Life Sciences Commons^™

Acute Salivary Steroid Hormone Responses During Coalitional And Dyadic Competitions In Hong Kong Juvenile Children, Timothy Mchale

UNLV Theses, Dissertations, Professional Papers, and Capstones

A large body of research links testosterone and cortisol responses to competition during adulthood. Little psychoneuroendocrine research has explored salivary steroid hormone responses to competition during middle childhood. This project investigated the relationship between acute salivary steroid hormone change, performance, competitor type, and outcome effects in three different field studies, while controlling for Body Mass Index (BMI) and pubertal development, in a population of ethnically Chinese, Hong Kong juvenile children, 8-11 years of age. The relative dynamics of salivary steroid change were assessed during a 1) coalitional physical competition (soccer) in boys, 2) a non-physical mixed-sex coalitional competition (math contest), …

Complete Sequencing And Comparative Analysis Of The Genomes Of The First Magnetotactic Gammaproteobacteria Isolated In Pure Culture: Strains Bw-2 And Ss-5, Corey Geurink

UNLV Theses, Dissertations, Professional Papers, and Capstones

The genomes of the first two discovered magnetotactic bacteria (MTB) belonging to the ammaproteobacteria, strains BW-2 and SS-51, were sequenced, sealed, annotated and compared to MTB of other phylogenetic groups. Cells of both strains are rod-shaped and biomineralize cuboctahedral and elongated octahedral crystals of magnetite, respectively, that are enveloped in a protein-embedded, lipid-bilayer membrane referred to as the magnetosome membrane or vesicle. The crystals and their associated membranes are known as magnetosomes. Magnetosome crystals consist of either magnetite (Fe3O4) or greigite (Fe3S4) and, because of their specific mineral compositions, crystal morphologies and sizes, the biomineralization processes involved in magnetosome formation …

Exercise, Learning And Emotional Health: Interdisciplinary Approaches To Translational Action, Tessa L. Koschel

UNLV Theses, Dissertations, Professional Papers, and Capstones

Exercise positively effects physical, psychological and neurological wellbeing. Though the population at large is becoming increasingly aware of the multi-faceted benefits of exercise, the majority of people fail to meet daily exercise recommendations. Largely, demands of everyday life such as work, school and family take priority over fitness. The issue therefore becomes a matter of time. In a world of media multi-tasking and immediate gratification, the challenge to health professionals becomes incorporation and manipulation of these consistencies to improve exercise uptake and adherence. Focus must shift from the idea of making additional time for exercise, to adding exercise to time …

An Analysis Of The Spatial, Temporal, And Functional Role Of Key Proteins In The Drosophila Melanogaster Salivary Gland During Exocrine Secretion, Kathryn Lantz

UNLV Theses, Dissertations, Professional Papers, and Capstones

For over 60 years, the larval salivary gland of Drosophila melanogaster has been the ultimate model for elucidating how steroid hormones trigger genome-wide changes in gene expression in a specific target tissue. Because of the unusual interphase chromosomes of the salivary gland, the regions of the genome that are being induced by the steroid/receptor complex actually form puffs that are easily visible in compound microscopy. Despite the importance of this system, little has been elucidated over the years regarding how the hormone exposure actually changes the physiological activity of this secretory system. The work presented in this dissertation makes a …

Software For Sequence Analysis Of Variants In Functional Screening Libraries And Personalized Genome Files, Jacklyn Michelle Newsome

UNLV Theses, Dissertations, Professional Papers, and Capstones

Detailed knowledge of protein function is critical for both the study of protein interactions and the development of drugs which target specific proteins. Currently, there are few techniques that directly examine protein function. The techniques that are available are time consuming and can only address one variant of a protein at a time. Our laboratory has designed 3 high throughput protein function screens. We hypothesize that these will address this shortfall.

