Life Sciences Commons^™

Two Essays On Whole Farm Modeling And Crop Marketing In Western Kentucky, Benjamin A. Martin

Theses and Dissertations--Agricultural Economics

This thesis is composed of two essays that investigate whole farm planning and crop marketing in western Kentucky. In the first essay, contracting decisions between food corn producers and a mill are analyzed to observe factors affecting the bushel amount farmers contract. Unbalanced panel data containing seven years’ worth of pricing and contract information are used with a fixed-effects model to generate parameter estimates and quantify their effect on bushels contracted. It was found that contract attributes, market condition, and relationship-specific assets had a significant effect on producers’ food corn contracting decisions. The second essay utilizes mixed-integer programming to optimize …

Coffee Market In China, Ran Zhu

Theses and Dissertations--Agricultural Economics

Although China is generally known as a tea-consuming country, both the production and consumption of coffee in China has seen double-digit growth in the last decade. This upward trend is projected to continue as long as investment is sustained and levels of disposable income continue to increase. In this rapidly expanding market, it is important for coffee producers and retailers in China to understand the preferences of emerging Chinese coffee consumers. Using survey data from Hubei and Zhejiang, China, we apply a Tobit regression model to analyze Chinese coffee consumers. Results show that the length of time consumers have been …

Characterization And Evaluation Of Androgen-Binding Protein, Sex Hormone-Binding Globulin, And Thyroxine-Binding Globulin In The Horse, Blaire O'Neil Fleming

Theses and Dissertations--Veterinary Science

The objectives of this study are to characterize two carrier proteins in the horse that significantly decrease in humans following anabolic androgenic steroid administration: sex hormone-binding globulin (SHBG) and thyroxine-binding globulin (TBG). For SHBG characterization, qPCR, RNA sequencing, and immunohistochemistry were performed on testes and equine livers. Free and total testosterone immunoassays were utilized to confirm the presence of a carrier protein in equine circulation. SHBG was detected in the testes using qPCR, RNA sequencing, and IHC, indicating the presence of the isoform androgen-binding protein (ABP). SHBG was not detected in any liver samples. Evidence of a carrier protein was …

Pcb Disruption Of Gut And Host Health: Implications Of Prebiotic Nutritional Intervention, Jessie Baldwin Hoffman

Theses and Dissertations--Pharmacology and Nutritional Sciences

Exposure to environmental pollutants poses numerous risk factors for human health, including increasing incidence of cardiovascular disease and diabetes. Persistent organic pollutants, such as polychlorinated biphenyls (PCBs) have been strongly linked to the development of these chronic inflammatory diseases and the primary route of exposure is through consumption of contaminated food products. Thus, the gastrointestinal tract is susceptible to the greatest levels of these pollutants and is an important facet to study.

The first two hypotheses of this dissertation tested that exposure to PCBs disrupts gut microbiota directly (in vitro) and within a whole body system. PCB exposure …

Mechanisms And Potential Therapy On Disrupted Blood Pressure Circadian Rhythm In Diabetes, Tianfei Hou

Theses and Dissertations--Pharmacology and Nutritional Sciences

Arterial blood pressure (BP) undergoes a 24-hour oscillation that peaks in the active day and reaches a nadir at night during sleep in humans. Reduced nocturnal BP fall (also known as non-dipper) is the most common disruption of BP circadian rhythm and is associated with increased risk of untoward cardiovascular events and target organ injury. Up to 75% of diabetic patients are non-dippers. However, the mechanisms underlying diabetes associated non-dipping BP are largely unknown. To address this important question, we generated a novel diabetic db/db-mPer2^Luc mouse model (db/db-mPer2^Luc) that allows quantitatively measuring of mPER2 protein oscillation …

Towards A Healthier Chocolate Formula Which Is Rich In Polyphenols And Low In Fat, Lakmani Tharaka Galaniha

