Life Sciences Commons^™

Purification And Characterization Of Novel Nucleases From A Thermophilic Fungus, Kyle S. Landry

Masters Theses 1911 - February 2014

A thermophilic fungus was isolated from composted horse manure. The organism was as a Chaetomium sp. by sequencing the highly conserved ITS region of the fungus and comparing to known regions in a genomic database and was referred to as TM-417. TM-417 was found to have an optimal growth temperature of 45 ^oC and an optimal pH of 7.0. An extracellular DNase and RNase was found to be produced by the isolate and were purified 145.58-fold and 127.6-fold respectively using a combination of size exclusion chromatography and a novel affinity membrane purification system. The extent of purification was determined …

Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells, Michael E. Urbanowski

Masters Theses 1911 - February 2014

The regulation of pH is a critical homeostatic function of plant cells. In addition to acting as the primary cationic species responsible for energizing the plasma membrane, protons likely act as an important regulator and messenger. Despite this importance, few studies have thoroughly described cytosolic pH patterns as the plant cell progresses through the cell cycle. To investigate pH in plant cells, I chose Nicotiana tabacum (tobacco) Bright Yellow-2 (BY-2) cells as a model system. My research has two aims. First, I will measure and report the interphase cytosolic pH of BY-2 cells. Next, I will assay the cytosolic pH …

Use Of Short-Term Floods As An Additional Management Strategy For Controlling Dodder (Cuscuta Gronovii Willd.) In Commercial Cranberry Production, James M. O'Connell

Masters Theses 1911 - February 2014

Dodder (Cuscuta gronovii Willd.) is a weed of serious concern to cranberry (Vaccinium macrocarpon Ait.) growers. It develops vigorously and has a long-lived seed bank. Cranberries are a perennial crop and therefore strategies available to growers of annual crops are not practical. Herbicides, the primary management tool for dodder, although effective, have a narrow window of application and extended seedling emergence after applications can result in escapes. This project examined the effect of water temperature on dodder seed germination and the use of short-term floods (less than 72 hr) for dodder management.

Experiments investigated the effect of water temperature on …

Spring 1985, Chris Beasley, James Beard, Robert N. Carrow, Chuck Wilson

Turf Bulletin

1. The New England Sod Industry (page 3)
2. New Trends and Research in Turfgrass Culture (5)
3. Fifty-Fourth Annual Turf Conference and Ninth Industrial Show
4. Irrigation Programming (13)
5. Landscape Development in Large Residential Communities (17)

Spring 1984 Conference Issue, William A. Torello, Anna G. Symington, Charles F. Mancino, Elizabeth L. Clifton, Lesley A. Spokas, Joseph Troll, John M. Zak, Peter H. Dernoeden

Turf Bulletin

1. Research Update (page 3)
2. Low Management Kentucky Bluegrass Cultivar Trials (5)
3. Nitrogen Leaching Losses from N Fertilizer Applied to Turf (8)
4. Fifty-third Annual Turf Conference and Eighth Industrial Show (10)
5. Liquid Versus Dry Nitrogren Fertilizer Applications on Kentucky Bluegrass (13)
6. Growth Retardant, Embark (16)
7. Fungicides: The Good, The Bad and The Ugly (18)

Spring 1983 Conference Issue, Anna G. Symington, Mickey Spokas, Joseph Troll, Nancy L. Garrabrants, David K. Bell

Turf Bulletin

1. Chemical Retardation of Grass Growth (page 3)
2. Cultivar Trials - 1982 (5)
3. Fifty-second Annual Turf Conference and Seventh Industrial Show (10)
4. Dollar Spot Fungicide Control Trials - 1982 (13)
5. Winter Protection of Herbaceous Perennials (14)
6. Control of Annual Bluegrass in Golf Tees Using Perennial Ryegrass and Ethofumesate (15)

