Life Sciences Commons^™

Unsung Heroes In Conservation: Evaluating The Limitations Faced By New England Wildlife Rehabilitation Centers And Proposing Solutions For Their Support And Recognition, Shaylee M. Sarmiento

Honors Theses and Capstones

Wildlife rehabilitation is an often-unacknowledged practice within the overarching field of wildlife protection and conservation. This practice involves the treatment and hopeful release of wild animals affected by various ailments. With the continued expansion of and interest in the field, it could be assumed that wildlife rehabilitation is supported and funded by government bodies, but this is not the case. Because of the lack of funding and resources, many wildlife rehabilitation centers cannot achieve their full rehabilitative potential and expand their reach in the conservation world. Additionally, an absence of support leads critics to question the ethical standards of wildlife …

Taxonomic Classification Of Viral And Bacterial Dna Following 2021 Avian Mass Mortality Event, Tessa Baillargeon

Honors Theses and Capstones

From May through July 2021, an unusual mortality event occurred along the eastern coast and Midwest of the United States. Thousands of birds, mostly from the order Passeriformes, were part of the die-off including blue jays (Cyanocitta cristata), common grackles (Quiscalus quiscula), European starlings (Sturnus vulgaris), American robins (Turdus migratorius). Clinical signs included crusted eyes, swollen conjunctiva, otitis, seizures, and ataxia.

The New Hampshire Veterinary Diagnostic Laboratory (NHVDL) received over 100 affected birds from various collaborators throughout the United States including Washington DC, NJ, CT, MD, and OH. Given the timing and geologic …

Investigating The Relationship Between Sea Ice Extent And Erignathus Barbatus Breeding Phenology In The Alaskan Arctic, Rachel E. Lewis

Honors Theses and Capstones

No abstract provided.

Individual Behavioral And Neurobiological Markers Associated With A Vulnerable To Ethanol Use Phenotype, Kelsey M. Alimandi

Honors Theses and Capstones

Alcohol use disorder (AUD) is a chronic relapsing brain condition that is characterized by excessive alcohol consumption, continued use when faced with negative consequences, and a negative emotional state associated with withdrawals (anxiety, irritability, depression). The main challenge to treating AUD is preventing relapse. The purpose of this study was to use a prolonged-exposure model to allow rats to self-administer ethanol to determine the brain regions active during relapse events. The rats performed multiple behavioral tests such as economic demand, negative consequences, and an elevated plus maze. These tests determined how hard rats were willing to work for an ethanol …

The Prevalence And Impact Of Dairy/Beef Crosses On Cattle Producers In New Hampshire, Eleanor J. Braun

Honors Theses and Capstones

Recent years had seen a significant rise in the use of beef semen on dairy cows in the United States. This trend was not widely understood, especially as it related to dairy farmers in New Hampshire. A survey was sent out by mail to the ninety-seven dairy farms in New Hampshire. There were thirty-seven responses to the survey. Thirty-two out of thirty-seven respondents bred beef semen to their dairy cows. Twenty-one of these respondents raised dairy x beef on their farms and twenty-six planned to continue breeding dairy x beef. The most common breeds used in dairy x beef crosses …

Assessing The Bioavailability Of Infused Lysine And Rumen-Protected Lysine Supplements Using The Area Under The Curve Technique And The Plasma Free Amino Acid Dose-Response Method, Megan Vetter

Honors Theses and Capstones

The milk production of lactating dairy cows is dependent on factors such as housing conditions, lineage, climate, and health, but the quality of their diets is generally the most influential. Maintaining a proper balance of nutrients is necessary to achieve the greatest milk production at the lowest cost. Maximum feed efficiency is not only critical for increasing the economic profits of an individual farm, but also for increasing food supply without increasing environmental demand. Supplementing cows’ diets with lysine (Lys), an essential amino acid (AA), can aid in maximizing protein synthesis. Providing this nutrient in a rumen-protected (RP) coating can …

Revolutionary Advances In The Treatment Of Genetic Disease, Emma Kaitlyn Carrigan

Honors Theses and Capstones

No abstract provided.

