Life Sciences Commons^™

Retinoic Acid Hydroxylase Inhibitors As A Novel Therapy For Alzheimer’S Disease, Isabel M. Makman

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Alzheimer’s Disease (AD) is one of the most prevalent neurodegenerative diseases afflicting the modern world. As no cure for AD has yet been discovered we sought to explore a potential treatment option based on the inhibition of retinoic acid (RA) metabolism, the active metabolite of vitamin A. Beta-amyloid plaques form in the brain and decrease cognitive function in AD patients. In a recent preclinical study RA was shown to decrease these plaques and rescue memory deficits in an Alzheimer’s mouse model[i]. One key limitation is that when RA is administered to humans it induces its own breakdown, making …

Wolf-Cougar Co-Occurrence In The Central Canadian Rocky Mountains, Ellen Brandell

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Cougars and wolves are top carnivores that influence the dynamics of an ecosystem, including prey behavior and dynamics, and interspecific competition. Studies about the interactions between wolves and cougars typically find wolves are dominant competitors to cougars. We examined single-species, single-season occupancy models and co-occurrence models of wolves and cougars in the Central Canadian Rocky Mountains to understand interactions between these two species on a grand landscape. Data was collected from 2012-2013 using remote wildlife cameras and separated into seasons. Naïve occupancy estimates were larger for wolves in both seasons, but both species had smaller ranges in winter. There were …

Why Do Caterpillars Whistle? Acoustic Mimicry Of Bird Alarm Calls In The Amorpha Juglandis Caterpillar, Jessica Lindsay

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Caterpillar species possess a range of anti-predator defenses, from regurgitation to sonar jamming. The North American walnut sphinx caterpillar (Amorpha juglandis) produces a variety of whistling noises when pinched. Limited observations indicate that this causes avian predators to retreat, leaving the caterpillar alone. However, it is unknown why this whistle is such an effective deterrent. Interestingly, the A. juglandis whistle is acoustically similar to the “seet” alarm call that many bird species produce in response to their own predators. We propose that the A. juglandis whistle is a form of acoustic mimicry, in which the caterpillar protects itself …

Decadal Scale Responses Of Soil And Ecosystem Processes To Forest Restoration In Rocky Mountain Conifer Forests, Peter Ganzlin

Graduate Student Theses, Dissertations, & Professional Papers

Decades of fire suppression have left much of the forest in the intermountain western United States exceedingly dense, and forest restoration techniques – including thinning and prescribed fire – are increasingly being used in an attempt to mitigate the potentially disastrous effects of severe wildfire, to enhance tree growth and regeneration, and to stimulate soil nutrient cycling. While many of the short-term effects of forest restoration have been established, the long-term effects on soil biogeochemical and ecosystem processes are largely unknown. In this thesis I present two manuscripts documenting and synthesizing these long-term impacts. The first chapter focuses on the …

The Use Of Rna Interference To Mitigate Pulmonary Fibrosis In Response To Asbestos Exposure, Sarah Kinsey

Undergraduate Theses, Professional Papers, and Capstone Artifacts

The adverse health effects of exposure to asbestos are widely known and have been well documented. When a person is diagnosed with asbestosis, a chronic lung disease caused by inhaling asbestos fibers, few treatment options exist, none of which halt or reverse the progression of the disease. The rapidly growing field of gene therapy offers new avenues for potential treatments worthy of investigation. The detrimental effects of asbestos exposure are due to the physiological response of the lungs to asbestos fibers in the form of fibrosis, a result of excess extracellular collagen deposition. A protein called SPARC (Secreted Protein Acidic …

Off The Court, Onto The Farm, Arza E. Hammond

Graduate Student Theses, Dissertations, & Professional Papers

This work contains two separate but connected stories about developing a relationship with something larger than myself. Reaching the end of my basketball career forced me to examine my relationship with the great game of basketball and my role in it. After ending my hoops career, I spent a summer working and living on a small, organic farm. The lessons I learned from the game helped me to develop a meaningful relationship with the farm and to find a reciprocal role with the land I lived and worked on.