Fall 1983, Pat Lucas, Ann Reilly, J. D. Butler, C. M. Feldhake, R. H. Hurley, C. Reed Funk, Prasanta C. Bhowmik, Roy L. Goss

Turf Bulletin

1. Canada Geese Control (page 3)
2. Coloring the Course (5)
3. Irrigation's Role for Turf and Landscape (7)
4. Perennial Ryegrass Update (12)
5. Crabgrass - A Common Troublesome Weed in Turfgrass (15)
6. Thatch and Its Control (18)

Spring 1982 Conference Issue, Nancy N. Ragsdale, William A. Torello, William B. Davis, Jack A. Paul

Turf Bulletin

1. Turf Clippings (page 3)
2. How the RPAR Process is Working (4)
3. Cellular and Genetic Engineering Techniques Applied Towards the Improvement of Turfgrass (7)
4. Fifty-First Annual Turf Conference and Sixth Industrial Show (10)
5. Natural Versus Artificial Turf--an Economical Alternative (13)
6. Fertility Assay of Sands (17)

Fall 1982, Roland D. Hauck, Anne Moffat, Roger Funk

Turf Bulletin

1. Turf Clippings (page 3)
2. Using Nitrogen Efficiently - Theoretical Aspects (4)
3. Life After Frost (12)
4. Liability and The Lawn Care Industry (16)

Summer 1982, Patricia J. Vittum, J. M. Vargas Jr., Joseph Troll, Mickey Spokas, David F. Karnosky, Clive G. Jones

Turf Bulletin

1. Registration of Isofenphos (page 3)
2. Managing Annual Bluegrass (4)
3. Helminthosporium Leaf Spot Fungicide Control Trial--1981 (8)
4. Cultivar Trials--1981 (9)
5. Dollar Spot (Sclerotinia Homeocarpa Sp.) Fungicide Control Trial--1981 (14)
6. Living with the Gypsy Moth (15)

Spring 1981 Conference Issue, Ruth S. Zak, Daniel Hillel, Joseph Troll

Turf Bulletin

1. Fifty Years of Turf Management Education at the University of Massachusetts (page 3)
2. Commentary: A Case for Conditional Optimism (7)
3. Fiftieth Annual Turf Conference and Fifth Industrial Show (10)
4. Dollar Spot Fungicide Trials --1980 (12)
5. Tree News (14)
6. Insurance Requirements (16)
7. UMass Turfgrass Research Fund (18)
8. Exams (19)

Summer 1981, Ellery L. Knake, T. L. Larry, J. F. Wilkinson, R. H. Miller, Carol A. Lembi, Ted Horton, Horace Ames

Turf Bulletin

1. Editorial (page 3)
2. Results of Field Test to Study Human Exposure to 2,4,5,-T Application (4)
3. Investigation and Treatment of Localized Dry Spots on Sand Golf Greens (6)
4. Aquatic Weed Control- In Review (11)
5. Golf Course Superintendent - A Perspective (14)
6. Four Seasons Ground Maintenance (16)

Fall 1981, Patricia J. Vittum, Dan Paulson Jr., Bob Early, Elmer R. Fleck

Turf Bulletin

1. Turf Clippings (page 3)
2. Registration of Oftanol^R (Isofenphos) (4)
3. Effect of pH Organic Matter and Solid Texture on Herbicides (6)
4. Lawn Care: Big Potential for Fluids

Summer 1980, K. A. Hurto, Ruth Foster

Turf Bulletin

1. Principles of Turfgrass Weed Control III. Broadleaf Weeds (page 3)
2. To Stop Leaks, First Find Them (6)
3. Turgrass Research Field Day (9)
4. Gypsy Moth (10)
5. Try Ground Covers for Low-Maintenance (13)
6. Pesticide News (18)

Fall 1980, Alan Bebka, Alfred W. Boicourt, Robert N. Carrow, James O'Kelly

Turf Bulletin

1. Chronic Organo-Phosphate Poisoning (page 3)
2. Perennials (7)
3. Topdressing (10)
4. Winter Problems of Shade Trees (15)
5. Fall Color (18)