The first screen is the Chimeric Minimotif Decoy (CMD) Assay. For this screen, we constructed red fluorescent proteins with one or more C-terminal minimotifs. Minimotifs are short, contiguous amino …

A Potential Solution To A Poopy Problem: Bile Salt Analogs As Prophylactics Against Clostridium Difficile Infection, Jacqueline Renee Phan

UNLV Theses, Dissertations, Professional Papers, and Capstones

Clostridium difficile infection (CDI) is a major cause of antibiotic-associated diarrhea. In 2011, over 500,000 patients were diagnosed with CDI in the United States and over 29,000 people died of CDI-related complications. With an average of $35,000 to treat a single case of inpatient CDI, cost burden to the healthcare system can reach up to $3.2 billion annually. As both hospital- and community-acquired CDI incidences rise due to the emergence of hypervirulent strains and CDI reoccurrences of up to 25%, standard treatments are rendered less effective and new methods of prevention are critical.

CDI is caused by bacteria called Clostridium …

Emerging Infectious Disease And The Imperiled Relict Leopard Frog, Anthony Wayne Waddle

UNLV Theses, Dissertations, Professional Papers, and Capstones

The disease chytridiomycosis, caused by the aquatic fungal pathogen Batrachochytrium dendrobatidis (Bd), has emerged as a major contributing factor for worldwide amphibian declines. Although relatively recently described, the impacts from the disease this pathogen causes have been definitively tied to amphibian declines, including some that occurred decades ago. In some cases, declines of individual species occurred with little documentation and are thus poorly understood. The relict leopard frog (Rana onca = Lithobates onca) has experienced such a decline and by the latter part of the 20th century only occurred in two general areas in southern Nevada. Recent research has found …

Dietary Intake And Energy Expenditure Of Pararescuemen During Routine Training, Andrea Woita

UNLV Theses, Dissertations, Professional Papers, and Capstones

Pararescuemen are one group of Air Force Special Operations Forces (SOF) who engage in vigorous physical training to achieve mission readiness. This type of training requires high energy expenditures and matching dietary intake to support basic physiological needs and to meet performance demands. However, few studies have investigated the energy expenditure and dietary intake of SOF and none have specifically examined these behaviors in Pararescuemen. The goal of this investigation was to determine the energy expenditure and dietary intake of Pararescuemen during routine training - a period when these operators receive supervision and guidance for performance improvements, recovery, and career …

Analysis Of Human Movement Patterns During Endurance Running, Joshua Paul Bailey

UNLV Theses, Dissertations, Professional Papers, and Capstones

Endurance running and participation in racing events has flourished over the last decade. Recreational runners enter the training period for an upcoming race at differing levels of fitness and training. As individuals sign-up for the race, they typically follow the commitment with an increase in training load. The increases in training load are accomplished through a combination of increasing mileage, or duration of running, and running intensity through increase velocity. These increases are theoretically being applied in a progressive overload principle, where the musculoskeletal system has time to adapt to the breakdown provided from the previous training session. Progressive overload …

The Influence Of Crossfit Training On Running Mechanics, Sophia D. Bradley

UNLV Theses, Dissertations, Professional Papers, and Capstones

Introduction

40 million Americans run regularly.1 Contrary to many runner’s beliefs, increased strength may be beneficial while not adding body mass. An increase in strength in specific muscles may improve running gait and performance. 9,6 Strength training may also play an important role in preventing common overuse injuries in running 11,12 Depending on the style of strength training, it may also have an enhanced benefit on running economy.4,16

Participation in CrossFit is also widely popular, with an increase in participation of 923% in the past ten years.2 CrossFit is a type of strength training in which most movements performed utilize …

Optimization Of Prosthetic Hands: Utilizing Modularity To Improve Grip Force, Grasp, And Versatility, Jordan William Harris

UNLV Theses, Dissertations, Professional Papers, and Capstones

It has been demonstrated that although many varieties of upper limb prosthetics exist, commercially available prosthetics are outdated and unsatisfactory. Ineffectiveness and limitations have led to some prosthesis wearers having to own multiple devices, whereas others have given up on them entirely. Even though ample research has been conducted to design and test new hand designs, the industry appears to rest in an overall stagnated state.

