Life Sciences Commons^™

Sew Zambia! Home Economics In Primary & Secondary Schools Of The Eastern & Southern Provinces, Clare Schinzel

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Introduction: Home Economics (HE) is within the main curriculum for primary school students in Zambia, yet it remains unclear what is included.

Methods: Survey research conducted between May-June 2022 on 6-7th-grade students within 9 Zambian schools. Classroom observations were made, and teachers were interviewed in primary and secondary public schools.

Results: Most know how to cook and sew. Zambian women cook more than men and learn at an older age. Both males and females are trained to sew learning at a much younger age.

Discussion: HE remains critical to learning life skills in Zambia and the curriculum reflects skills related …

Investigation Of Rapid Diagnostic Tests For Characterization Of Mycobacterium Avium Complex (Mac) From Various Isolates And Identification Of Virulence Factors Of Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) In Vitro, Claudia Antonika

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Chapter 1

Mycobacterium avium complex (MAC) has become one of the major problems in public health and livestock. Members of MAC, such as M. avium subsp paratuberculosis (MAP) and M. avium subsp hominissuis (MAH), are responsible for many opportunistic infections and the loss of livestock. MAP is economically significant to the beef and dairy industries because it is the etiologic agent of Johnes’s disease, a chronic and fatal enteritis in ruminants. Tracing the infection sources of MAC could be difficult since it infects many types of hosts in the natural environment. Furthermore, there is less information known about MAP pathogenicity …

The Effects Of Phosphate On Larval Western Barred Tiger Salamanders (Ambystoma Mavortium), Lexy Polivanov, Dennis Ferraro

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This study seeks examine the effects of elevated phosphate levels on the larval stage of the Western Barred Tiger Salamander (Ambystoma mavortium). Hypothesis: Additional phosphate in the water will cause the larval salamanders to have a slower metamorphosis rate than the control group with no additional phosphates. This hypothesis is based off of research that showed that additional nitrogen in the water caused a slower growth rate in the Western Barred Tiger Salamander (Ambystoma mavortium) (Griffis-Kyle, 2007).

The hypothesis was rejected. The dates the larvae morphed alternated between treatment and control. The treatment metamorphosis was not …

Remediation Strategies To Reduce Heavy Metal Uptake In Lettuce Grown In Contaminated Urban Soil, Ryley Thomas

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Urban soils are increasingly used to produce food for local consumption, which requires at the same time management strategies that prevent the plant uptake of potentially present contaminants. This study was conducted to test different soil amendments for their ability to retain lead (Pb) and arsenic (As) within the soil matrix. The analyzed soil was taken from a potential community garden lot near a railroad in Lincoln, NE where elevated concentrations in Pb and As had been detected. Ponderosa pine biochar and spent coffee grounds were used as soil additives because of their documented chemical reactivity towards soil cations or …

Developing A Method To Contain The Feeding Of The Wheat Curl Mite (Aceria Tosichella Keifer), Pierce Leef, Gary Hein

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To better understand the ecology and epidemiology of the wheat curl mite (Aceria tosichella Keifer) as a vector of wheat streak mosaic virus (WSMV), it is necessary to develop a method to confine the microscopic mites and their infection to specific areas of their host plants, particularly wheat and corn. In this study, corn was the plant of interest. The device developed for this mite-confining method was made by removing the lens of a phone camera lens clip and gluing a 2.5 cm x 4.5 cm piece of wood to the other arm of the clip. Clips were utilized by …

Fungicide Sensitivity Of Sclerotinia Sclerotiorum Isolates From Five States With Different Fungicide Treatments, Cristian Wulkop Gil, Edgar Nieto-Lopez, Sydney Everhart

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Sclerotinia sclerotiorum is a plant pathogenic fungus that causes a disease called white mold that can infect more than 450 plant species including soybeans, dry beans, green beans, canola, and sunflower. This pathogen is capable of up to $252M in losses every year (U.S. Canola Association, 2014). Fungicides are widely used in developed agricultural systems to control disease. However, resistance to the most effective fungicides has emerged and spread in pathogen populations and there have been multiple reports of S. sclerotiorum isolates becoming resistant to certain fungicides. Since different fields in different states use different fungicide treatments on plants and …

