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A Modified Molybdenum Blue Method For Orthophosphate Determination Suitable For Investigating Enzymatic Hydrolysis Of Organic Phosphates, Zhongqi He, C. Wayne Honeycutt
A Modified Molybdenum Blue Method For Orthophosphate Determination Suitable For Investigating Enzymatic Hydrolysis Of Organic Phosphates, Zhongqi He, C. Wayne Honeycutt
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
In characterizing organic phosphorus (Po) by phosphatase hydrolysis, the quantity of hydrolyzable Po is represented by the difference in orthophosphate [i.e., inorganic P (Pi)] determined after and prior to enzymatic incubation. Therefore, precise determination of Pi is of major importance for accurate application of the enzymatic hydrolysis approach. The strong acid conditions required for conventional molybdenum blue methods interferes with Pi determination due to rapid hydrolysis of labile Po and precipitation of enzymes (proteins). The molybdenum blue method of Dick and Tabatabai in 1977 reduced errors pertaining to nonenzymatic hydrolysis of P …