Spring 1980 Conference Issue, John O'Connor, William A. Meyer

Turf Bulletin

1. Plastic Water Pipe: How Well Does it Hold Up? (page 3)
2. Forty-Ninth Annual Turf Conference and Fourth Industrial Show Program (10)
3. Blend Bluegrasses for Best Disease Resistance (13)
4. Dollar Spot Fungicide Trials--1979 (16)
5. UMass Turfgrass Research Fund (18)

Spring 1979 Conference Issue, K. A. Hurto, Alfred W. Boicourt, W. A. Sinclair, E. S. Pira

Turf Bulletin

1. Principles of Turfgrass Weed Control Annual Grasses (page 3)
2. Bean-Shape Islands (7)
3. High Protein Food From Grass (8)
4. Forty-Eighth Annual Turf Conference and Third Industrial Show Program (10)
5. "Dutch Elm Disease: Perspectives After 60 Years" (13)
6. Toro Irrigation Design Seminar (14)
7. Ideas: New and Old (15)
8. More Pesticide Exams (16)
9. Disposal of Pesticides in Massachusetts (18)
10. Moth Controls Nutsedge Weeds (19)
11. New Pesticide Bill (20)

Fall 1979, Walter C. Shortle, Jeff Cox, Francis W. Holmes, Clifford S. Chater, Anthony Decrosta

Turf Bulletin

1. New Ideas in Tree Care (page 3)
2. 'Wonder Water' (6)
3. Herbicides: Are They Safe? (9)
4. University of Massachusetts Turfgrass Field Day (10)
5. The Shade Tree Laboratories of Massachusetts--Origin and Public Services (11)
6. Do Pesticides Cause Allergies (14)
7. Federal Noxious Weed Act Funded (18)
8. Your Opinion Please (18)

Summer 1979, Joseph Troll, K. A. Hurto

Turf Bulletin

1. Fertilizer Programs for Bluegrass and Ryegrasses (page 3)
2. Turfgrass Slide Sets Available (6)
3. Seed Extracts Repel Japanese Beetles (8)
4. Principles of Turfgrass Weed Control (10)
5. How Regulation is Impacting on Pesticide Research (12)
6. UMass Turfgrass Research Fund (20)
7. UMass Turfgrass Research Field Day (20)

Winter 1979, John Tristan, Joe Don Boyd, Fred P. Miller, K. A. Hurto, Pat Kristy, Joseph Troll

Turf Bulletin

1. Clubhouse Plants (page 3)
2. Massachusetts Pesticide News (6)
3. The Alsea Report on 2,4,5-T (8)
4. Better Pest Control (10)
5. Acid Rain: Something Else to Worry About? (11)
6. UMass Turfgrass Research Update (13)
7. 1979 Preemergence Crabgrass Control Trial

Spring 1987 Conference Issue, Brian M. Silva, J. Troll, M. Bryant, Richard Dattner

Turf Bulletin

1. Phosphorus and Its Effect on Plant Growth (page 3)
2. Dollar Spot Fungicide Control Trial--1977 (9)
3. Forty-Seventh Annual Turf Conference and Second Industrial Show Program (10)
4. Parks for the Year 2000 (15)

Fall 1978, Harry C. Eckhoff, Kent W. Kurtz, Carol Dana

Turf Bulletin

1. Guidelines for Planning and Building a Golf Course (page 3)
2. Turfgrass Field Day (6)
3. Problems in Managing Turfgrass in Shade (9)
4. Escape to the Islands--in Boston Harbor (15)

Summer 1978, R. H. Miller, J. F. Wilkinson, Joseph Troll, Kirk Hurto, Joseph Levy

Turf Bulletin

1. Nature of the Organic Coating on Sand Grains of Nonwettable Golf Greens (page 3)
2. Massachusetts Turfgrass Winter Injury (6)
3. Investigation and Treatment of Localized Dry Spots on Sand Golf Greens (8)
4. A Recreation Renaissance (15)
5. UMass Turfgrass Research Fund (20)

