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Full-Text Articles in Life Sciences
Identification Of Soybean Seed Oil Qtls With Little Or No Impact On Seed Protein, Yu-Kai Sun
Identification Of Soybean Seed Oil Qtls With Little Or No Impact On Seed Protein, Yu-Kai Sun
Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research
A QTL (Quantitative Trait Locus) is chromosomal location of a gene controlling a specific phenotypic characteristic (trait). This trait might be governed by two or more genes and may be affected by environmental interaction. The USA soybean seed composition, when averaged over years and states, is 18.7% oil and 35.3% protein. Soybean seed provides cooking oil for humans and protein for livestock. Concurrent genetic improvement of seed protein (pro) and oil content has been difficult to achieve due to the negative genetic correlation of the two traits. This negative correlation could be due to a pair of tightly linked protein …
Mutational Analysis Of The Major Soybean Uref Paralogue Involved In Urease Activation, Joe C. Polacco, D. L. Hyten, Mônica Medeiros-Silva, David A. Sleper, Kristin D. Bilyeu
Mutational Analysis Of The Major Soybean Uref Paralogue Involved In Urease Activation, Joe C. Polacco, D. L. Hyten, Mônica Medeiros-Silva, David A. Sleper, Kristin D. Bilyeu
Department of Agronomy and Horticulture: Faculty Publications
The soybean genome duplicated ~14 and 45 million years ago and has many paralogous genes, including those in urease activation (emplacement of Ni and CO2 in the active site). Activation requires the UreD and UreF proteins, each encoded by two paralogues. UreG, a third essential activation protein, is encoded by the single-copy Eu3, and eu3 mutants lack activity of both urease isozymes. eu2 has the same urease-negative phenotype, consistent with Eu2 being a single-copy gene, possibly encoding a Ni carrier. Unexpectedly, two eu2 alleles co-segregated with missense mutations in the chromosome 2 UreF paralogue (Ch02UreF), suggesting …