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Full-Text Articles in Life Sciences

Neotype Designation For Papilio Fulgerator Walch, 1775 (Hesperiidae: Eudaminae), Jing Zhang, Qian Cong, Gerardo Lamas, Nick V. Grishin Nov 2022

Neotype Designation For Papilio Fulgerator Walch, 1775 (Hesperiidae: Eudaminae), Jing Zhang, Qian Cong, Gerardo Lamas, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

The discovery that a skipper butterfly Telegonus fulgerator (Walch, 1775), previously placed in the genus Astraptes Hübner, [1819], is a complex of many similar-looking species-level taxa with different COI barcodes, caterpillar foodplants, and body patterns, and subtle differences in adult phenotypes raised a question about which species is the original T. fulgerator. To answer this question, being unable to locate its holotype, we designate the neotype of Papilio fulgerator Walch, 1775, a female specimen from Suriname in the Zoological State Collection, Munich, Germany. This neotype will form the foundation for a comprehensive revision of the T. fulgerator complex based …


Genomics And Transcriptomics To Protect Rice (Oryza Sativa L.) From Abiotic Stressors: -Pathways To Achieving Zero Hunger, Mushtaq Ahmad Oct 2022

Genomics And Transcriptomics To Protect Rice (Oryza Sativa L.) From Abiotic Stressors: -Pathways To Achieving Zero Hunger, Mushtaq Ahmad

Center for Plant Science Innovation: Faculty and Staff Publications

More over half of the world’s population depends on rice as a major food crop. Rice (Oryza sativa L.) is vulnerable to abiotic challenges including drought, cold, and salinity since it grown in semi-aquatic, tropical, or subtropical settings. Abiotic stress resistance has bred into rice plants since the earliest rice cultivation techniques. Prior to the discovery of the genome, abiotic stressrelated genes were identified using forward genetic methods, and abiotic stress-tolerant lines have developed using traditional breeding methods. Dynamic transcriptome expression represents the degree of gene expression in a specific cell, tissue, or organ of an individual organism at …


Taxonomic Discoveries Enabled By Genomic Analysis Of Butterflies, Jing Zhang, Qing Cong, Jinhui Shen, Leina Song, Riley J. Gott, Pierre Boyer, Crispin S. Guppy, Steve Kohler, Gerardo Lamas, Paul A. Opler, Nick V. Grishin Oct 2022

Taxonomic Discoveries Enabled By Genomic Analysis Of Butterflies, Jing Zhang, Qing Cong, Jinhui Shen, Leina Song, Riley J. Gott, Pierre Boyer, Crispin S. Guppy, Steve Kohler, Gerardo Lamas, Paul A. Opler, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

The comparative genomics of butterflies yields additional insights into their phylogeny and classification that are compiled here. As a result, 3 genera, 5 subgenera, 5 species, and 3 subspecies are proposed as new, that is, in Hesperiidae: Antina Grishin, gen. n. (type species Antigonus minor O. Mielke, 1980), Pompe Grishin and Lamas, gen. n. (type species Lerema postpuncta Draudt, 1923), and Curva Grishin, gen. n. (type species Moeris hyagnis Godman, 1900); in Lycaenidae: Fussia Grishin, subgen. n. (type species Polyommatus standfussi Grum-Grshimailo, 1891) and Pava Grishin, subgen. n. (type species Thecla panava Westwood, 1852); in Hesperiidae: Monoca Grishin, subgen. n. …


Hybrid Incompatibility Between Ldrosophila Virilis And D. Lummei Is Stronger In The Presence Of Transposable Elements, Dean M. Castillo, Leonie C. Moyle Aug 2022

Hybrid Incompatibility Between Ldrosophila Virilis And D. Lummei Is Stronger In The Presence Of Transposable Elements, Dean M. Castillo, Leonie C. Moyle

Nebraska Extension: Faculty and Staff Publications

Mismatches between parental genomes in selfish elements are frequently hypothesized to underlie hybrid dysfunction and drive speciation. However, because the genetic basis of most hybrid incompatibilities is unknown, testing the contribution of selfish elements to reproductive isolation is difficult. Here, we evaluated the role of transposable elements (TEs) in hybrid incompatibilities between Drosophila virilis and D. lummei by experimentally comparing hybrid incompatibility in a cross where active TEs are present in D. virilis (TE+) and absent in D. lummei, to a cross where these TEs are absent from both D. virilis (TE−) and D. lummei genotypes. Using genomic data, …


