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Full-Text Articles in Life Sciences
A Highway For War And Peace: The Secretory Pathway In Plant-Microbe Interactions, Dong Wang, X Dong
A Highway For War And Peace: The Secretory Pathway In Plant-Microbe Interactions, Dong Wang, X Dong
Dong Wang
Secretion of proteins and other molecules is the primary means by which a cell interacts with its surroundings. The overall organization of the secretory system is remarkably conserved among eukaryotes, and many of the components have been investigated in detail in animal models. Plant cells, because of their sessile lifestyle, are uniquely reliant on the secretory pathway to respond to changes in their environments, either abiotic, such as the absence of nutrients, or biotic, such as the presence of predators or pathogens. In particular, most plant pathogens are extracellular, which demands a robust and efficient host secretory system directed at …
A High-Performance, Small-Scale Microarray For Expression Profiling Of Many Samples In Arabidopsis-Pathogen Studies, Masano Sato, Raka M. Mitra, John Coller, Dong Wang, Natalie W. Spivey, Julia Dewdney, Carine Denoux, Jane Glazebrook, Fumiaki Katagiri
A High-Performance, Small-Scale Microarray For Expression Profiling Of Many Samples In Arabidopsis-Pathogen Studies, Masano Sato, Raka M. Mitra, John Coller, Dong Wang, Natalie W. Spivey, Julia Dewdney, Carine Denoux, Jane Glazebrook, Fumiaki Katagiri
Dong Wang
Studies of the behavior of biological systems often require monitoring of the expression of many genes in a large number of samples. While whole-genome arrays provide high-quality gene-expression profiles, their high cost generally limits the number of samples that can be studied. Although inexpensive small-scale arrays representing genes of interest could be used for many applications, it is challenging to obtain accurate measurements with conventional small-scale microarrays. We have developed a small-scale microarray system that yields highly accurate and reproducible expression measurements. This was achieved by implementing a stable gene-based quantile normalization method for array-to-array normalization, and a probe-printing design …
A Genomic Approach To Identify Regulatory Nodes In The Transcriptional Network Of Systemic Acquired Resistance In Plants, Dong Wang, Nita Amornsiripanitch, Xinnian Dong
A Genomic Approach To Identify Regulatory Nodes In The Transcriptional Network Of Systemic Acquired Resistance In Plants, Dong Wang, Nita Amornsiripanitch, Xinnian Dong
Dong Wang
Many biological processes are controlled by intricate networks of transcriptional regulators. With the development of microarray technology, transcriptional changes can be examined at the whole-genome level. However, such analysis often lacks information on the hierarchical relationship between components of a given system. Systemic acquired resistance (SAR) is an inducible plant defense response involving a cascade of transcriptional events induced by salicylic acid through the transcription cofactor NPR1. To identify additional regulatory nodes in the SAR network, we performed microarray analysis on Arabidopsis plants expressing the NPR1-GR (glucocorticoid receptor) fusion protein. Since nuclear translocation of NPR1-GR requires dexamethasone, we were able …
A Comprehensive Structure–Function Analysis Of Arabidopsis Sni1 Defines Essential Regions And Transcriptional Repressor Activity, Rebecca A. Mosher, Wendy E. Durrant, Dong Wang, Jungi Song, Xinnian Dong
A Comprehensive Structure–Function Analysis Of Arabidopsis Sni1 Defines Essential Regions And Transcriptional Repressor Activity, Rebecca A. Mosher, Wendy E. Durrant, Dong Wang, Jungi Song, Xinnian Dong
Dong Wang
The expression of systemic acquired resistance (SAR) in plants involves the upregulation of many Pathogenesis-Related (PR) genes, which work in concert to confer resistance to a broad spectrum of pathogens. Because SAR is a costly process, SAR-associated transcription must be tightly regulated. Arabidopsis thaliana SNI1 (for Suppressor of NPR1, Inducible) is a negative regulator of SAR required to dampen the basal expression of PR genes. Whole genome transcriptional profiling showed that in the sni1 mutant, Nonexpresser of PR genes (NPR1)–dependent benzothiadiazole S-methylester–responsive genes were specifically derepressed. Interestingly, SNI1 also repressed transcription when expressed in yeast, suggesting that it functions as …