Meta-Analytic Connectivity Modelling Of Healthy Swallowing, Chris R. Tilton

Honors Theses and Capstones

A quantitative, voxel-wise meta-analysis was performed to investigate the brain regions involved in healthy human swallowing. Studies included in the meta-analysis (1) examined water swallowing, saliva swallowing, or both, (2) included healthy, normal subjects, and (3) reported stereotaxic brain activation coordinates in standard space. Following these criteria, a systematic literature review identified 8 studies that met the criteria. An activation likelihood estimation (ALE) meta-analysis and meta-analytic connectivity modelling (MACM) analysis were performed with BrainMap software. Ten clusters with high activation likelihood were found in the bilateral precentral gyri, right insula, left declive, right medial frontal gyrus, right dorsal nucleus of …

Cutaneous Microbiome Of Red Fox (Vulpes Vulpes) Infected With Sarcoptic Mange (Sarcoptes Scabiei), Jacqueline C. Robidoux

Honors Theses and Capstones

Sarcoptic mange is a parasitic skin disease that affects countless mammals worldwide, including red foxes (Vulpes vulpes). The symptoms, such as hair loss, crusting, and the number of mites, vary between individual foxes. Sarcoptic mange damages the skin barrier, which in turn disrupts the biodiversity of bacteria in the microbiome. It is unknown what the biodiversity of bacteria is at each stage of the disease. This experiment will compare the microbiomes of different samples with and without mange; in hopes to reveal a connection between the different severities of sarcoptic mange and the biodiversity of bacteria and fungi …

Nutritive Comparison Of Ruminant Feed, Integrating Crab And Lobster Meal, Kelsi L. Devolve

Honors Theses and Capstones

In attempts to increase livestock production and decrease waste products from the seafood industry, crab meal and lobster meal were tested as a potential cattle feed ingredient. Jonah crab waste, Atlantic Red crab waste, soybean meal, and blood meal were collected from various vendors and utilized in an in vitro study, an in situ study, and a nitrogen analyzer to determine crude protein in each feed. After allowing the samples to sit in a DAISY incubator for 48 h, mimicking a rumen environment, degradability for the soybean meal, blood meal, lobster meal, and crab meal were 100.13%, 76.36%, 43.77%, and …

Eeg-Based Spanish Language Proficiency Classification: An Eeg Power Spectrum And Cross-Spectrum Analysis, Blaise Xavier O'Mara, Skyler Baumer

Honors Theses and Capstones

Second language proficiency may be predicted with electrophysiological techniques. In a machine learning application, this electrophysiological data may be used for language instructors and language students to assess their language learning. This study identifies how electroencephalogram (EEG) power spectrum and cross spectrum data of the brain cortex relates to Spanish second language (L2) proficiency of 20 Spanish language students of varying proficiency levels at the University of New Hampshire. The two metrics for assessing cortical power and processing were event-related desynchronization (ERD)—a measure of relative change in power—of the alpha (8-12 Hz) brain frequency band, and alpha and beta (13-30Hz) …

The Impact Of Nutrient Depletion On The Effect Of Hhq On Emiliania Huxleyi, Marley Gonsalves

Honors Theses and Capstones

Emiliania huxleyi is a ecologically important, bloom-forming coccolithophore. HHQ is a bacteria compound that causes cell stasis in E. huxleyi that can lead to bloom termination. This study analyzed the role that nutrient depletion plays in this relationship. Results show that nitrogen depletion protects cells from the effects of HHQ, affecting bloom dynamics.

Targeting Stat3 In Triple Negative Breast Cancer Using Gsk3Ꞵ And Integrin Inhibitors, Emily A. Pratt

Honors Theses and Capstones

Triple negative breast cancer (TNBC) is the most aggressive subtype of breast cancer and is known for its chemoresistance and high rate of recurrence. TNBC lacks the hormone receptors that are found in other types of breast cancer, and therefore cannot be treated with hormonal therapies. Although the initial response to chemotherapy is favorable, many TNBC patients experience a recurrence of this cancer at a secondary location and this recurrence is often more lethal than the original tumor. The Signal Transducer and Activator of Transcription (STAT) pathway is a major contributor to cancer growth and metastasis, and STAT3 is constitutively …

Investigation Of Mechanical Regulation On Stat3 Activity And Mmp Production, Jaxson R. Libby

