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Polybrominated Diphenyl Ethers And Organochlorine Pesticides In Human Milk From Massachusetts, Usa, Kathleen Arcaro, B. Johnson-Restrepo, R. Addink, C. Wong, K. Kannan
Polybrominated Diphenyl Ethers And Organochlorine Pesticides In Human Milk From Massachusetts, Usa, Kathleen Arcaro, B. Johnson-Restrepo, R. Addink, C. Wong, K. Kannan
Kathleen Arcaro
Concentrations of polybrominated diphenyl ethers (PBDEs), and organochlorine pesticides (OCPs; DDTs, HCHs, CHLs, and HCB) were measured in human breast milk samples collected across Massachusetts, USA, in 2004. Seventeen PBDE congeners were found in the samples, ranging in concentration from 0.06 to 1910 ng g(-1) lipid wt. BDE-47 (2,2',4,4'-tetraBDE), BDE-99 (2,2',4,4',5-pentaBDE), and BDE-100 (2,2',4,4',6-pentaBDE) were the major congeners detected in breast milk samples. Overall mean (+/-SD) concentrations of DDTs, HCHs, CHLs, and HCB were 64.5 +/- 75, 18.9 +/- 19, 32.4 +/- 36, and 2.3 +/- 2.2 ng g(-1) lipid wt, respectively. Concentrations of PBDEs were strongly correlated with concentrations …
Pld1 Is Overexpressed In An Er-Negative Mcf-7 Cell Line Variant And A Subset Of Phospho-Akt-Negative Breast Carcinomas, Kathleen Arcaro, J.M. Gozgit, B.T. Pentecost, S.A. Marconi, C.N. Otis
Pld1 Is Overexpressed In An Er-Negative Mcf-7 Cell Line Variant And A Subset Of Phospho-Akt-Negative Breast Carcinomas, Kathleen Arcaro, J.M. Gozgit, B.T. Pentecost, S.A. Marconi, C.N. Otis
Kathleen Arcaro
We have used a novel variant of the human oestrogen receptor (ER)-positive MCF-7 cell line, TMX2-28, as a model to study breast cancer. TMX2-28 cells show no detectable levels of mRNA or protein expression for the ER and express basal cytokeratins (CKs) 5, 14, and 17. cDNA microarray comparison between TMX2-28 and its parent cell line, MCF-7, identified 1402 differentially expressed transcripts, one of which was, phospholipase D1 (PLD1). Using real-time RT-PCR, we confirmed that PLD1 mRNA levels are 10-fold higher in TMX2-28 cells than in MCF-7 cells. We next examined PLD1 expression in human breast carcinomas. Phospholipase D1 mRNA …