A Critical Examination Of Private Conservation Areas On Campesino Community Lands In Peru, Megan Barnhart

Graduate Student Theses, Dissertations, & Professional Papers

Community-owned private reserves are emerging around the world as an alternative to government-run resource management and as a way to more directly involve citizens as stewards of their local natural resources. Despite their recent proliferation globally, voluntary efforts by communities to include their land in protected area systems, and the motivations and expectations of their legal recognition remain largely unknown. This thesis examines community-owned private conservation areas in Northern Peru locally known as Áreas de Conservación Privadas (ACPs) which are voluntary and legally recognized by the Peruvian State. The study investigates the rationales and outcomes of the application of ACPs …

An Assessment Of Biochar Amended Soilless Media For Nursery Propagation Of Northern Rocky Mountain Native Plants, Clarice P. Matt

Graduate Student Theses, Dissertations, & Professional Papers

A study was conducted to better inform nursery practitioners of the potential benefits of biochar application in common nursery media for native plant propagation. Biochar – a carbon-rich, recalcitrant charred organic co-product of the bioenergy pyrolysis process – has emerged as a promising potential replacement for various components of soilless media, namely peat, perlite, and vermiculite, in nursery seedling propagation. A strong conceptual basis exists for biochar as a nursery media amendment, but empirical data on biochar-based plant propagation is limited. This greenhouse study examined the effects of biochar displacement of standard soilless nursery media at rates of 0%, 15%, …

Abundance, Density, And Opinions About Columbian Black-Tailed Deer, Whidbey Island, Washington, Robert P. Wingard

Graduate Student Theses, Dissertations, & Professional Papers

Obtaining reliable knowledge is the first step towards properly managing wildlife species. Columbian black-tailed deer (Odocoileus hemionus columbianus) on Whidbey Island, Washington have not been the subject of study, and little is known about the population, or the opinion of resident’s of Whidbey Island towards the deer population. However, wildlife managers suggest deer on the island may be overabundant or over social carrying capacity. Given the lack of empirical knowledge about the deer population or human opinions towards deer on Whidbey Island, I designed research to determine the abundance and density of Columbian black-tailed deer on Whidbey Island, …

Cows And Plows: Science-Based Conservation For Grassland Songbirds In Agricultural Landscapes, Marisa K. Lipsey

Graduate Student Theses, Dissertations, & Professional Papers

Temperate grasslands are among earth’s most imperiled ecosystems. In North America, steep declines of endemic songbird populations indicate that grassland loss and degradation may be approaching critical levels. Grasslands are agricultural landscapes largely (~85%) under private ownership with little formal protection status. Remaining bird populations depend on grazing lands that have not been converted to cropland. We combine regional data from a hotspot for grassland bird diversity (northeast Montana, USA; 26,500-km2) with continental data spanning the northern Great Plains (1,000,000-km2) to evaluate how land use and management influence bird distribution and abundance. Regionally, habitat used by seven grassland specialists spanned …

A Model For Determining Drivers Of Phenology In Western United States Rangelands, Joseph R. St. Peter

Graduate Student Theses, Dissertations, & Professional Papers

Plant phenology has long been used as an indicator of climate. Recent changes in plant phenology are evidence of the influence of climate change. Modeling plant phenology has become an effective tool to understand the impacts of climate change. Using machine learning techniques I developed a modeling process for accurately predicting phenology across a diverse landscape. This model uses individual site data to set site specific climate thresholds for plant phenology. This model also identifies the limiting factors to vegetation phenology for rangelands in the western United States. NDVI remotely sensed data was used to quantify land surface phenology and …

Impact Of A Flame Resistant Synthetic Material Base Layer On Heat Stress Factors, Matthew C. Dorton

