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Full-Text Articles in Life Sciences
Population Proteomics: Quantitative Variation Within And Among Populations In Cardiac Protein Expression, Bernard Rees
Population Proteomics: Quantitative Variation Within And Among Populations In Cardiac Protein Expression, Bernard Rees
Biological Sciences Faculty Publications
Population analysis of gene expression is typically achieved by quantifying levels of mRNA; however, gene expression is also a function of protein translation and turnover. Therefore, a complete understanding of population variation in gene expression requires quantitative knowledge of protein expression within and among natural populations. We used two dimensional fluorescence difference gel electrophoresis (2D-DIGE) to quantitatively compare expression of heart ventricle proteins among 18 individuals in three populations of the teleost fish Fundulus. Among populations, expressions between orthologous proteins and mRNAs were generally positively correlated. Additionally, similar to the pattern of cardiac mRNA expression for the same populations, we …
Oxygen Limitation And Tissue Metabolic Potential Of The African Fish Barbus Neumayeri: Roles Of Native Habitat And Acclimatization, Bernard Rees
Biological Sciences Faculty Publications
Oxygen availability in aquatic habitats is a major environmental factor influencing the ecology, behaviour, and physiology of fishes. This study evaluates the contribution of source population and hypoxic acclimatization of the African fish, Barbus neumayeri, in determining growth and tissue metabolic enzyme activities. Individuals were collected from two sites differing dramatically in concentration of dissolved oxygen (DO), Rwembaita Swamp (annual average DO 1.35 mgO2 L-1) and Inlet Stream West (annual average DO 5.58 mgO2 L-1) in Kibale National Park, Uganda, and reciprocally transplanted using a cage experiment in the field, allowing us to …
Biosynthesis Of Cyanobacterial Phycobiliproteins In Escherichia Coli: Chromophorylation Efficiency And Specificity Of All Bilin Lyases From Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter
Biosynthesis Of Cyanobacterial Phycobiliproteins In Escherichia Coli: Chromophorylation Efficiency And Specificity Of All Bilin Lyases From Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter
Biological Sciences Faculty Publications
No abstract provided.
Effects Of Modified Phycobilin Biosynthesis In The Cyanobacterium Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter
Effects Of Modified Phycobilin Biosynthesis In The Cyanobacterium Synechococcus Sp. Strain Pcc 7002, Wendy M. Schluchter
Biological Sciences Faculty Publications
No abstract provided.
Attachment Of Non-Cognate Chromophores To Cpca Of Synechocystis Sp. Strain Pcc 6803 Expressed In E. Coli, Wendy M. Schluchter
Attachment Of Non-Cognate Chromophores To Cpca Of Synechocystis Sp. Strain Pcc 6803 Expressed In E. Coli, Wendy M. Schluchter
Biological Sciences Faculty Publications
No abstract provided.
Geographic Variation In The Effects Of Heat Exposure On Maximum Sprint Speed And Hsp70 Abundance In Populations Of The Western Fence Lizard, Scelopolus Occidentalis, D M. Mcmillan, D J. Irschick, Bernard B. Rees
Geographic Variation In The Effects Of Heat Exposure On Maximum Sprint Speed And Hsp70 Abundance In Populations Of The Western Fence Lizard, Scelopolus Occidentalis, D M. Mcmillan, D J. Irschick, Bernard B. Rees
Biological Sciences Faculty Publications
We examined whether western fence lizards Sceloporus occidentalis
occurring in thermally divergent environments display differential
responses to high temperature in locomotor performance
and heat-shock protein (Hsp) expression. We measured
maximum sprint speed in S. occidentalis from four populations
at paired latitudes and elevations before and after exposure to
an experimental heat treatment and then quantified hind-limb
muscle Hsp70 expression. Lizards collected from northern or
high-elevation collection sites suffered a greater reduction in
sprint speed after heat exposure than lizards collected from
southern or low-elevation sites. In addition, lizards from northern
collection sites also exhibited an increase in Hsp70 expression
after …
Oxygen Limitation And Tissue Metabolic Potential Of The African Fish Barbus Neumayeri: Roles Of Native Habitat And Acclimatization, Martinez M. L, Raynard E. L, Bernard B. Rees, Chapman L. J
Oxygen Limitation And Tissue Metabolic Potential Of The African Fish Barbus Neumayeri: Roles Of Native Habitat And Acclimatization, Martinez M. L, Raynard E. L, Bernard B. Rees, Chapman L. J
Biological Sciences Faculty Publications
Background: Oxygen availability in aquatic habitats is a major environmental factor influencing the ecology, behaviour, and physiology of fishes. This study evaluates the contribution of source population and hypoxic acclimatization of the African fish, Barbus neumayeri, in determining growth and tissue metabolic enzyme activities. Individuals were collected from two sites differing dramatically in concentration of dissolved oxygen (DO), Rwembaita Swamp (annual average DO 1.35 mgO2 L-1) and Inlet Stream West (annual average DO 5.58 mgO2 L-1) in Kibale National Park, Uganda, and reciprocally transplanted using a cage experiment in the field, allowing us to maintain individuals under natural conditions of …