Winter 1978, William Emerson, O. A. Christiansen, John M. Zak

Turf Bulletin

1. The Psychological Approach to Golf Course Management (page 3)
2. Massachusetts Pesticide News (6)
3. Cross-Country Skiing: Good Winter Recreation that Pays its Own Way (8)
4. Sacrification of Large Scale Areas, e.g. Golf Course Fairways, Winter Games Areas, Etc. (15)
5. Plant Materials for Outlying Areas
6. Biological Control of Crop Diseases (18)

Summer 1977, Daniel I. Levy, John R. Hall Iii, Tom Gentle, Carl F. Gortzig

Turf Bulletin

1. Solar Energy: Wave of the Future? (page 3)
2. Turfgrass Nutrition: Current Thinking and Future Challenges (7)
3. Make Wine--Not Waste--with Whey (12)
4. Potted Florist Crops for Everyday Use (15)
5. UMass Turfgrass Research Fund (19)

Winter 1977, K. W. Brown, R. L. Dubie, Brian M. Silva, J. F. Wilkinson

Turf Bulletin

1. Influence of Management and Season on Fate of N Applied to Golf Greens (page 3)
2. Potassium: The Mystery Element (9)
3. Effect of IBDU and UF Rate, Date and Frequency of Application on Merion Kentucky Bluegrass (14)

Spring 1977, Neal Weaver, Joseph Troll, Robert N. Carrow, John Griffin

Turf Bulletin

1. Thomas Jefferson (page 3)
2. Herbicide Evaluation for Crabgrass Control--1976 (9)
3. Fourty-Sixth Annual Turf Conference and First Industrial Show Program (10)
4. Dollar Spot Fungicide Control Trial--1976 (13)
5. All About Gardening (14)
6. Herbicide Trials on Broadleaf Weeds and a Phytotoxicity Test--1976 (17)
7. Helminthosporium Leaf Spot Fungicide Control Trial--1976 (17)
8. Microbes--Cleaner-uppers for Polluted Waters (18)

Winter 1976, Robert W. Shery, R. N. Carrow, J. Troll, Jeffery Nedelman

Turf Bulletin

1. Lawn Fertilization (3)
2. Turf Conference Reminder (4)
3. Perennial Ryegrass Variety Trial--Performance in 1976 (5)
4. Red Fescue Variety Tiral--Performance in 1976 (7)
5. University of Massachusetts Turfgrass Research Fund (10)
6. Will Congress Control the Chemical Industry? (11)
7. Kentucky Bluegrass Variety Trial 1973--Performance in 1976 (14)
8. Kentucky Bluegrass Variety Trial 1974--Performance in 1976 (18)

Winter 1976, Robert W. Shery, R. N. Carrow, J. Troll, Jeffrey Nedelman

Turf Bulletin

1. Lawn Fertilization (page 3)
2. Turf Conference Reminder (4)
3. Perennial Ryegrass Variety Trial--Performance in 1976 (5)
4. Red Fescue Variety Trial--Performance in 1976 (7)
5. University of Massachusetts Turfgrass Research Fund (10)
6. Will Congress Control the Chemical Industry (11)
7. Kentucky Bluegrass Variety Trial 1973--Performance in 1976 (14)
8. Kentucky Bluegrass Variety Trial 1974--Performance in 1976 (18)

Fall 1976, William Rice, Rex Gogerty, Victor Gibeault, Sherwood A. Moore, Joseph Troll

Turf Bulletin

1. Lawn & Turf Seed Labeling--Are Changes Needed? (page 3)
2. Weed Control: More Options Ahead (6)
3. Problems and Solutions Associated with Heavily Used Turfgrass Areas (12)
4. Past and Present Golf Course Maintenance (15)
5. Tractor Safety Begins with ROPS (19)