It was proposed here, that one problem with prosthetic research is an excess of variables involved in testing, and therefore the improper application of the scientific method. It seems that each time a research …

Eye Repair In Xenopus Laevis, Cindy Xuan-Mai Kha

UNLV Theses, Dissertations, Professional Papers, and Capstones

Eye development in vertebrates of complex steps that include specific interactions of the neuroectoderm and overlying head ectoderm. The African clawed frog, Xenopus laevis (X. laevis), has a well-characterize eye developmental pathway and is an established model for eye regeneration research. Additionally, Xenopus frogs have high regenerative abilities to regenerate individual eye tissues such as the retina, lens, and cornea. However, it was previously shown that the removal of the specified eye field during the neurulation stage or an eye during the swimming tadpole stage does not permit an eye to regenerate. Here we will describe a model for investigating …

Hip Adduction: Abduction Ratio Differences Between Collegiate Male And Female Soccer Players, Addie Lucci

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context: Soccer is a high-intensity sport resulting in injuries. Strength ratios have been used to identify areas of weakness and help prevent further injuries. Objective: The purpose of this study was to determine if there was a difference in hip adduction: abduction ratios in collegiate male and female soccer players. The hypothesis was that males will have a lower ratio than females. Design: Cohort Study. Setting: Research Center. Participants: Five male and nine female NCAA Division 1 soccer players were recruited for this study. Intervention: Independent variable is gender. Biodex Isokinetic Dynamometer was used for data collection. The subjects performed …

A Comparison Of Outdoor Bioaerosol And Particulate Measurements In Las Vegas, Tanviben Yogeshkumar Patel

UNLV Theses, Dissertations, Professional Papers, and Capstones

The urbanization of the Las Vegas Valley has turned the desert into a green oasis by introducing many non-native plant species, some of which are allergenic. Outdoor bioaerosols consist of microorganisms, pollen, spores, and other agents that could cause infections or affect the health of humans. Studies have suggested that exposure to bioaerosols through inhalation can lead to various human health risks. Typically, one monitoring station is established per city to obtain pollen and mold counts for an entire metropolitan area, and the site may not be representative of community exposures. The objective of this study was to measure and …

Upper Extremity Muscle Activity During Simulated Dryland Swimming While Wearing Wetsuit, Ciro J. Agnelli

UNLV Theses, Dissertations, Professional Papers, and Capstones

Triathletes typically wear a wetsuit during the swim portion of an event, but it is not clear if muscle activity is influenced by wearing a wetsuit. The purpose of this study was to investigate if shoulder muscle activity was influenced by wearing a full sleeved wetsuit vs. no wetsuit during simulated dryland swimming. Subjects (n=10 males; 179.07±13.18cm; 91.20±7.25 kg; 45.6±10.52 years) completed two dry land simulated swimming conditions (VASA Ergometer Trainer): No Wetsuit (NWS) and with Wetsuit (WS). Electromyography (EMG) of four upper extremity muscles was recorded (Noraxon telemetry EMG, sample rate = 1000 Hz) for each condition: Trapezius (TRAP), …

A Rural And Urban Study Of The Food Environment In Select Nevada Geographies, Joseph Nickelson

UNLV Theses, Dissertations, Professional Papers, and Capstones

National and county level examination of urban and rural food environments has taken place, but primary validation of venue existence and in-venue data are lacking. The literature show disparities in access to healthy foods through low density of large grocers/supermarkets, low variety and quality of produce and higher prices for a healthy diet in rural versus urban geographies. Rural areas are of unique concern due to poor health indicators such as higher obesity rates, higher diabetes rates, lower incomes and lower educations than urban residents. The goal of this study was to explore differences in the community and consumer level …

Stride Leg Ground Reaction Forces Pre- And Post-Fatigue In Collegiate Baseball Pitchers, Courtney Alley