Theses and Dissertations--Nutrition and Food Systems

Chocolate is a food that is craved by many in the world and healthy chocolates have become a current topic in the healthy eating trend. The healthiness of a chocolate depends mainly on the polyphenol, fat and sugar contents. Although the literature serves several interventions to reduce the fat content and total calorie content of chocolates, it does not provide many interventions that consider both improving the polyphenol and reducing the fat content simultaneously. Considering this gap in the literature, this research project sought to develop a chocolate that is both low in fat and high in polyphenol content, without …

Cultural Sensitivity In Cooperative Extension Nutrition Education Programming In Kentucky, Anna Caroline Durr

Theses and Dissertations--Nutrition and Food Systems

The purpose of this mixed methods study was to learn how cultural sensitivity was integrated and perceived in community nutrition education of an existing program offered by the University of Kentucky Cooperative Extension Service Nutrition Education Program. Data collection consisted of a quantitative cultural competence assessment survey tool used with nutrition education paraprofessionals (n=74), qualitative in-depth interviews of nutrition education paraprofessionals (n=8) and focus groups of Hispanic/Latino nutrition education program participants (n=39). Findings were focused in three areas; the cultural sensitivity needs of the program, the perception of nutrition education by participants and the training and professional development needs of …

The Effects Of Housing On Dairy Cow Comfort, Immune Function, Stress, Productivity, And Milk Quality, Matthew Richard Borchers

Theses and Dissertations--Animal and Food Sciences

Mastitis and milk quality affect every dairy farmer across the globe. Sand bedded freestalls are the industry standard for cow comfort, welfare, and the control of environmental mastitis. Compost bedded packs may be a viable alternative to the sand bedded freestall.

Compost bedded packs are maintained at a consistent level of moisture, nutrients, and aeration to favor compost microorganisms. Greater bacteria counts in bedding have traditionally been associated with increased mastitis rates and mastitis pathogens can be found in the pack and on the teats of cattle housed in even well managed compost bedded pack barns. In spite of this, …

The Mechanism Of Rna Interference In Arthropods, June-Sun Yoon

Theses and Dissertations--Entomology

RNA interference (RNAi) is a useful reverse genetics tool for investigation of gene function as well as for practical applications in many fields including medicine and agriculture. Due to the variability in RNAi efficiency, RNAi-based methods are currently being developed for controlling only coleopteran insects which are known to be amenable to RNAi. The first chapter of my thesis includes findings from research to investigate what are the factors that make coleopteran insects relatively more efficient in RNAi. I used Colorado potato beetle (CPB), Leptinotarsa decemlineata and its cell line (Lepd-SL1) as study models to identify genes that play key …

Emerald Ash Borer (Coleoptera: Buprestidae) Host Expansion; Trading An Optimal Host For Enemy Free Space?, David G. Olson

Theses and Dissertations--Entomology

Emerald ash borer (EAB), Agrilus planipennis (Coleoptera: Buprestidae), is an aggressive invader from Asia that has killed millions of trees in North America. Recently EAB has been documented developing in a novel host, white fringetree, Chionanthus virginicus. I evaluated larval performance in two common ash species and white fringetree by infesting excised bolts with emerald ash borer eggs. In addition I evaluated several plant characteristics to determine which most influence larval development. I also conducted choice and no choice assays using the classical biological control agent, Tetrastichus planipennisi (Hymenoptera: Eulophidae), to assess its ability to locate larval EAB in …

Vitamin D Works Through The Lipid Droplet Protein Plin2 To Augment Mitochondrial Function In Skeletal Muscle, David M. Schnell

Theses and Dissertations--Pharmacology and Nutritional Sciences

Vitamin D has been connected with increased intramyocellular lipid (IMCL) mitochondrial function in skeletal muscle. It is also shown to prevent lipotoxicity in several tissues, but this has not yet been examined in skeletal muscle. Perilipin 2 (PLIN2), a lipid droplet protein upregulated with vitamin D treatment, is integral to managing IMCL capacity and lipid oxidation in skeletal muscle. Increased lipid storage and oxidation is associated with increased tolerance to a hyperlipidic environment and resistance to lipotoxicity. Therefore, I hypothesized that vitamin D increases β-oxidation and lipid turnover though a PLIN2 mediated mechanism, thereby preventing lipotoxicity.

