and the environment regarding misuse of pesticides is in residential settings. In these instances, individuals may not have any knowledge of identifying whether they …

The Effects Of A High Fat Diet On Mitochondrial Biogenesis And Inflammation In The Brain., Lauren Suzanne Vervaecke

Theses and Dissertations

INTRO: High fat diets (HFD) ingested over long periods of time have been shown to cause diet induced obesity leading to excessive adiposity and chronic low grade inflammation. Here we have shown how differing levels of saturated fats (SF), which are common storage fats, affect markers of inflammation and mitochondrial biogenesis. IL-1â and TNF-alpha are pro-inflammatory cytokines that have been shown to increase with a HFD, while molecular signalers, PGC-1alpha, cyt c, and SIRT1, related to mitochondrial biogenesis have been shown to decrease. Despite the risks of consuming a diet high in SF's, consumers continue to buy energy dense foods …

An Investigation Into The Influence Of Dietary Saturated Fat And Quercetin Supplementation On Adiposity, Macrophage Behavior, Inflammation, And Non-Alcoholic Fatty-Liver Disease, Reilly Enos

Theses and Dissertations

The overall objective of this dissertation was to examine the degree to which manipulation of the saturated fat content of a high-fat diet influenced obesity-related outcomes as well as to see if the naturally-occurring flavonoid, quercetin, could attenuate the resulting obesity and related metabolic and inflammatory side effects. Specifically, these studies examined 1) the influential role of saturated fat on macrophage function, inflammation, and other obesity-related comorbidities, including non-alcoholic fatty-liver disease, and 2) the potential benefits of quercetin supplementation. Overall, results from these studies suggest that adiposity, macrophage behavior, inflammation, insulin resistance, and non-alcoholic fatty-liver disease progression can be greatly …

Measurement And Three-Dimensional Modeling Of Hydrodynamic Processes In The Inner Shelf And Surf Zone, Nirnimesh Kumar

Theses and Dissertations

Wind stress and surface gravity waves play an important role in creating and modifying flows on the shelf. The importance of surface waves increases in shallow waters especially within the inner shelf and the surf zone. Understanding the generation and maintenance mechanisms of these flow patterns is a requirement for a number of environmental and ecological processes including the transport and fate of sediment, pollutants, buoyant river discharge and anthropogenic material introduced to the coastal zone. This dissertation examines inner shelf and surf zone circulation patterns through: (a) the development and validation of appropriate techniques to incorporate the effect of …

Environmental Pre-Conditioning Of Bacterial Populations And Communities To Extreme Acidity, Gabriel Joseph Swenson

Theses and Dissertations

The ability of opportunistic enteric pathogens to cause disease is effected by their ability to survive the variable and lethal acidity of the mammalian host gastrointestinal milieu. In many of these pathogens, survival is conferred by an acid stress response (ASR). ASR is elicited when bacterial cells are pre-conditioned in sub-lethal acidity (pH 4-6) which induces rapid biochemical and physiological modifications and facilitates survival when extreme acidity (pH 1-3) is encountered. In Vibrio cholerae, the causative agent of the diarrheal disease Cholera, ASR was initially investigated in an effort to determine how this ubiquitous aquatic bacterium is capable of causing …

Insects As Indicators Of Environmental Stress, De Anna Estella Beasley

Theses and Dissertations

In the face of climate change-related events and anthropogenic disturbances, understanding the impacts of these events on species richness, abundance and distribution is important for us to mitigate biodiversity loss and better predict consequences for the environment and for human life. Insects are excellent models for understanding the consequences of environmental stress due to the sensitivity of their development and behavior. The overall focus of my dissertation research is the investigation of fluctuating asymmetry and oviposition behavior as indicators of environmental stress. My research includes a meta-analysis of published literature, and empirical research that was part of a collaborative initiative …

Outsmarting The Brain: Augmenting Motor Training With Non-Invasive Brain Stimulation In Order To Facilitate Plasticity-Dependent, Functional Improvement Within The Motor Cortex, Raymond Joseph Butts

