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Full-Text Articles in Life Sciences
The Transcription Factors Adr1 Or Cat8 Are Required For Rtg Pathway Activation And Evasion From Yeast Acetic Acid-Induced Programmed Cell Death In Raffinose, Zhengchang Liu, Luna Laera, Nicoletta Guaragnella, Maša Ždralević, Domenico Marzulli, Sergio Giannattasio
The Transcription Factors Adr1 Or Cat8 Are Required For Rtg Pathway Activation And Evasion From Yeast Acetic Acid-Induced Programmed Cell Death In Raffinose, Zhengchang Liu, Luna Laera, Nicoletta Guaragnella, Maša Ždralević, Domenico Marzulli, Sergio Giannattasio
Biological Sciences Faculty Publications
Yeast Saccharomyces cerevisiae grown on glucose undergoes programmed cell death (PCD) induced by acetic acid (AA-PCD), but evades PCD when grown in raffinose. This is due to concomitant relief of carbon catabolite repression (CCR) and activation of mitochondrial retrograde signaling, a mitochondria-to-nucleus communication pathway causing up-regulation of various nuclear target genes, such as CIT2, encoding peroxisomal citrate synthase, dependent on the positive regulator RTG2 in response to mitochondrial dysfunction. CCR down-regulates genes mainly involved in mitochondrial respiratory metabolism. In this work, we investigated the relationships between the RTG and CCR pathways in the modulation of AA-PCD sensitivity under glucose repression …
Mitochondrial Dna Instability In Cells Lacking Aconitase Correlates With Iron Citrate Toxicity, Zhengchang Liu, Fei Xiao, Zhejun Dong, Tammy Pracheil, Muhammad Farooq
Mitochondrial Dna Instability In Cells Lacking Aconitase Correlates With Iron Citrate Toxicity, Zhengchang Liu, Fei Xiao, Zhejun Dong, Tammy Pracheil, Muhammad Farooq
Biological Sciences Faculty Publications
Aconitase, the second enzyme of the tricarboxylic acid cycle encoded by ACO1 in the budding yeast Saccharomyces cerevisiae, catalyzes the conversion of citrate to isocitrate. aco1Δ results in mitochondrial DNA (mtDNA) instability. It has been proposed that Aco1 binds to mtDNA and mediates its maintenance. Here we propose an alternative mechanism to account for mtDNA loss in aco1Δ mutant cells. We found that aco1Δ activated the RTG pathway, resulting in increased expression of genes encoding citrate synthase. By deleting RTG1, RTG3, or genes encoding citrate synthase, mtDNA instability was prevented in aco1Δ mutant cells. Increased activity of citrate synthase leads …