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Assembly Of Reca-Like Recombinases: Distinct Roles For Mediator Proteins In Mitosis And Meiosis, Stephen L. Gasior
Assembly Of Reca-Like Recombinases: Distinct Roles For Mediator Proteins In Mitosis And Meiosis, Stephen L. Gasior
Biological Sciences Faculty Publications
Members of the RecA family of recombinases from bacteriophage T4, Escherichia coli, yeast, and higher eukaryotes function in recombination as higher-order oligomers assembled on tracts of single-strand DNA (ssDNA). Biochemical studies have shown that assembly of recombinase involves accessory factors. These studies have identified a class of proteins, called recombination mediator proteins, that act by promoting assembly of recombinase on ssDNA tracts that are bound by ssDNA-binding protein (ssb). In the absence of mediators, ssb inhibits recombination reactions by competing with recombinase for DNA-binding sites. Here we briefly review mediated recombinase assembly and present results of new in vivo experiments. …
Acclimation To Hypoxia Increases Survival Time Of Zebrafish, Danio Rerio, During Lethal Hypoxia, Bernard B. Rees, F Sudradjat, J W. Love
Acclimation To Hypoxia Increases Survival Time Of Zebrafish, Danio Rerio, During Lethal Hypoxia, Bernard B. Rees, F Sudradjat, J W. Love
Biological Sciences Faculty Publications
Survivorship of zebrafish, Danio rerio, was measured during lethal hypoxic stress after pretreatment in water at either ambient oxygen or at a lowered, but nonlethal, level of oxygen. Acclimation to nonlethal hypoxia (pO2 @ 15 Torr; ca. 10% air-saturation) for 48 hr significantly extended survival time during more severe hypoxia (pO2 @ 8 Torr; ca. 5% air-saturation) compared to survival of individuals with no prior hypoxic exposure. The magnitude of the acclimation effect depended upon the sex of the fish: hypoxia pretreatment increased the survival times of males by a factor of approximately 9 and that of females by a …