Theses and Dissertations

Brain stimulation techniques capable of optimizing cortical plasticity may provide the key to improved therapeutic techniques and functional outcomes. The primary aim of this dissertation was to examine the potential of motor training (MT) augmented with intermittent theta burst stimulation (iTBS) and anodal transcranial direct current stimulation (a-tDCS). The secondary aim was to investigate whether the training would also be advantageous to older-adults. We hypothesized that right-handed, college-age students exposed to the treatment (n=17) would perform better short-term (directly following MT) and long-term (24 hours and 7 days following MT) on motor-skill retention tests than students receiving sham stimulation (n=14). …

A Qualitative Study On How Prenatal Genetic Counselors Prioritize Cultural Issues When Counseling Patients, Darcy Katherine Berry

Theses and Dissertations

The genetic counseling community has long recognized that the successful outcome of a session with a patient whose cultural background differs from the genetic counselor's is best achieved through recognition of the cultural differences and implementation of specific skills to overcome cultural barriers. Although research has examined the need and effects of cultural competency in genetic counseling, no prior study has evaluated how genetic counselors perceive the importance of addressing cultural issues in a genetic counseling session. Prenatal genetic counselors were recruited by emailing all NSGC members. For this study, sixteen prenatal genetic counselors were interviewed and asked to prioritize …

Unique Perspectives And Struggles Of Parents Rearing Children With Phenylketonuria With Unaffected Siblings, Cassandra Nicole Hollinger

Theses and Dissertations

Phenylketonuria (PKU) is an autosomal metabolic condition that is screened for via newborn screening. Individuals who are identified as having PKU require a protein-restricted diet to protect against neurological damage. Many parents who learn their child has PKU may already have children who are not affected, or may have children later who are not affected. This creates a unique situation in which parents are rearing children that require a strict protein-restricted diet with children who do not. Parents who are currently rearing children with and without PKU were surveyed to learn what unique perspectives and struggles they face, especially regarding …

Working With Patients At Risk For Hereditary Degenerative Brain Disorders, Stephen John White

Theses and Dissertations

Hereditary degenerative brain disorders (HDBDs) are a unique class of genetic conditions that result in progressive loss of function within the nervous system, many of which present during adulthood. Given this, the diagnosis of a HDBD can be daunting for both the patient and the genetic counselor assisting in medical care. The purpose of this study was to find themes among genetic counselors who see this patient population and help provide a framework to counselors entering this field by recognizing verified methods of HDBD counseling. Sixteen genetic counselors who routinely see patients at-risk for HDBDs were interviewed concerning how they …

Quantifying The Effects Of Antibiotics Upon Benthic Diatoms And Marine Benthic Algal Communities, Isaac M. Hagenbuch

Theses and Dissertations

Antibiotics are a cornerstone of modern medicine. Their ability to impair prokaryotic metabolism has saved the lives of many millions of humans in the decades since wide antibiotic usage began. Antibiotic compounds are also widely used in the husbandry of animals that are used for food but, unfortunately, much of the antibiotic mass that goes into humans and animals is excreted unchanged. Those excretions go, directly or indirectly, into aquatic ecosystems. The discovery of this fact has led to a growing global concern about the fate of antibiotics in the environment. This concern is driven by no fewer than three …

The Reproductive Ecology Of Plethodontid Salamanders In The South Carolina Inner Coastal Plain, Heather Rena Oswald

Theses and Dissertations

Nest- site selection is a critical process in the life history of amphibians, directly influencing offspring survival and parental fitness. When and where an amphibian chooses to nest is strongly influenced by a range of environmental cues. We monitored plethodontid salamander breeding behavior in seepage wetlands in the South Carolina inner Coastal Plain. Seepage wetlands are ideal for monitoring semiaquatic salamander breeding, because they provide habitat for both larval and adult salamanders while having relatively predictable, constant water temperatures and presence year round. We observed the breeding phenology of three sympatric salamanders (Eurycea cirrigera, Desmognathus auriculatus, and Pseudotriton ruber) in …

Optimal Cooperation In Joint Action Tasks, Scott Michael Blanchette

Theses and Dissertations

Joint action can be succinctly defined as an action in which two or more individuals work together for a common outcome. There have been numerous studies on the mechanisms behind joint action, but none have focused on the existence of optimal cooperators. The present study investigated if optimal cooperators, individuals who are better partners in joint action tasks, exist in the population. Individuals completed a virtual bar balancing task in dyads in which four hands were used to control the bar. Based on success rate, significant differences in performance variables were found. This study provides empirical evidence that some individuals …