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context: Baseball pitching requires the coordination of the lower and upper extremities to effectively generate and transfer force to the ball. High-velocity pitchers have been found to have significantly greater stride leg force generation than low-velocity pitchers. The influence of stride leg fatigue on pitching has yet to be investigated. Objective: The purpose of this study was to examine the effect of stride leg fatigue on peak vertical ground reaction forces (vGRFs) and hand velocity. Design: Pretest-posttest. Setting: Biomechanics laboratory. Patients or Other Participants: A convenience sample of 11 collegiate baseball pitchers (19.27 ± 0.64 years old; 85.88 ± 12.16 …

An Evaluation Of Gabab Receptors On Modulating Neuroinflammation In A Non-Transgenic Animal Model Of Alzheimer's Disease, Monica Bolton

UNLV Theses, Dissertations, Professional Papers, and Capstones

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by progressive memory loss and distinct neuropathological hallmarks, including amyloid beta plaques and neurofibrillary tau tangles (NFT). Although the etiology remains to be discovered, several risk factors exist that significantly contribute to developing AD. Diabetes is one of the major risk factors associated with AD and is characterized by disrupted insulin signaling that may contribute to or exacerbate AD pathologies. Furthermore, both disorders result in increased neuroinflammation. Considerable evidence has demonstrated that a chronic inflammatory response, in particular chronic microglia activation, promotes A production as well as the hyperphosphorylation of tau through …

Are Vending Machine Selections Healthier? Trends In Dietary Quality Of Vending Machine Food And Beverage Selections Among Nhanes Participants Age 6-19 Years Between 2003-2012, Aurora Maria Buffington

UNLV Theses, Dissertations, Professional Papers, and Capstones

Dietary intake is related to 4 major causes of death and may be influenced by the food environment, which includes the $64.3 billion revenue-producing vending machine industry. Most machines contain low nutrient energy dense foods and beverages associated with poor dietary choices, while healthier vending initiatives are seen as a strategy to increase access to healthy foods. Elementary and secondary schools have increasingly adopted healthier vending standards in response to federal child nutrition regulation and student wellness policy implementation, however an association between vending and diet has not been made using a large sample of nationally representative data. The purpose …

Gene Expression Profiling In The Larval Fat Body Of Desiccation Selected Drosophila Melanogaster, Adriana Michelle Charles

UNLV Theses, Dissertations, Professional Papers, and Capstones

Drosophila melanogaster selected for resistance to desiccation (no food or water) display slower development and a higher body mass compared to fed controls due to an extended third larval instar. I hypothesize that desiccation selected D. melanogaster larvae will have a different gene expression profile compared to fed controls. Separate populations of D. melanogaster were subjected to desiccation (no food or water), starvation (no food) until 80-85% mortality, for 75 generations. mRNA from the larval fat body was collected at 88 hours, 96 hours, 112 hours and 120 hours post-hatching. Four replicate samples were used for each condition and time …

Determining The Effects Of Social Media Monitoring To Identify Potential Foodborne Illness In Southern Nevada, Lauren Diprete

UNLV Theses, Dissertations, Professional Papers, and Capstones

Foodborne illness, commonly referred to as food poisoning, affects an estimated 1 in 6 Americans every year, despite the fact that it is entirely preventable. Many cases of foodborne illness go unreported; however, better reporting leads to faster health department response and containment. Social media monitoring, using software to identify trends in social media posts, is a novel new tool that has been tested in a variety of public health fields with promising preliminary results. The Southern Nevada Health District (SNHD) has employed social media monitoring software to identify potential foodborne illness within Southern Nevada. The purpose of this study …

Aging And Fermentation As Adaptive Food Management Strategies In The Arctic, Celeste Giordano

UNLV Theses, Dissertations, Professional Papers, and Capstones

This dissertation is composed of three papers: one published article, one article under review for publication, and one published commentary. Chapter 1 provides an introduction to the dissertation as a whole – a work that investigates food aging and fermentation techniques in an indigenous Yup’ik Alaskan community, proposes an adaptive explanation for these strategies in the circumpolar north, and explores the potential importance of them cross-culturally and evolutionarily. Chapter 2 is a paper that describes the seal poke storage system – an indigenous food preservation and storage technique that Yup’ik Alaskans used to manage food security up until approximately one …

