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Full-Text Articles in Life Sciences
Two Decades Of Warming Increases Diversity Of A Potentially Lignolytic Bacterial Community, Grace Pold, Jerry M. Melillo, Kristen Deangelis
Two Decades Of Warming Increases Diversity Of A Potentially Lignolytic Bacterial Community, Grace Pold, Jerry M. Melillo, Kristen Deangelis
Microbiology Department Faculty Publication Series
As Earth's climate warms, the massive stores of carbon found in soil are predicted to become depleted, and leave behind a smaller carbon pool that is less accessible to microbes. At a long-term forest soil-warming experiment in central Massachusetts, soil respiration and bacterial diversity have increased, while fungal biomass and microbially-accessible soil carbon have decreased. Here, we evaluate how warming has affected the microbial community's capability to degrade chemically-complex soil carbon using lignin-amended BioSep beads. We profiled the bacterial and fungal communities using PCR-based methods and completed extracellular enzyme assays as a proxy for potential community function. We found that …
Genome Sequence And Description Of The Anaerobic Lignin-Degrading Bacterium Tolumonas Lignolytica Sp. Nov., Andrew F. Billings, Julian L. Fortney, Terry C. Hazen, Blake Simmons, Karen W. Davenport, Lynne Goodwin, Natalia Ivanova, Nikos C. Kyrpides, Konstantinos Mavromatis, Tanya Woyke, Kristen Deangelis
Genome Sequence And Description Of The Anaerobic Lignin-Degrading Bacterium Tolumonas Lignolytica Sp. Nov., Andrew F. Billings, Julian L. Fortney, Terry C. Hazen, Blake Simmons, Karen W. Davenport, Lynne Goodwin, Natalia Ivanova, Nikos C. Kyrpides, Konstantinos Mavromatis, Tanya Woyke, Kristen Deangelis
Microbiology Department Faculty Publication Series
Tolumonas lignolytica BRL6-1T sp. nov. is the type strain of T. lignolytica sp. nov., a proposed novel species of the Tolumonas genus. This strain was isolated from tropical rainforest soils based on its ability to utilize lignin as a sole carbon source. Cells of Tolumonas lignolytica BRL6-1T are mesophilic, non-spore forming, Gram-negative rods that are oxidase and catalase negative. The genome for this isolate was sequenced and returned in seven unique contigs totaling 3.6Mbp, enabling the characterization of several putative pathways for lignin breakdown. Particularly, we found an extracellular peroxidase involved in lignin depolymerization, as well as several enzymes involved …
Long-Term Forest Soil Warming Alters Microbial Communities In Temperate Forest Soils, Kristen Deangelis, Grace Pold, Begüm D. Topçuoğlu, Linda T.A. Van Diepen, Rebecca M. Varney, Jeffrey L. Blanchard, Jerry Melillo, Serita D. Frey
Long-Term Forest Soil Warming Alters Microbial Communities In Temperate Forest Soils, Kristen Deangelis, Grace Pold, Begüm D. Topçuoğlu, Linda T.A. Van Diepen, Rebecca M. Varney, Jeffrey L. Blanchard, Jerry Melillo, Serita D. Frey
Microbiology Department Faculty Publication Series
Soil microbes are major drivers of soil carbon cycling, yet we lack an understanding of how climate warming will affect microbial communities. Three ongoing field studies at the Harvard Forest Long-term Ecological Research (LTER) site (Petersham, MA) have warmed soils 5°C above ambient temperatures for 5, 8, and 20 years. We used this chronosequence to test the hypothesis that soil microbial communities have changed in response to chronic warming. Bacterial community composition was studied using Illumina sequencing of the 16S ribosomal RNA gene, and bacterial and fungal abundance were assessed using quantitative PCR. Only the 20-year warmed site exhibited significant …