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University of Nebraska - Lincoln

Department of Entomology: Faculty Publications

2015

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Full-Text Articles in Life Sciences

Transcriptional Profiling Of Resistant And Susceptible Buffalograsses In Response To Blissus Occiduus (Hemiptera: Blissidae) Feeding, Crystal M Ramm, Michael Wachholtz, Keenan Amunsen, Teresa J. Donze, Tiffany Heng-Moss, Paul Twigg, Nathan A. Palmer, Gautam Sarath, Frederick P. Baxendale Jan 2015

Transcriptional Profiling Of Resistant And Susceptible Buffalograsses In Response To Blissus Occiduus (Hemiptera: Blissidae) Feeding, Crystal M Ramm, Michael Wachholtz, Keenan Amunsen, Teresa J. Donze, Tiffany Heng-Moss, Paul Twigg, Nathan A. Palmer, Gautam Sarath, Frederick P. Baxendale

Department of Entomology: Faculty Publications

ABSTRACT Understanding plant resistance mechanisms at a molecular level would provide valuable insights into the biological pathways impacted by insect feeding, and help explain specific plant tolerance mechanisms. As a first step in this process, we conducted next-generation sequencing using RNA extracted from chinch bug-tolerant and -susceptible buffalograss genotypes at 7 and 14 d after chinch bug feeding. Sequence descriptions and gene ontology terms were assigned to 1,701 differentially expressed genes. Defense-related transcripts were differentially expressed within the chinch bug-tolerant buffalograss, Prestige, and susceptible buffalograss, 378. Interestingly, four peroxidase transcripts had higher basal expression in tolerant control plants compared with …