Graduate Student Theses, Dissertations, & Professional Papers

Protective clothing worn by wildland firefighters (WLFF) may increase physiological strain and heat stress factors due to increased insulation and decreased ventilation. PURPOSE: To examine the effects of a flame resistant synthetic material base layer on heat stress factors. METHODS: Ten recreationally active males (25 ± 6.1 yrs, 80.9 ± 8.4 kg, 11.1 ± 5.3% fat, 4.4 ± 0.6 L·min-1 VO2 max) completed two trials of intermittent (50 min walking, 10 min sitting) treadmill walking (4km/hr, 4% grade) over 3 hours in a hot, dry environment (35⁰C, 30% rh). Participants wore standard WLFF Nomex green pants, Nomex yellow shirt with …

Assessing Changes In Avian Communities, Jessie D. Golding

Graduate Student Theses, Dissertations, & Professional Papers

Grazing is a potentially powerful tool to address wildlife declines associated with land use conversion in the western United States. Grazing systems can be manipulated to achieve desired vegetation outcomes, preserve native habitat and economically benefit multiple stakeholders. As a result, systems designed to benefit native ecosystems are being widely implemented. However, the benefits of these grazing systems on many wildlife communities remain relatively unexplored. Songbirds provide an ideal study system to test these benefits because they continue to use native habitat that is currently grazed. Given limited time and resources, conservation practitioners often monitor a single focal species or …

A Case Study Tested Framework For Multivariate Analyses Of Microbiomes: Software For Microbial Community Comparisons, Eric M. Spaulding

Graduate Student Theses, Dissertations, & Professional Papers

The study of microbiomes is important because our understanding of microbial communities is providing insight into human health and many other areas of interest. Researchers often use genomic data to study microbial organisms, demonstrating differences from one organism to the next. Metagenomic data is utilized to study communities of microbial organisms. The research described herein involved the development of a collection of computational methods.

This suite of computational methods and tools (written in the R and Perl languages) has become a framework used for metagenomic data analysis and result visualization. Multivariate analyses such as Linear Discriminate Analysis (LDA) are used …

Thermodynamics Of Hhr23a Ubiquitin-Associated (Uba) Domains, Moses Joseph Leavens

Graduate Student Theses, Dissertations, & Professional Papers

Ubiquitin-associated (UBA) domains have been discovered in several proteins within the cell, and at least one domain plays an important functional role in cellular phenomena such as NF-κB signaling. Mutations within the UBA(2) domain of the ubiquitin-binding scaffold protein p62 are frequently observed in patients suffering from Paget’s disease of bone. The human DNA excision repair protein HHR23A contains the UBA(1) and UBA(2) domains, and nuclear magnetic resonance (NMR) structures depict their compact three-helix bundles. Moreover, the HHR23A UBA(1) and UBA(2) only share approximately 20% primary sequence identity. The p62 UBA(2) domain has been shown to contain quaternary structure in …

Population Differentiation And Habitat Selection Of A Montane Red Fox Population In The Greater Yellowstone Ecosystem, Patrick Cross

Graduate Student Theses, Dissertations, & Professional Papers

Montane red fox (Vulpes vulpes) populations across the western United States are genetically and morphologically distinct from foxes at lower elevations. These montane populations also share a preference for subalpine forest habitats. One hypothesis is that they stem from boreal forest-associated ancestors that expanded during the Pleistocene when boreal forests extended farther south than they do today. Forest habitat selection may therefore aid the persistence of native populations surrounded by non-native conspecifics. Alternatively, this behavior may be an avoidance mechanism in response to competition with larger coyotes (Canis latrans), or a product of the fox's natural adaptability. The red fox …

Developing Microbial Biomarkers To Non-Invasively Assess Health In Wild Elk (Cervus Canadensis) Populations, Samuel B. Pannoni