Factors Influencing Induction Of Desiccation Tolerance In Bryophytes: Redefining Fundamental Aspects Of The Organism’S Relationship With The Environment In Xeric Habitats, Joshua Lee Greenwood

UNLV Theses, Dissertations, Professional Papers, and Capstones

If one wishes to understand the ecological role, range, habitat preferences, selective pressures, reproduction, response to climate change, and the potential for survival under climactic change, range expansion and range contraction for species and populations of bryophytes, an understanding of their survival of the desiccated state, is essential. In order to further our understanding of desiccation tolerance in mosses, three projects were undertaken. The first of these investigated the nature of desiccation tolerance in mosses, specifically if desiccation tolerance can be induced; thereby providing a desiccation tolerant phenotype in a moss species generally considered desiccation sensitive (Physcomitrella patens, the model …

Living On The Edge: Assessing The Effects Of Catastrophic Fire On Plants Utilized By Two Endemic Subspecies Of Spring Mountains Butterflies, Charles Ryan Herrmann

UNLV Theses, Dissertations, Professional Papers, and Capstones

In the past few decades there has been an increase in catastrophic, high-intensity, large-scale wildfires globally due to the combination of climate warming with more than a century of fire suppression policy. One region that has been drastically affected is the Western United States, as there has been an increase in ‘mega-fires’ in the past few decades. The 2013 Carpenter 1 Fire in the Spring Mountains, Nevada was the largest fire in recorded history in these mountains, spreading out over 11,137 hectares. Catastrophic fire like the Carpenter 1 Fire can have potentially devastating effects on endemic species inhabiting refugia on …

Concussion Prevention Strategies: A Survey Of Division I And Division Ii Female Soccer Teams, Kori Kendall Jeffries

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study is to evaluate concussion prevention strategies that are being used in NCAA Division I and Division II schools, perform an investigation of cervical strengthening programs and the concussion rates associated, and determine beliefs of Certified Athletic Trainers about equipment for concussion prevention. Participants included Certified Athletic Trainer’s (ATs) employed at NCAA Division I and Division II universities in the United States, and provide services to the universities’ female soccer team. Data was collected via questionnaire e-mailed to participants through Qualtrics survey software. A prompt was sent out two weeks and four weeks following the initial …

Behavioral Paleoecology Of Lower Cambrian Deposit Foragers: Reinterpreting Looping And Meandering Traces Using Optimal Foraging Theory And Quantitative Analysis, Zachary Andrew Jensen

UNLV Theses, Dissertations, Professional Papers, and Capstones

The early Cambrian was a period of transition during which the seafloor environment was changing from a microbial-mat-dominated environment to a bioturbation-dominated environment. The result was a patchy landscape of variable food resources for foraging burrowers to exploit. Looping trace fossils, such Psammichnites gigas and Taphrhelminthopsis nelsoni, appear in strata worldwide during this transitional period, and the ecological niche they filled is a subject of debate among ichnologists. The objectives of this study are (1) to determine the foraging strategies preserved by looping traces through the application of optimal foraging theory and quantitative analysis, and (2) use those results to …

Thermal And Developmental Ecology Of Pupfish, Cyprinodon, Alexander Jones

UNLV Theses, Dissertations, Professional Papers, and Capstones

Pupfish from the genus Cyprinodon are among the most endangered groups of fishes on the planet, with nearly 40% of species being threatened with extinction. These pupfishes are often assumed to be the most temperature tolerant of all fish, coping with temperatures as low as 0 °C and as high as 44 °C. However, conflicting data exist and certain measures of thermal tolerance are not markedly higher in pupfish compared to common game fish. Pupfish egg production and growth has been known to be hampered at temperatures well below what they apparently experience in nature. I addressed why eggs fail …

Evaluation Of A Fluorescence Method For Quantifying Bioaerosol Concentrations On Air Quality Filter Samples, Rachel Kolberg

