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Full-Text Articles in Life Sciences
Co-Transcriptomic Analysis Of The Maize–Western Corn Rootworm Interaction, Lise Pingault, Saumik Basu, Neetha N. Vellichirammal, William Paul Williams, Gautam Sarath, Joe Louis
Co-Transcriptomic Analysis Of The Maize–Western Corn Rootworm Interaction, Lise Pingault, Saumik Basu, Neetha N. Vellichirammal, William Paul Williams, Gautam Sarath, Joe Louis
Department of Entomology: Faculty Publications
The Western corn rootworm (WCR; Diabrotica virgifera virgifera) is an economically important belowground pest of maize. Belowground feeding by WCR is damaging because it weakens the roots system, diminishes nutrient uptake, and creates entry points for fungal and bacterial pathogens and increases lodging, all of which can significantly suppress maize yields. Previously, it was demonstrated that belowground herbivory can trigger plant defense responses in the roots and the shoots, thereby impacting intraplant communication. Although several aspects of maize-WCR interactions have been reported, co-transcriptomic remodeling in the plant and insect are yet to be explored. We used a maize genotype, …
Wheat Streak Mosaic Virus Alters The Transcriptome Of Its Vector, Wheat Curl Mite (Aceria Tosichella Keifer), To Enhance Mite Development And Population Expansion, Adarsh K. Gupta, Erin D. Scully, Nathan A. Palmer, Scott M. Geib, Gautam Sarath, Gary L. Hein, Satyanarayana Tatineni
Wheat Streak Mosaic Virus Alters The Transcriptome Of Its Vector, Wheat Curl Mite (Aceria Tosichella Keifer), To Enhance Mite Development And Population Expansion, Adarsh K. Gupta, Erin D. Scully, Nathan A. Palmer, Scott M. Geib, Gautam Sarath, Gary L. Hein, Satyanarayana Tatineni
Department of Entomology: Faculty Publications
Wheat streak mosaic virus (WSMV; genus Tritimovirus; family Potyviridae) is an economically important wheat virus that is transmitted by the wheat curl mite (WCM; Aceria tosichella Keifer) in a persistent manner. Virus–vector coevolution may potentially influence vector gene expression to prolong viral association and thus increase virus transmission efficiency and spread. To understand the transcriptomic responses of WCM to WSMV, RNA sequencing was performed to assemble and analyse transcriptomes of WSMV viruliferous and aviruliferous mites. Among 7291 de novo-assembled unigenes, 1020 were differentially expressed between viruliferous and aviruliferous WCMs using edgeR at a false discovery rate ≤0.05. …
Transcriptional Profiling Of Resistant And Susceptible Buffalograsses In Response To Blissus Occiduus (Hemiptera: Blissidae) Feeding, Crystal M Ramm, Michael Wachholtz, Keenan Amunsen, Teresa J. Donze, Tiffany Heng-Moss, Paul Twigg, Nathan A. Palmer, Gautam Sarath, Frederick P. Baxendale
Transcriptional Profiling Of Resistant And Susceptible Buffalograsses In Response To Blissus Occiduus (Hemiptera: Blissidae) Feeding, Crystal M Ramm, Michael Wachholtz, Keenan Amunsen, Teresa J. Donze, Tiffany Heng-Moss, Paul Twigg, Nathan A. Palmer, Gautam Sarath, Frederick P. Baxendale
Department of Entomology: Faculty Publications
ABSTRACT Understanding plant resistance mechanisms at a molecular level would provide valuable insights into the biological pathways impacted by insect feeding, and help explain specific plant tolerance mechanisms. As a first step in this process, we conducted next-generation sequencing using RNA extracted from chinch bug-tolerant and -susceptible buffalograss genotypes at 7 and 14 d after chinch bug feeding. Sequence descriptions and gene ontology terms were assigned to 1,701 differentially expressed genes. Defense-related transcripts were differentially expressed within the chinch bug-tolerant buffalograss, Prestige, and susceptible buffalograss, 378. Interestingly, four peroxidase transcripts had higher basal expression in tolerant control plants compared with …
High-Throughput Transcriptome Sequencing For Snp And Gene Discovery In A Moth, Nicholas J. Miller, Jing Sun, Thomas W. Sappington
High-Throughput Transcriptome Sequencing For Snp And Gene Discovery In A Moth, Nicholas J. Miller, Jing Sun, Thomas W. Sappington
Department of Entomology: Faculty Publications
The western bean cutworm, Striacosta albicosta (Smith) (Leptidoptera: Noctuidae) is a pest of corn (Zea mays L.) and dry beans that underwent a dramatic range expansion in North America during the first decade of the 21st century. Research into the population genetics of this species has been hindered by a lack of genetic markers. The transcriptome of adult male S. albicosta was partially sequenced using Illumina sequencing-by-synthesis. Assembly of the sequence reads yielded 16,847 transcript sequences, of which 6,631 could be assigned a putative function. A search for single nucleotide polymorphisms (SNPs) identified 2,487 candidate SNPs distributed among 1,265 …