Undergraduate Theses, Professional Papers, and Capstone Artifacts

The composition of the intestinal bacterial community (intestinal microbiome) of mammals is associated with changes in diet, stress, disease and physical condition of the animal. The relationship between health and the microbiome has been extensively demonstrated in studies of humans and mice; this provides strong support for its potential utility in wildlife. When managing elk (Cervus canadensis), federal and state agencies currently must rely on invasive sampling and coarse demographic data on which to base their decisions. By developing microbiome-based biomarkers that vary as a function of elk body condition and disease (i.e. microbial biomarkers), we hope to …

The Sensitivity Of Soil Respiration To Soil Temperature, Moisture, And Carbon Supply At The Global Scale, Andrew Hursh

Graduate Student Theses, Dissertations, & Professional Papers

Soil respiration is one of the most important terms in the global carbon budget, yet we know very little about how important environmental factors control this process at the global scale. Soils contain more carbon than terrestrial biomass and the atmosphere combined and contribute ten times more carbon dioxide to the atmosphere each year than the burning of fossil fuels. This study provides new insight on the factors driving soil respiration at the global scale by assimilating satellite observations of soil moisture, temperature, and net primary productivity with the Global Soil Respiration Database (SRDB).While temperature, moisture, carbon supply and other …

Chromosomal Rearrangements Directly Cause Underdominant F1 Pollen Sterility In Mimulus Lewisii-M. Cardinalis Hybrids, Angela M. Stathos

Graduate Student Theses, Dissertations, & Professional Papers

Chromosomal rearrangements can contribute to the evolution of postzygotic reproductive isolation directly, by disrupting meiosis in F₁ hybrids, or indirectly, by suppressing recombination among genic incompatibilities. Because direct effects of rearrangements on fertility imply fitness costs during their spread, understanding the mechanism of F₁ hybrid sterility is integral to reconstructing the role(s) of rearrangements in speciation. In hybrids between monkeyflowers Mimulus cardinalis and M. lewisii, rearrangements contain all quantitative trait loci (QTLs) for both premating barriers and pollen sterility, suggesting that they may have facilitated speciation in this model system. I used artificial chromosome doubling and …

Community Perturbation Associated With New Zealand Mudsnail (Potamapyrgus Antipodarum) Invasion: Discrepancies In Temporal And Spatial Assessment, Eric H. Richins

Graduate Student Theses, Dissertations, & Professional Papers

Invasive species have the potential to alter the ecosystems within which they establish as well as adjacent but uninvaded ecosystems by altering the flow of nutrients or biota across system boundaries. New Zealand mudsnails (Potamopyrgus antipodarum; NZMS) are often very abundant where they invade, sequestering the majority of stream primary production. This sequestering of production may reduce aquatic insect production, subsequent insect emergence and subsidies to riparian ecosystems. Tetragnathid spiders, an insectivorous riparian specialist, are dependent on the emergence of aquatic insects and are typical indicators of aquatic insect subsidies to riparian zones. In addition, NZMS invasions can result …

Gait Intervention For Improvements In Human Top Speed Running, Michelle Buechner

Graduate Student Theses, Dissertations, & Professional Papers

Between individuals faster sprinting speeds are achieved by applying greater stance average forces against the running surface. Recent evidence further indicates that elite level performers also strike the ground with leg kinematics that differ from those of non-elites and that these leg movements act to enhance the force transients occurring in the milliseconds following foot-ground impact. I investigated whether sprint performance could be enhanced through a short term gait intervention, consisting of 3 laboratory training sessions wherein subjects (n = 6) completed 5 high-speed runs on an instrumented force treadmill at 90% of their measured top sprint. The subjects received …

The Role Of Dksa In The Stringent Response In The Lyme Disease Spirochete Borrelia Burgdorferi, Britney Cheff

Graduate Student Theses, Dissertations, & Professional Papers

Borrelia burgdorferi, the bacterium that causes Lyme disease, is maintained in nature through an enzootic cycle that includes a tick vector and a vertebrate host. The bacterium is acquired by an Ixodes tick from an infected vertebrate. The bacterium alters its gene expression to adapt to different environments of the tick and vertebrate. Between tick feedings, B. burgdorferi must contend with nutrient stress. The stringent response is a physiological mechanism when bacteria switch from “thriving” to “surviving” mode in response to limited nutrient resources; it is mediated by an increase in the nucleotide alarmone guanosine penta- or tetraphosphate, abbreviated …