Zika Virus Induces Apoptosis In Retinal Pigmented Epithelial Cells, Madison Kraus, Philma Glora Muthuraj, Sathish Kumar Natarajan

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Zika virus is a single-stranded positive sense RNA virus that attracted international attention in 2015 when an epidemic broke out in Brazil. This vector borne virus is transmitted by mosquitos and is especially concerning for pregnant women because the virus can affect the development of the fetus. One known complication is the destruction of infected retinal epithelial cells. The transduction of light is interrupted due to cell death and results in blind spots. Previous research has demonstrated that palmitoleate can reduce the observed levels of apoptosis in ZIKAV infected JEG-3 cells. We have also seen the protection of retinal pigmented …

Evolutionary And Ecological Determinism Of Host Specificity In Arthropod Parasites, Joseph Levey

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Understanding why some diseases infect more species than others is crucial for predicting where and when disease will spread, which can inform the management of wildlife, agriculture, and human health. Currently, large scale patterns of host-parasite dynamics are being studied to understand where to look for and how to manage emerging human diseases (Leroy 2005; Benelli 2018). Previous research has used the Global Mammal Parasite Database (GMPD) to look at host breadth—the number and diversity of species a pathogen can infect—for various groups of parasites, e.g. helminths, arthropods, fungi, etc., from a host-centric perspective (Stephens et al. 2017; Park et …

Do Lactic Acid Bacteria In Fermented Foods Persist In The Gastrointestinal Tract: An In Vitro Investigation, Chloe M. Christensen, Car Reen Kok, Robert W. Hutkins

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It is now well established that the composition of the gastrointestinal (GI) microbiota has a profound influence on intestinal and overall health. Many contemporary diseases such as type two diabetes, IBS and obesity are associated with a dysbiotic microbiota. Therefore, researchers are interested in how diet and specific dietary components can modulate the gastrointestinal microbiota and possibly repair a dysbiotic state. One approach to improve gastrointestinal health is by consuming probiotics and prebiotics or a diet rich in fermented foods containing live microbes. Fermented foods are popular because of their enhanced preservation, safety, organoleptic, functionality, and nutritional properties. These fermented …

Mutagenesis Of Claudins To Probe In Vivo Interactions And Assemblies, Currey Zalman, Alex J. Vecchio

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Paracellular transport of solutes and the control of the flow of molecules through the intracellular space in vertebrate epithelia is directed by tight junctions (TJs). Claudins form paracellular barriers and pores that determine tight junction permeability. This investigation attempts to explain the molecular bases for destruction and reconstruction of tight junctions within epithelial cells, occurring via both natural and disease-causing mechanisms. This interdisciplinary research is important in the advancement of our understanding of human biology and health, as different disruptions to epithelial tight junctions are hallmarks of many human diseases. The overall objective was to take previously made Claudin containing …

What Is The Winter Limiting Nutrient In Urban Reservoirs? A Case Study., Precious Nyabami

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The importance of reservoirs is widely acknowledged by urban populations, yet little is understood scientifically about their ability to process nutrients deposited into them in winter. For example, when agricultural runoff is deposited in lakes in winter or any other season, eutrophication might arise in the spring. With increased eutrophication, several ecosystem services are lost and this leads to what several researchers call “the urban syndrome”. Some studies have been done on the winter limnology of lakes, yet little is understood about the above in reservoirs. The sustainability of urban reservoirs relies on understanding how the phytoplankton in urban reservoirs …

No Association Between An Oxytocin Receptor Genetic Variant And Depressive Symptoms, Rachel Sisley, Grace Sullivan, Scott Stoltenberg Ph. D.