This hypothesis was divided …

Conservation Limnogeology And Benthic Habitat Mapping In Central Lake Tanganyika (Tanzania), Joseph S. Lucas

Theses and Dissertations--Earth and Environmental Sciences

Small scale protected zones are valuable for helping the health and productivity of fisheries at Lake Tanganyika (East Africa). Spatial placement of protected areas relies on accurate maps of benthic habitats, consisting of detailed bathymetry data and information on lake-floor substrates. This information is unknown for most of Lake Tanganyika. Fish diversity is known to correlate with rocky substrates in ≤ 30 m water depth, which provide spawning grounds for littoral and pelagic species. These benthic habitats form important targets for protected areas, if they can be precisely located.

At the NMVA, echosounding defined the position of the 30-m isobath …

Dairy Cattle Hoof Disease Costs And Considerations For Prevention, Karmella A. Dolecheck

Theses and Dissertations--Animal and Food Sciences

Lameness is considered one of the most important health and welfare issues in the dairy industry. Understanding the total cost per case of disease-specific lameness can help producers select better treatment, prevention, and control strategies for their herds. The first objective of our research was to calculate the costs associated with 3 lameness causing hoof diseases: digital dermatitis, sole ulcer, and white line disease. To accomplish this, a survey of hoof health professionals (hoof trimmers and veterinarians) was conducted to identify treatment related expenditures per case. Data from the hoof trimmer responses to the survey and previously published research were …

The Effect Of Early Life Photoperiod Manipulation On Cocaine-Induced Behavioral Sensitization In Male And Female Japanese Quail, Shannon Elizabeth Eaton

Theses and Dissertations--Psychology

Estrogens seem to play a role in the locomotor activating effects of cocaine. Japanese quail provide a good model for hormonal manipulation as alterations of their photoperiod controls hormone levels. The current study aims to examine the role of early life photoperiod manipulation in cocaine-induced behavioral sensitization in quail. It was expected that if quail were raised on a short photoperiod, they would have a reduction in gonadal hormones and this reduction in hormones would affect the acquisition of cocaine-induced behavioral sensitization. Quail were raised on an 8L:16D or a 16L:8D light cycle. Following 2 days of habituation, quail were …

Impact Of Physico-Chemical Properties Of Manufactured Nanomaterials On Plant Uptake And Trophic Transfer, Jieran Li

Theses and Dissertations--Toxicology and Cancer Biology

Large quantities of manufactured nanomaterials (MNM) are released into the environment by human activity each year. The entry of MNM into the terrestrial food webs, which has the potential for far-reaching impacts, begins with the uptake by plant species from the soil. These processes can be affected by MNM physico-chemical properties such as size, chemical composition, surface charge, etc., of which our knowledge is still incomplete. To bridge some of the gaps in our understanding of these processes and, specifically, to determine whether the physico-chemical properties of the MNM are predictive of their behavior in terrestrial food chains, we conducted …

Β-Catenin Regulation Of Adult Skeletal Muscle Plasticity, Yuan Wen

Theses and Dissertations--Physiology

Adult skeletal muscle is highly plastic and responds readily to environmental stimuli. One of the most commonly utilized methods to study skeletal muscle adaptations is immunofluorescence microscopy. By analyzing images of adult muscle cells, also known as myofibers, one can quantify changes in skeletal muscle structure and function (e.g. hypertrophy and fiber type). Skeletal muscle samples are typically cut in transverse or cross sections, and antibodies against sarcolemmal or basal lamina proteins are used to label the myofiber boundaries.

