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Gene Expression Profiling In Winged And Wingless Cotton Aphids, Aphis Gossypii (Hemiptera: Aphididae), Xiaowei Yang, Xiaoxia Liu, Xiangli Xu, Zhen Li, Yisong Li, Dongyan Song, Tian Yu, Fang Zhu, Qingwen Zhang, Xuguo Zhou

Entomology Faculty Publications

While trade-offs between flight capability and reproduction is a common phenomenon in wing dimorphic insects, the molecular basis is largely unknown. In this study, we examined the transcriptomic differences between winged and wingless morphs of cotton aphids, Aphis gossypii, using a tag-based digital gene expression (DGE) approach. Ultra high-throughput Illumina sequencing generated 5.30 and 5.39 million raw tags, respectively, from winged and wingless A. gossypii DGE libraries. We identified 1,663 differentially expressed transcripts, among which 58 were highly expressed in the winged A. gossypii, whereas 1,605 expressed significantly higher in the wingless morphs. Bioinformatics tools, including Gene Ontology, …