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• Depression has the greatest impact on daily functioning capability of all diseases and adversely effects individuals globally (Flint & Kendler, 2014). • Human capital value of these losses has been about $40 billion dollars annually (Kessler, 2012). • Analysis of the genetic and biological systems associated with depressive symptoms, such as the oxytocin system, could lead to identifying risk variants and possible treatment development. • Genetic Variation in OXTR is associated with a variation in depressive symptoms including low selfesteem, pessimism, and low self-efficacy, etc. (Conner et al., 2018). • The A allele of the SNP rs53576 is considered …

Effect Of Calcium Supplement On Eyesight Of Western Tiger Salamanders, Abigail Horner, Dennis Ferraro

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There has been some discussion in herpetoculture about adding calcium supplements to the diets of salamanders. Adding calcium supplements to a salamander’s diet has been indicated to contribute to blindness. Calcium supplements are usually given to reptiles and amphibians to prevent dietary deficiencies, particularly metabolic bone disease. The link between lack of calcium and metabolic bone disease is well established in reptiles, but is lacking significant research with regards to amphibians. Within the eye of a salamander, there are calcium channels that mediate photosynthetic rods in the absence of light. With a calcium rich diet, it is believed that these …

Management Effects On Soil Quality Of The Geary Soil Series In Thayer County, Nebraska, Aime Nishimwe

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The CGIAR reports that around 40% of global crop yield variability is explained by climate. While global food consumption is expected to soar in 2050, the impact of climate change accounts for approximately 5% decrease in crop productivity. FAO estimates a 60% increase in global food demand by 2050, with global population reaching 9.8 billions. Sustainable soil management was also noted to boost agricultural food production by 58%. The evaluation of the impact of different management practices on dynamic soil properties was conducted on Geary silty clay loam, 3 to 7 %, eroded soils, which are mainly used for crop …

Clostridium Perfringens Adhesion Through Type Iv Pili, Alexander Meyer, Josephine Liess, Kurt Piepenbrink

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Bacteria infect host cells through several mechanisms, one of which is a surface protein called a type IV pilus. Type IV pili are thin, hair-like protein fibers on the bacterial surface capable of being extended and retracted from a bacterial cell; they are used for functions including motility, biofilm formation, and adherence to host cells. Clostridium perfringens is a Gram-positive bacterium best known as a food pathogen that is believed to use type IV pili for host cell adherence. These pili are poorly characterized and understanding how C. perfringens uses type IV pili will provide a model for how these …

Edible Films Made Of Corn Zein Protein And Cellulose Derivatives, Gloria Dukuzeyesu, Changmou Xu, Zhang Yue

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A composite edible film made of corn zein protein (Z) and cellulose derivatives (C) was successfully fabricated. Edible films are coatings used to wrap food products to protect them against microbial deterioration, loss of moisture, and to extend the shelf life of the product. These edible films are consumed together with the product . The mechanical properties, water vapor permeability, application on fruits, and shelf life of the composite film were studied. Zein demonstrated excellent packing characteristics; due to its high no polar contents and hydrophobic nature, zein film served as a moisture barrier. Hydroproxy methylcellulose (HPM) is tasteless, odorless …

Evaluating Larval Movement And Infestation Of Soybean Gall Midge, Genereuse Turabawe, Débora Goulart Montezano, Anthony J. Mcmechan

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Soybean gall midge (SGM) Resseliellamaxima Gagnéis a newly identified insect pest of soybean (Gagnéet al 2019). As of August, 2019 a total of 92 counties across five midwestern states (Fig. 1) documented as infested. Heavily injured soybean fields show visible signs of dead or dying plants (Fig. 2a), causing significant economic losses for farmers.

Heavily injured soybean plants often die prematurely with several different larval stages (Fig. 2b) still feeding within the plant. Significant concern have been raised about the role of these larvae in infesting adjacent healthy plants.

To better understand the behavior and potential for larval infestation a …

Examining Phenotypic Structural Traits As Indicators For Reproductive Longevity Success In Sows, Lindsay Kate Peters, Melanie Dawn Trenhaile-Grannemann, Benny Edd Mote

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The objective of this study was to examine structure and body conformation traits of 494 gilts/sows as a prediction of reproductive longevity in sows. This study focused on specific traits including body length, body depth, front and rear pastern angles, knee angle, and hock angle. The primary method of data collection was based on videos. Still images were pulled from the videos when the sow was standing in a natural stance. The images were analyzed and interpreted objectively with Image J which gives the availability to measure distance and angles given known references in the images. The structural data was …

Microbiota Accessible Carbohydrates And Susceptibility To Clostridioides Difficile Infection, Keegan Schuchart, Jennifer Auchtung, Thomas Auchtung

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This experiment looked into the effects of dietary microbiota accessible carbohydrates (MACs)on the composition of human GI microbe colonies and their susceptibility to pathogen infection. Three different concentrations of MAC ingrained media were tested in mini bioreactor arrays, treated with antibiotics, infected with Clostridioides difficile, then allowed to recover. Microbe community was examined for strain composition and diversity before and after the antibiotic treatment and Clostridioides difficile infection. After the initial mini bioreactor run, individual isolates were investigated in their ability to respond to specific MACs Arabinoglactina, Inulin, and Soluble starch at experimental concentrations.