Honors Theses and Capstones

Transcription factor, STAT3, is inappropriately expressed in cancer cells, and has contrasting activation in 2D versus 3D microenvironments. 2D plates are often used for drug screening and do not always recapitulate in vivo responses. To combat inaccurate 2D drug studies, a 3D hydrogel was created to support the growth of cancer cells into a tumor-like environment. The hydrogel consists of a biocompatible dextran homopolysaccharide, cell adhesion RGD sequences, and crosslinker MMP labile peptides. A pH dependent reaction couples the RGD sequences to dextran then the polymers are crosslinked into a gel. Crosslinking is accomplished using terminal cysteine peptide sequences, allowing …

Investigation Of The Function Of Protein Acyl Transferases Using Crispr-Cas9 To Create Null Mutants, Claire E. Christopher

Honors Theses and Capstones

24 Protein Acyl Transferases (PATs) have been identified in the model organism Arabidopsis thaliana. Despite knowing the enzymatic function of these genes (palmitoylation), the specific subcellular pathways, protein targets, and cellular activities of these proteins remain unknown. To investigate the pathways that PAT genes are involved in, deletion mutations were created in several genes and the phenotype of the organisms carrying homozygous mutations was observed. To introduce mutations, the CRISPR/Cas9 system was inserted into the plant’s genome using Agrobacterium tumefaciens to cause deletions in PAT genes of interest. To date, no CRISPR-induced pat mutants have a noticeable phenotype. These …

The Relationship Between Alpha-Defensin 1 And Gut Microbiome Composition In A Population Of Bhutanese Refugee Adults With A High Prevalence Of Type 2 Diabetes, Daisha Alana Drake

Honors Theses and Capstones

The New Hampshire Bhutanese refugee population is disproportionately affected by obesity and metabolic disorders such as type 2 diabetes. The gut microbiome is important for overall human health, with high microbial richness and diversity being associated with reduced chronic inflammation and type 2 diabetes risk. Microbial richness and diversity have been shown to decrease in United States (U.S.) immigrant populations, but have not been measured in refugee populations. One of the anti-inflammatory functions of the gut microbiome is related to the production of short-chain fatty acids (SCFAs), which regulate pro-inflammatory cytokine production. Alpha-defensin 1 is an antimicrobial peptide that is …

Neuronal Primary Cilia In Postnatal Brains & Alzheimer’S Disease Mice Eeg Patterns Under Fear Conditioning, Sierra Rose Mae Walsh

Honors Theses and Capstones

Alzheimer’s Disease (AD) is a neurodegenerative condition caused by the abnormal accumulation of amyloid β plaque. While small amounts of amyloid β plaque is to be expected with increased age, AD presents amyloid precursor protein (APP), a gene promoting inappropriate plaque formation, causing early neuronal death and tissue depreciation. APP23 transgenic mouse models contain the human mutation in the neocortical and hippocampal positions, areas revealing of plaque accumulation and memory loss. Within the hippocampal cortices, the primary cilia, which regulate higher cognition and neurodevelopment can be studied along with burst suppressions, or moments of high focal activity, to target potential …

Investigating The Role Of Bromodomain Proteins On Histone Post-Translational Modifications In Toxoplasma Gondii, Joshua Steven Berthiaume

Honors Theses and Capstones

Toxoplasma gondii is a prevalent pathogenic parasite that infects approximately 25 percent of the US population. For the parasite to successfully establish and maintain infection in its host, properly controlled regulation of gene expression is critical. One way Toxoplasma regulates gene expression is through modification of histone proteins that bind to DNA and can control gene accessibility. Acetylation is a modification that is added to histones that changes chromatin structure to enhance gene activation. Histone acetylation can also regulate gene expression by recruitment of important regulators such as bromodomain proteins (BDP). A previous study showed that loss of the bromodomain …

Effects Of Aging On The Reproductive Success Of Female Saltmarsh Sparrows (Ammospiza Caudacutus) In Maine And New Hampshire, Isabella Rose Collamati