Checking The Checkered Taxonomy Of Plötz's Checkered Skippers (Hesperiidae: Pyrgini), Jing Zhang, Qing Cong, John M. Burns, Nick V. Grishin May 2022

Checking The Checkered Taxonomy Of Plötz's Checkered Skippers (Hesperiidae: Pyrgini), Jing Zhang, Qing Cong, John M. Burns, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

We present an analysis of the names proposed by Carl Plötz in 1884 for the New World species in the genus Pyrgus Hübner, [1819] facilitated by the genomic sequencing of extant primary type specimens comparatively with a larger sample of more recently collected specimens of these species and their relatives. The changes to nomenclature suggested here are only caused by the identity of primary type specimens as revealed by their phenotypes or though genomic sequencing. All neotypes are designated to stabilize nomenclature in agreement with the current usage of these names, which in unison agrees best with the information available …


Genomic Dna Sequencing Reveals Two New North American Species Of Staphylus (Hesperiidae: Pyrginae: Carcharodini), Jing Zhang May 2022

Genomic Dna Sequencing Reveals Two New North American Species Of Staphylus (Hesperiidae: Pyrginae: Carcharodini), Jing Zhang

The Taxonomic Report of the International Lepidoptera Survey

Two new skipper butterfly (Hesperiidae) species are described from the United States: Staphylus floridus Grishin, sp. n. (type locality in Florida, Volusia County) and Staphylus ecos Grishin, sp. n. (type locality in Texas, Brewster County). They are cryptic and hence escaped recognition. They differ from their sister species by the relative size and morphology of genitalia and by genotype—including and beyond the COI barcode—thus, suggesting genetic isolation that argues for their species-level status. A lectotype is designated for Helias ascalaphus Staudinger, 1876. Staphylus opites (Godman & Salvin, 1896), stat. rest. is a species-level taxon and not a synonym of Staphylus …


Annotating Gene Expression And Regulatory Elements In Tissues From Healthy Thoroughbred Horses And Identifying Candidate Mutations Associated With Perosomus Elumbis In An Angus Calf, Alexa Barber Apr 2022

Annotating Gene Expression And Regulatory Elements In Tissues From Healthy Thoroughbred Horses And Identifying Candidate Mutations Associated With Perosomus Elumbis In An Angus Calf, Alexa Barber

Department of Animal Science: Dissertations, Theses, and Student Research

Genome annotation has a direct impact on the success of genomic studies. Transcriptome analyses and chromatin immunoprecipitation and sequencing (ChIP-seq) have been used to functionally annotate genomes. These methods can identify protein-coding genes, non-coding transcripts, and cis-regulatory elements across the genome. The primary objective of the first study was to functionally annotate the equine genome through the assessment of nine tissues: adipose, brain, heart, lamina, liver, lung, skeletal, muscle, testis, and ovary. In the first project, 150 bp, paired-end RNA sequencing (RNA-seq) libraries were generated in stallion tissues and compared to previously generated mare RNA-seq libraries to quantify variation in …


Editorial: Genomics-Enabled Triticeae Improvement, Xue-Feng Ma, Xianchun Xia, Shuyu Liu, P. Stephen Baenziger, Hakan Özkan Mar 2022

Editorial: Genomics-Enabled Triticeae Improvement, Xue-Feng Ma, Xianchun Xia, Shuyu Liu, P. Stephen Baenziger, Hakan Özkan

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.


Taxonomic Changes Suggested By The Genomic Analysis Of Hesperiidae (Lepidoptera), Jing Zhang, Qian Cong, Jinhui Shen, Nick V. Grishin Feb 2022

Taxonomic Changes Suggested By The Genomic Analysis Of Hesperiidae (Lepidoptera), Jing Zhang, Qian Cong, Jinhui Shen, Nick V. Grishin

Insecta Mundi

Our expanded efforts in genomic sequencing to cover additional skipper butterfly (Lepidoptera: Hesperiidae) species and populations, including primary type specimens, call for taxonomic changes to re­store monophyly and correct misidentifications by moving taxa between genera and proposing new names. Reconciliation between phenotypic characters and genomic trees suggests three new tribes, two new sub­tribes, 23 new genera, 17 new subgenera and 10 new species that are proposed here: Psolosini Grishin, new tribe (type genus Psolos Staudinger, 1889), Ismini Grishin, new tribe (type genus Isma Distant, 1886), Eetio­nini Grishin, new tribe (type genus Eetion de Nicéville, 1895), Orphina Grishin, new subtribe (type …