Bamboo Harvesting For Household Income Generation In The Ethiopian Highlands: Current Conditions And Management Challenges, Bridget L. Tinsley

Graduate Student Theses, Dissertations, & Professional Papers

Bamboo is a renewable resource that has been advocated as a means to alleviate poverty and foster rural development throughout the world. Ethiopia holds 67% of continental Africa’s bamboo coverage and is gaining interest by international markets. Despite great speculation about Ethiopia’s bamboo market potential, foundational information regarding household utilization and income reliance is lacking. To understand how bamboo contributes to rural Ethiopian households, a quantitative household assessment was undertaken in this study. A questionnaire census collected data from 371 households. A quantitative assessment of household incomes and assets evaluated what factors influence bamboo harvesting.

The contribution of bamboo to …

Use Of Airborne Digital Imagery To Examine Floodplain Complexity At Varying Discharges, Katelyn P. Driscoll

Graduate Student Theses, Dissertations, & Professional Papers

Floodplains are composed of aquatic and terrestrial habitats that are frequently reshaped by hydrologic processes operating at various spatial and temporal scales. I hypothesized that floodplain habitat complexity is maximized at intermediate discharges because small changes in flow result in substantial aquatic habitat changes and extreme discharges are associated with a decreased habitat heterogeneity. Between April and September 2014, I collected ultra-high resolution digital multispectral imagery of the Clark Fork River, Montana taken on 6 dates between early spring and fall. Following image mosaicking into a single image, unsupervised classification of the spectral reflectance was used to identify and quantify …

Ecological Status Of Black-Tailed Prairie Dogs On Boulder, Colorado Open Space And Mountain Parks Land: An Analysis Of Select Indicators, Rachel A. Caldwell

Graduate Student Theses, Dissertations, & Professional Papers

Black-tailed prairie dogs (Cynomys ludovicianus) are ecosystem regulators, significantly influencing the surrounding ecosystem. On Boulder County, Colorado Open Space and Mountain Parks land, and throughout their range, prairie dogs affect a number of ecological functions including plant dynamics and associated animals. The ability of prairie dogs to promote the wellbeing of associated plant and animal species depends on the condition of prairie dog populations. One way to evaluate the current condition of prairie dogs and predict their future impact on the ecosystem is to evaluate the status of prairie dogs in relation to specific indicators. I address the …

Forest Fire Risk Assessment Using Point Process Modeling & Monte Carlo Fire Simulation: A Case Study In Gyeongju, South Korea, Hyeyoung Woo

Graduate Student Theses, Dissertations, & Professional Papers

Forest fire risk assessment becomes critical for developing forest and fire management strategies in Korea since the magnitude of damage from fires significantly increased over the past decades. Fire behavior probability is one of the major components in quantifying fire risk, and is often presented as burn probability. Burn probability estimation requires a proper estimation of fire occurrence probability because fire spread is largely influenced by ignition locations in addition to other environmental factors, such as weather, topography, and land covers.

The objective of this study is to assess forest fire risk over a large forested landscape in and around …

A Gis Assessment Of Ecoregion Representation In Chile's Existing And Proposed Integrated Network Of Protected Areas, Jessica Schutz

Graduate Student Theses, Dissertations, & Professional Papers

Chile's state designated protected areas are reported to show representation bias and to be unable to meet conservation goals. Private protected areas are considered an important tool to resolve these issues, which has led to support for increasing the role of private protected areas in Chile and creating an integrated public-private protected area network. But the validity of the capacity of private protected areas to fix Chile's state protected area network bias, and the advantage of creating an integrated protected area network, have not been assessed. This study uses the most recent data on Chile's state, private, and international protected …