Honors Theses and Capstones

The saltmarsh sparrow (Ammospiza caudacutus) is a ground-nesting specialist in coastal salt marshes of the Northeast. Rising sea-levels increase the loss of offspring due to tidal flooding, reducing nest success and resulting in a sharp population decline. In other avian species, age has been shown to affect nest success through altering fertility, behavior, and the number of young produced, favoring older individuals. I investigated age effects on nest success of female saltmarsh sparrows using nest monitoring data collected at four sites of a long-term demographic monitoring project: Chapman's Landing (Stratham, NH), Eldridge Marsh (Wells, ME), Popham Beach (Phippsburg, …

Identification Of Novel Biosynthetic Gene Clusters Encoding For Polyketide/Nrps-Producing Chemotherapeutic Compounds From Marine-Derived Streptomyces Hygroscopicus From A Marine Sanctuary, Hannah Ruth Flaherty

Honors Theses and Capstones

Nearly one out of six deaths in 2020, around ten million people, were caused by cancer, making it a leading cause of death worldwide (WHO, 2022). This major public health issue, in addition to the rise of multidrug-resistant (MDR) pathogens, provides a high demand for the discovery of new pharmaceutical drugs to be used clinically to treat these conditions. The Streptomyces genus accounts to produce 39% of all microbial metabolites currently approved for human health, indicating its potential as an important species to study for antimicrobial and anticancer agents. The long linear genome of Streptomyces contains specialized sequences known as …

The Effects Of Bacillus Velezensis Ncimb 30322 On Growth Of Chenopodium Ficifolium In Nutrient-Depleted Conditions, Ciana B. Lazu

Honors Theses and Capstones

Abiotic stressors, such as nutrient and drought stress, are a leading cause of crop yield reduction. Some bacteria and fungi are able to promote plant growth and increase stress tolerance by increasing nutrient availability through nitrogen fixation or phosphate solubilization, outcompeting pathogens, productions of volatile organic compounds (VOCs), reducing plant stress, and inducing induced systemic resistance (ISR), and thus serve as a valuable component of agricultural ecosystems to reduce dependency on chemical fertilizers. Chenopodium quinoa is a crop native to South America that has been gaining popularity in the United States because of its nutritive properties and resistance to drought …

Dog Weight, Age, And Sex Relationship With Shelter Length-Of-Availability At Pope Memorial Humane Society, Logan Lajoie

Honors Theses and Capstones

Many factors can influence length-of-availability for dogs in shelters, ranging from factors of the dogs themselves to outside factors. The external factors could be the geographical location of the shelter and its clientele, the socioeconomic status of the area, how the individual shelter uses its resources, the region's policies, and more (Scarlett, 2021, p. 18). This study evaluated length-of-availability in dogs at the Pope Memorial Humane Society, a nonprofit animal shelter that serves Strafford County, New Hampshire. This allowed for dog-specific factors to be investigated more closely. The specific factors investigated were dog weight, age, and sex compared to their …

Characterization Of L-Malic Acid-Capped Cds Quantum Dots And Examination Of The Nanoncrystal's Biosensor Ability, Andrei A. Potapenko

Honors Theses and Capstones

Ligand-capped quantum dots are microscopic semiconductors commonly used in biomedical research and therapy for cell imaging and drug delivery. Despite the abundance of in vivo applications, the nature of interactions between the nanocrystals and the amino acids comprising human proteins remains insufficiently investigated. In this work, we report on the synthesis, ligand exchange, and characterization of the L-malic acid-capped CdS quantum dots via various spectroscopic techniques. We also assess the ligand-capped nanocrystals as potential probes for some amino acids. We determine that in the presence of L-cysteine, the L-malic acid-capped CdS quantum dots fluorescence signal quenches drastically, thus indicating a …

The Effect Of Post-Meal Walking On 24-Hour Central Blood Pressure In Young Women With And Without Excess Adiposity, Maison P. D'Amelio, Bilal A. Chaudhry, Ciana B. Lazu

Honors Theses and Capstones

Post-meal walking (PMW) performed after breakfast, lunch, and dinner has been demonstrated to reduce blood glucose. However, no studies have examined the potential additive benefits of post-meal walking exercise on daytime central blood pressure (BP) in young women. METHODS: Thirteen physically inactive, non-hypertensive women (Age: 20±1 years; percent body fat: 28.2±13%) completed the study during the early follicular or placebo phase of their contraceptive cycle. Participants completed a control day (CON; no exercise/excess physical activity) and PMW day (3 bouts x 15 minutes of brisk walking) over five days in random order. Daytime ambulatory BP and accelerometry data (to estimate …

