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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
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Biotinylation Of K12 In Histone H4 Decreases In Response To Dna Double-Strand Breaks In Human Jar Choriocarcinoma Cells1,2, Nagarama Kothapalli, Gautam Sarath, Janos Zempleni
Biotinylation Of K12 In Histone H4 Decreases In Response To Dna Double-Strand Breaks In Human Jar Choriocarcinoma Cells1,2, Nagarama Kothapalli, Gautam Sarath, Janos Zempleni
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
We tested the hypothesis that biotinylation of K12 in histone H4 plays a role in the cellular response to double-strand breaks (DSB) of DNA in human cells. DSB were caused by treating choriocarcinoma JAr cells with etoposide. Biotinylation of K12 in histone H4 decreased by 50% as early as 10–20 min after initiation of treatment with etoposide. Biotinylation returned to initial levels 30–40 min after the addition of etoposide to the medium. Temporal patterns of K12-biotinylation were similar for human lymphoma cells. Phosphorylation of S14 of histone H2B and poly(ADP-ribosylation) of glutamate residues on histone H2A are known markers of …