Fungicide Sensitivity Of Sclerotinia Sclerotiorum Isolates Selected From Five Different States That Use Different Fungicide Treatments, Cristian Wulkop Gil

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Sclerotinia sclerotiorum is a plant pathogenic fungus that causes a disease called white mold that can infect more than 450 plant species including soybeans, dry beans, green beans, canola, and sunflower. This pathogen is capable of up to $252M in losses every year (U.S. Canola Association, 2014). Fungicides are widely used in developed agricultural systems to control disease. However, resistance to the most effective fungicides has emerged and spread in pathogen populations and there have been multiple reports of S. sclerotiorum isolates becoming resistant to certain fungicides. Since different fields in different states use different fungicide treatments on plants and …

Using Hydrogeophysics & Xrf To Produce A High-Resolution 3-Dimensional Soil Cadmium Map For Evaluating Hybrid Wheat Trials, Thierry Bienvenu, Trenton Franz, Associate Professor Of Hydrogeophysics, Nuwan Wijewardane, Post Doc Research Associate, Yufeng Ge, Associate Professor

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Cadmium (Cd) accumulation in wheat decreases germination, growth, grain yield, and in higher concentration leads to adverse effects on human health (Liu et al, 2018). Due to wheat cultivars variation in Cd accumulation, wheat breeders aim to select those at low Cd concentration lines in a field. Hence the need to quantify the concentration of Cd at different parts of a field and visually represent on a high resolution Cd distribution map. Various ways to quantify the concentration of soil Cd exist. However, the cost of equipment required make the process quite expensive and labor intensive. This work studied the …

The Impact Of Nicotine Accumulation Exposure On Lithobates Catebeianus Larvae Mortality, Luke Micek

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Cigarette butts are the most littered item in the world and contain over 4,000 chemicals, including the organic compound nicotine (Slaughter et al. 2011). Billions of cigarette butts are littered each year which may wash into bodies of water, impacting the local wildlife. To determine how introduced chemicals impact the environment, it is important to study its effects on indicator species. Amphibians, such as Lithobates catebeianus, act as indicator species due to their extreme sensitivity to chemical changes in its environment. The purpose of this research project was to obtain data to help determine the impact nicotine accumulation has …

Conservation Of Bta-Mir-29b And Its Effect On Human Gene Expression, Anna Barent, Katie Meinders, Daniel Gutzmann, Audrey L. Atkin

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The purposeof this work was to examine conservation of miR-29b and its binding sites in target mRNAs as well as co-expression of bta-miR-29b in bovine milk exosomes and its target mRNAs in HEK293 cells

•miRNAs are often highly conserved across species and bind to target mRNAs resulting in decreased gene expression. •Bovine milk exosomes contain miRNAs and most of these miRNAs are identical to human miRNAs. •Because these miRNAs are so conserved, it is hypothesized that bovine miRNAs can regulate gene expression and be linked to disease risk.

•miR-29b is highly conserved. •Identified 17 experimentally validated miR-29b target mRNAs. •The …

Mitochondria As A Potential Antifungal Target For Isocyanide Compounds, Nancy Nguyen, Virendra Tiwari, Medhanjali Dasgupta, David Berkowitz, Mark A. Wilson, Wayne R. Riekhof

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The discovery of antibiotics and antifungals greatly impacted medicine and human health, allowing the effective treatment of infections that were previously deadly. However, due to routine and sometimes excessive usage of these compounds, the development of antimicrobial resistance has created a need for new antibiotic and antifungal compounds. Isocyanide compounds have been shown to have antibacterial, antifungal, and anti-cancer properties, but very little is known about their biochemical effects. Our research aims to understand the mechanism of action of isocyanide compounds. We have conducted a genetic screen of a Saccharomyces gene-deletion (“knockout”) collection on media containing an easily synthesized model …