The quantification of hundreds to thousands of myofibers per sample is accomplished either manually or semi-automatically using generalized pathology software, and …

Role Of Skeletal Muscle Mass In Sex-Dependent Power Output During Flywheel Resistance Training, Paul A. Baker

Theses and Dissertations--Kinesiology and Health Promotion

Background: To determine the role of muscle mass in sex-dependent differences in power output during flywheel resistance training (FRT).

Methods: Twenty recreationally active (≥ 2 resistance exercise bouts per week), subjects (10 M, 10 F) completed 2 bouts of resistance exercise using a flywheel resistance training (FRT) device (Exxentric kbox 4 Pro) separated by at least one week. Each session consisted of 3 sets of 4 exercises (squat, bent-over row, Romanian deadlift, and biceps curl) with varying moments of inertia (0.050, 0.075, and 0.100 kg/m², respectively) in random order. Each set consisted of 5 maximal effort repetitions with …

Bacterial Inoculants, Endophytic Bacteria And Their Influence On Nicotiana Physiology, Development And Microbiome, Andrea Marisa Sanchez Barrios

Theses and Dissertations--Plant and Soil Sciences

Soil and root microbial communities have been studied for decades, and the incorporation of high-throughput techniques and analysis has allowed the identification of endophytic/non-culturable organisms. This has helped characterize and establish the core microbiome of many model plant species which include underground and aboveground organs. Unfortunately, the information obtained from some of these model plants is not always transferable to other agronomic species. In this project, we decided to study the microbiome of the Nicotiana genus because of its importance in plant physiological and plant-microbe interactions studies. The data obtained was used as baseline information that allowed us to better …

Muscle Activation Analysis With Kinematic Comparison Between Wind-Up And Stretch Pitching With Respect To The Upper And Lower Extremities, Megan M. Smidebush

Theses and Dissertations--Kinesiology and Health Promotion

Introduction: Baseball pitching is considered one of the most intense aspects within the game of baseball, as well as the most complicated dynamic throwing task in all of sports. The biomechanics of pitching have been heavily investigated in an attempt to identify optimal pitching mechanics in terms of pitching performance. Previous quantified upper body kinetics research has concluded that improved muscle strength is needed in attempting to achieve adequate upper body kinetics and efficient pitching performances. Therefore, it is the purpose of this research study to compare the lower extremity muscle and upper extremity muscle activation patterns and kinematic variables …

Multivariate Analysis To Identify Potential Biomarkers For Prognosis And Treatment Resistance In Head And Neck Cancer Patients, Christina Ann Wicker

Theses and Dissertations--Toxicology and Cancer Biology

It is estimated that nearly 50,000 individuals in the United States will be diagnosed with head and neck cancer in 2017 (American Cancer Society www.cancer.org). Ninety percent of oral cancers are head and neck squamous cell carcinoma (HNSCC). Major obstacles in the treatment of HNSCC are recurrence and treatment resistance, which contributes to increased mortality. Therefore, there is increased need to determine genetic alterations in HNSCC that may be ideal novel drug targets, and biomarkers to improve diagnostic and prognostic testing.

Abnormal localization and overexpression of base excision repair protein and transcriptional regulator Apurinic/Apyrimidic endonuclease (APE1) has been associated with …

Initial Assessment And Effects Of Snake Fungal Disease On Populations Of Snakes In Kentucky, Jennifer Mckenzie

Theses and Dissertations--Forestry and Natural Resources

Pathogenic fungi are increasingly associated with epidemics in wildlife populations and represent a significant threat to global biodiversity. Snake fungal disease is an emerging disease caused by the fungus, Ophidiomyces ophiodiicola, and appears to be widespread in the eastern United States. Yet an evaluation of field diagnostics, and an understanding of the population-level consequences of the disease, are lacking. First, I evaluated the use of clinical signs to predict the presence of O. ophiodiicola across season and snake habitat affiliation (aquatic or terrestrial) and I compared two sampling methods to see if collection method impacts PCR result. Overall, snakes with …