Impact Of Drought Stress On Oak Stomatal Size And Density At Thompson Farm, Nh, Emily Chen

Honors Theses and Capstones

Global climate change is likely to affect northeastern U.S. forests by increasing the frequency and severity of drought events. Historically, droughts rarely occurred in this region, so native tree species are not well-adapted to extreme moisture stress. In the future, the changing climate will likely cause unprecedented levels of low water availability which may have implications on future forest composition. It is hypothesized that trees can respond to these environmental changes by altering their functional traits, also referred to as phenotypic plasticity; for example, by producing leaves with fewer and smaller stomata.

To determine the capacity to which oak trees …

Investigating The Main Protease (Mpro) Of Sars-Cov-2 As A Potential Drug Target, Valerie Giovina Pascetta

Honors Theses and Capstones

The rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of the coronavirus disease 19 (COVID-19) pandemic has claimed the lives of roughly 6.2 million people worldwide as of May 2022. The virus’s main protease (M^pro ) has been identified as an attractive drug target due to the critical role it plays in the viral life cycle. The roughly 34 kDa M^pro cleaves functional viral polypeptides out of two long polyproteins at conserved cut sites, allowing them to fulfill their role in processes like transcription and replication. Here, we have studied the enzymatic activity …

Finding The Best Predictors For Foot Traffic In Us Seafood Restaurants, Isabel Paige Beaulieu

Honors Theses and Capstones

COVID-19 caused state and nation-wide lockdowns, which altered human foot traffic, especially in restaurants. The seafood sector in particular suffered greatly as there was an increase in illegal fishing, it is made up of perishable goods, it is seasonal in some places, and imports and exports were slowed. Foot traffic data is useful for business owners to have to know how much to order, how many employees to schedule, etc. One issue is that the data is very expensive, hard to get, and not available until months after it is recorded. Our goal is to not only find covariates that …

Targeting The Bcl6 Transcription Factor In Ovarian Cancer, Harrison H. Parent

Honors Theses and Capstones

Ovarian cancer is one of the most common reproductive malignancies for women in the United States, as well as one of the deadliest. Many deaths attributed to ovarian cancer, as well as cancer generally, are due to metastasis of the original tumor to different tissues throughout the body. The BCL6 transcription factor has been identified to play a key role in the metastasis of these tumors, as well as the invasion of these cancer cells through mesothelial cell layers, which contributes to ovarian cancer’s unique metastatic behavior. In an attempt to target these metastases and prevent mesothelial penetration, drugs were …

Screening Ocean Samples From Georgia For The Presence Of Msx And Dermo Using Pcr And Qpcr Methods, Olivia Michelle Williams

Honors Theses and Capstones

This Senior Thesis project aimed to be able to screen samples that came in from a PhD student in Georgia for the presence of two oyster diseases, MSX and Dermo. This study was done using PCR followed by visualizing on E-gels for initial presence of disease. These were then followed by qPCR and visualization on Criterion gels. These Criterion gels were able to be analyzed in order to calculate the pg/uL of MSX and Dermo in the original ocean samples. Since the samples were sent in over the course of February-August ‘21 the presence over time was also able to …

Occurrence Of Pulmonary Arteritis With Lungworm Infections In Gray Seals From New England, 2013-2020, Jillian R. Broadhurst, Inga Sidor Dvm, Ms, Dacvp

Honors Theses and Capstones

Gray seals (Halichoerus grypus) are a common pinniped species in New England coastal waters. Parasitic infection is an important cause of mortality for this species, and lungworm species are frequently implicated in cases of respiratory infection, especially in pups and weanling seals. The most common of these is Otostrongylus circumlitus, a large nematode found in the bronchi and bronchioles of affected seals. The full life cycle is unknown but its presence in lungs and sometimes pulmonary arteries suggests that migration via arterial circulation occurs. Signs and microscopic findings are widely variable; our cases included dyspnea, bloody mucoid …