UNLV Theses, Dissertations, Professional Papers, and Capstones

Airborne particulate matter (PM) in outdoor environments contains many components that cause adverse human health effects. The size of the particulates determine in what manner the particles would bypass the body’s defense mechanisms to enter the respiratory system and is directly related to their health impacts. Currently the United States Environmental Protection Agency is enforcing the National Ambient Air Quality Standards (NAAQS) to regulate the annual and 24-hour average concentrations of PM2.5 and PM10 in the air. PM2.5 are fine particles with aerodynamic diameter <2.5μm, small enough to reach the deepest parts of the bronchi and lungs. PM10 include PM2.5 and larger particles with aerodynamic diameter of 2.5-10μm. Both PM2.5 and PM10 contain multiple components from multiple sources. Bioaerosols are an important component of PM, but there is limited knowledge about how bioaerosols contribute to PM2.5 and PM10 concentrations. There is also a lack of research about the incidence and prevalence of disease caused by bioaerosols and about the limits of exposure to bioaerosol particulates. The main barrier to assess bioaerosol concentrations and health-related effects is the absence of quick and inexpensive methodology for quantifying bioaerosols. This study explored the feasibility of using fluorescence microscopy to quickly quantify bioaerosols in PM2.5 and PM10 collected on polycarbonate filters. Bioaerosols were stained with a DNA marker directly on a filter, followed by fixation, microscopic imaging, and automatic counting. The method was first validated using reference samples prepared by depositing different known concentrations of E. coli onto blank polycarbonate filters. The results indicated a linear response over two orders of magnitude (R2 = 0.9) and an accuracy within ±25%. E. coli were also deposited onto selected ambient PM10 and PM2.5 filter samples to determine if pre-loaded particles would interfere with bioaerosol imaging and counting. It was found that despite an increase in uncertainty (variability), the calibration slope remained within ±10% of unity for both PM2.5 and PM10 samples. Bioaerosol concentrations in ambient samples, as quantified by this method, were on average 14% higher for PM10 than for PM2.5 acquired concurrently in a desert environment of Las Vegas, Nevada. The application of this method to other types of compliance filters, such as Teflon filters and tapes of a Beta Attenuation Monitor (BAM) were also explored in this study. By means of a high-yield approach this method is expected to facilitate bioaerosol research, support exposure and health assessments, and help refine NAAQS for PM2.5 and PM10.

The Psychophysiological Correlates Of Personality, Trauma, Posttraumatic Stress Disorder And Social Support, Meghan E. Pierce

UNLV Theses, Dissertations, Professional Papers, and Capstones

Theories considering the etiology of psychopathy suggest that trauma exposure, specifically childhood maltreatment and sexual abuse, is related to the development of callous-unemotional traits in children and adolescents, which are precursors to psychopathic traits in adulthood. Furthermore, posttraumatic stress disorder has an opposite relationship with many of the emotional and behavioral components of the two-factor model of psychopathy. Specifically, PTSD is positively associated to IA and traits associated with it and negatively associated with FD. Thus, this study sought to expand upon the current theories of a trauma-based etiology of psychopathy by investigating the relationship between trauma, PTSD, and psychopathic …

The Effects Of An Acute Bout Of Exercise On Hunger Hormones In Individuals At Risk For Type 2 Diabetes, Sydney Spoon

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background: Hunger hormone levels are typically dysregulated in obese and diabetic

populations, however; postprandial exercise has been shown to influence hunger hormone levels. Purpose: To determine if hunger hormones including acylated ghrelin and GLP-1 levels are altered in response to an acute bout (15 minutes) of walking in individuals at risk for type 2 diabetes mellitus (T2DM).

Methods: Ten subjects at risk for prediabetes (fasting blood glucose 100–125mg/dL) participated in this randomized crossover design trial. Subjects arrived at the laboratory following an overnight fast and underwent one of two conditions: 1) Test meal with no walking (CON) or 2) Test …