Diet Composition Explains Reductions In Stream Salamander Occupancy And Abundance Along A Conductivity Gradient, Jacob Matthew Hutton

Theses and Dissertations--Forestry and Natural Resources

Changes in land use such as mountaintop removal mining with valley fills (MTR/VF) affect chemical, physical, and hydrological properties of headwater streams. Although numerous stream taxa have experienced significant declines from MTR/VF, stream salamanders appear to be particularly sensitive. Yet, the specific mechanism(s) responsible for the population declines has eluded researchers. We sampled salamander assemblages across a continuous specific conductivity (SC) gradient in southeastern Kentucky and estimated occupancy rates and abundance estimates along this gradient. We also examined the diet of larval and adult salamanders to determine if autochthony (A/T prey), total prey volume, and body condition is influenced by …

Assessing Ecosystem Services From The Forestry-Based Reclamation Of Surface Mined Areas In The North Fork Of The Kentucky River Watershed, Kumari Gurung

Theses and Dissertations--Forestry and Natural Resources

Land Use Land Cover (LULC) changes can take place at the expense of degrading environmental conditions and undermining ecosystem’s capacity to deliver benefits to people. In the Appalachian region, surface mining for coal is a major driver of LULC change. The Surface Mining Control and Reclamation Act (SMCRA) of 1977 requires mine site reclamation but typical reclamation practices often result in land cover dominated by grass and shrubs. The Forestry Reclamation Approach (FRA) is a promising reclamation strategy but not in widespread use by industry. Assessing ecosystem services that can be obtained from a forest landscape may help policy-makers and …

Effects Of Endophyte-Infected Tall Fescue Seed And Bromocriptine On Endocrine And Immune Function In Horses, Jessica Marie Hanneman

Theses and Dissertations--Veterinary Science

Consumption of endophyte-infected (E+) grasses has long been associated with health problems in animals. In cattle E+ tall fescue consumption leads to fescue toxicosis, and in horses it leads reproductive problems. The health-related issues associated with endophyte consumption have been attributed to the effects caused by the ergot alkaloids produced by the fungus. These ergot alkaloids are considered D2-like receptor agonists, and 5-HT2 serotonin and α-adrenergic receptor partial agonists. Many studies in humans, swine, cattle, and horses have identified that ergopeptines cause a decrease in prolactin production due to their dopaminergic activities. Additionally, these molecules have been found to cause …

Potential Candidates For Treating Deficits Associated With Developmental Ethanol Exposure In A Rodent Model: Solidago Nemoralis & Dimethoxybenzylidene-Anabasine, Logan James Fields

Theses and Dissertations--Psychology

Prenatal alcohol exposure (Fetal Alcohol Syndrome [FAS] and Fetal Alcohol Spectrum Disorders [FASD’s]) represents the leading preventable cause of intellectual disabilities in the western world, with FASDs estimated to affect approximately 2-5% of live births in the United States at an approximate annual cost of $3.6 billion (CDC, 2015; May et al., 2009). Ethanol (ETOH) exposure during development can lead to a variety of long-term behavioral impairments including problems with executive functioning, motor coordination, spatial learning, attention, and hyperactivity (Jones, 2011; Mattson & Riley, 1998). Much research has been conducted to develop pharmacological and/or environmental interventions to reduce these deficits, …

On-Farm Utilization Of Precision Dairy Monitoring: Usefulness, Accuracy, And Affordability, Elizabeth A. Eckelkamp

Theses and Dissertations--Animal and Food Sciences

Precision dairy monitoring is used to supplement or replace human observation of dairy cattle. This study examined the value dairy producers placed on disease alerts generated from a precision dairy monitoring technology. A secondary objective was calculating the accuracy of technology-generated disease alerts compared against observed disease events. A final objective was determining the economic viability of investing in a precision dairy monitoring technology for detecting estrus and diseases.