Transect Survey Biases And Correction Methods In Southern Africa, Erika Swenson, Larkin Powell

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In southern Africa, transect surveys and distance-based analyses are often used to obtain density and population estimates for species in large reserves or management zones. However, these estimates may be biased by unnaturally large concentrations of animals at waterholes that are on or near the path of the transect. We used empirical survey data from the Namibrand Nature Reserve in southwest Namibia to parameterize spatial simulations in which we distributed gemsbok (Oryx gazella) on a grid along a 50-kilometer transect. We created multiple simulations with and without waterholes to determine how the proportion of animals clumped at the …

Assembly And Identification Of Novel Viruses In The Aquatic Invertebrate Daphnia Magna, Freddy L. Gonzalez, Reilly O. Cooper, Clayton E. Cressler

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Microbiome studies have been critical to understanding host health and life history. Many studies primarily focus on understanding the impacts of bacteria on the host, while fewer examine the impacts of viruses on host fitness or the interaction between viruses and bacteria within a host. Here, we utilized shotgun sequencing to identify whether viruses were present in the model organism Daphnia magna. VIBRANT, a bioinformatic tool that utilizes hybrid machine learning and protein similarity to identify lytic genomes and other novel viruses, was used on sequencing data from four samples of adult and juvenile Daphnia. We found a …

Creation Of An Influenza B Epigraph Vaccine, Leigh Jahnke, Kristine Hoagstrom, Eric A. Weaver

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The CDC struggles to predict the upcoming influenza viruses resulting in an ineffective influenza vaccine. The 2019 – 2020 influenza vaccine provides an example of vaccine mismatch where the vaccine poorly matches the circulating influenza strains. We have created Influenza B Epigraph hemagglutinin (HA) immunogens that are computationally designed to select the greatest coverage of B and T cell epitopes in the natural population. Our preliminary data shows Epigraph immunogens induce superior cross-reactive antibody responses, overall T cell immunity, breadth of T cell epitopes, and protection against influenza virus. The primary goal of this study is to clone the influenza …

Spider Prey Of Mud-Dauber Wasps In Southeastern Nebraska, Earl Agpawa, Tyler Corey, Eileen Hebets

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Predator-prey interactions between organisms provide a window into behaviors, adaptions, and evolutionary histories of both groups. One group of organisms of would be female spider-specialist mud-dauber wasps (Hymenoptera: Sphecidae) who seek out and sting spiders to bring back to their nests for their larvae to feed upon. This interaction provides a unique means of quantifying the diversity and abundance of the prey they capture. In this study, we inventoried spiders from within collected nests of mud-dauber wasps Sceliphron caementarium (Drury) and Chalybion californicum (Saussure) from agricultural land and in a forest corridor. It was found that nests collected in agricultural …

Sorghum Tolerance Of Phloem-Feeding Aphids, Earl Agpawa, Sajjan Grover, Joe Louis

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Aphids are phloem sap-feeding insects that negatively affects plant productivity. With short generation times, new aphid biotypes may arise either in response to pesticide use or through other factors. Additional biotypes can cause serious damage to plants compared to others. Whereas others can become highly resistant to methods of pest control. One possible solution to this problem is to focus on the plant itself, or host plant resistance. Plant tolerance is one of the plant resistance categories in which the plant is capable of functioning normally and remain relatively unharmed and stable in response to insect herbivory without harming the …

Racial-Ethnic Disparities In Prediabetes And Diabetes Among Us Adults, Carly Selleck, Jennifer R. Mcatee, Weiwen Chai

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Type-2 diabetes is a chronic metabolic disease affecting more than3 4 million Americans or 10.5% of the population [1]. While almost 90% of people are not aware of their prediabetes status, around 88 million adults are considered to be prediabetic [2]. Without any prevention methods, 70% of prediabetes cases will eventually develop into diabetes[3]. While the development of type-2 diabetes is multifactorial, lifestyle factors such as diet and physical activity as well as socioeconomic (SES) factors such as poverty and education can be main contributors [4]. This study aimed to assess racial-ethnic differences in SES, lifestyle factors and prevalence of …