Measuring Organizational Member Involvement In Physical Activity Coalitions Across The United States, Daniel Benjamin Bornstein

Theses and Dissertations

Physical inactivity has been labeled a global pandemic with enormous economic, social, environmental, and public health consequences. The vast majority of American adults and youth are insufficiently physically active. Increasing population-levels of physical activity (PA) requires broad social and environmental change. PA coalitions provide the structure through which such broad change can be achieved. An extensive literature on health-based coalitions suggests that coalition success is impacted by coalition members. Coalitions are generally comprised of member organizations. Little is known about the characteristics of PA coalitions and less is known about organizational member involvement in PA coalitions. The purpose of this …

The Effect Of Thymidylate Synthase Inhibitors On Bone Marrow Derived Cells In The Intestinal Tumor Microenvironment, Grishma Acharya

Theses and Dissertations

Tumors have come to be known as independently functioning complex organs consisting of cancer cells coexisting with a heterogeneous mixture of host-derived non-neoplastic cells that form the tumor stroma. Tumor survival, progression, and metastasis depend on multiple close interactions between the cancer cells and the tumor stromal cells. These tumor stromal cells are mainly bone marrow derived cells (BMDCs) that are recruited to the primary tumor and sites of metastasis by a variety of signals secreted by the cancer cells. Because of the close interaction between the tumor and the tumor stroma, we propose that tumor stromal cells play an …

Estimation Of Aerosolized Antibiotic Resistant Bacteria Concentrations From Wastewater Treatment Facilities Using Spatially-Based Dispersion Modeling, Renee Nichole Dickman

Theses and Dissertations

Pathogenic bacteria can develop resistance to antibiotics to which they were previously sensitive, resulting in increased morbidity and mortality worldwide [1]. The increase in drug resistance in bacteria is an emerging public health concern. The activated sludge tanks of wastewater treatment plants are known sources of antibiotic resistant bacteria. These resistant bacteria can become aerosolized and disperse downwind. Using previously sampled aerosol concentrations and meteorological data, a Gaussian dispersion model was developed to estimate the concentration downwind from a selected wastewater treatment facility. The emission rate was calculated to be 7,941 CFU/m²/sec ± 2,149 CFU/m²/sec. This value was used in …

Examining The Differences In Rapport Between Male And Female Cancer Genetic Counselors And Female Clients, John Abernethy

Theses and Dissertations

Genetic counseling is a field in which client-counselor rapport plays a critical role in client satisfaction with the genetic counseling process. One factor that may impact this rapport is gender of the genetic counselor. Previous studies in the field of psychological counseling suggest that gender is not a significant moderator of this rapport. To the best of our knowledge, no study has been published in the field of genetic counseling examining the impact that the gender of the genetic counselor has on client-counselor rapport. To study this effect, an amended version of Horvath & Greenberg's (1989) Working Alliance Inventory tool …

The Association Of Physical Fitness With Psychological Health Outcomes In Soldiers During Army Basic Combat Training, Shannon K. Crowley

Theses and Dissertations

Despite the current need for investigation of factors involved with soldiers' resilience to stressors in the Basic Combat Training (BCT) environment, and evidence in civilian populations that physical training is associated with psychological benefits, little is known about the relationship between physical fitness and psychological adjustment during BCT. Study one of this dissertation involved an extensive literature review of factors related to the relationship between physical fitness and psychological adjustment of soldiers during BCT. Using qualitative focus group methods, study two assessed soldiers' perceptions about their sleep, and consequences of sleep disruption during BCT at Fort Jackson in Columbia, SC. …

Modeling Approaches, Physiological Responses, And Climate Change: How Good Is "Good Enough?", Nicole Kish

Theses and Dissertations

Predicting spatial and temporal patterns in the responses of organisms and ecosystems to climate change has emerged as a major focus of macrophysiological research, with much work centered on the impacts of temperature. A potential difficulty lies in the observation that measures of 'habitat' such as air, land and sea surface temperature often differ greatly from the body temperature actually experienced by organisms, as the latter drives reproduction and survival. As a result, it is unclear how often these simple measurements of habitat are 'good enough' for predicting physiological stress in the field, and when more complicated methods are needed. …