A year-long observational study was conducted on four Kentucky dairy farms. All lactating dairy cows were equipped with a neck and leg tri-axial accelerometer. Technologies measured eating time, lying time, standing …

Effectiveness Of A Physical Activity Enhancing Program For Use During Indoor Recess, Cassandra M. Blase

Theses and Dissertations--Kinesiology and Health Promotion

Purpose: The purpose of this study was to determine if a novel physical activity intervention game (Bingocize®) designed for use in confined spaces and modified to include age appropriate activities would increase the time spent engaged in physical activity (PA) during indoor recess. Methods: Fifty-two third grade children wore triaxial accelerometers during three different recess conditions. The recess conditions included: “typical” indoor recess (TIR), indoor recess with children engaged in Bingocize® (IRB), and “typical” outdoor recess (TOR). Results: There were significant (p < 0.05) differences among the recess conditions for the time spent in sedentary, light, moderate-to-vigorous physical activity (MVPA) intensity categories, as well as PA counts, and steps. During IRB, TIR, and TOR the subject were sedentary 42.4% (±0.10), 71.9%(±0.10), and 17.5% (±0.10) of the recess time, respectively. During IRB, TIR and TOR the subjects spent 43.4%(±0.10), 18.5%(±0.10), and 74.2%(±0.12) engaged in MVPA, respectively. Conclusions: These results indicate that Bingocize® promotes increased PA during times when inclement weather necessitates indoor recess and larger space requirements for activity are not available.

Alterations In Gabaergic Nts Neuron Function In Association With Tle And Sudep, Isabel Diane Derera

Theses and Dissertations--Physiology

Epilepsy is a neurological disorder that is characterized by aberrant electrical activity in the brain resulting in at least two unprovoked seizures over a period longer than 24 hours. Approximately 60% of individuals with epilepsy are diagnosed with temporal lobe epilepsy (TLE) and about one third of those individuals do not respond well to anti-seizure medications. This places those individuals at high risk for sudden unexpected death in epilepsy (SUDEP). SUDEP is defined as when an individual with epilepsy, who is otherwise healthy, dies suddenly and unexpectedly for unknown reasons. SUDEP is one of the leading causes of death in …

Vascular Cognitive Impairment And Dementia: The Importance Of Mixed Pathologies From Mouse Models To Humans, Alex Marian Helman

Theses and Dissertations--Molecular and Cellular Biochemistry

Age-related neurologic disease is a significant and growing burden on our society. Although the largest share of research effort has typically been devoted to the common neurodegenerative illnesses (such as Alzheimer’s disease, or AD), the reality is that nearly all cases of neurodegenerative disease possess elements of mixed pathology. Vascular contributions to cognitive impairment and dementia (VCID) is a complex form of dementia, combining aspects of vascular disease and other forms of dementia, such as Alzheimer’s disease. This pathology is heterogeneous and can include cerebral amyloid angiopathy (CAA), hemorrhages, white matter infarcts, and changes to the neurovascular unit. Given the …

Corn Grain Yield Components And Nutrient Accumulation In Response To Nitrogen, Plant Density And Hybrid, Maria Julia Santoro

Theses and Dissertations--Plant and Soil Sciences

Modern maize hybrids exhibit higher yields, increased biomass production, stress tolerance and greater nitrogen (N) use efficiency. Increased biomass accumulation can influence nutrient uptake and lead to increased nutrient removal. Hybrids were tested at seeding rates (SR) of 74000 (low) and 148000 (supraoptimal) plants ha^-1 and at N rates of 0 (deficient) and 390 (non-limiting) kg N ha^-1. Plants were sampled at V7, V14, R3, R5 and R6 and separated into vegetative and reproductive fractions for determination of dry matter and N accumulation. Grain yield was harvested at R6.

The high SR and high N treatment combination …