Life Sciences Commons^™

Plant Polyadenylation Factors: Conservation And Variety In The Polyadenylation Complex In Plants, Arthur G. Hunt, Denghui Xing, Qingshun Q. Li

Plant and Soil Sciences Faculty Publications

BACKGROUND: Polyadenylation, an essential step in eukaryotic gene expression, requires both cis-elements and a plethora of trans-acting polyadenylation factors. The polyadenylation factors are largely conserved across mammals and fungi. The conservation seems also extended to plants based on the analyses of Arabidopsis polyadenylation factors. To extend this observation, we systemically identified the orthologs of yeast and human polyadenylation factors from 10 plant species chosen based on both the availability of their genome sequences and their positions in the evolutionary tree, which render them representatives of different plant lineages.

RESULTS: The evolutionary trajectories revealed several interesting features of plant polyadenylation factors. …

Utility Of Phylloplanins As Antibiotics, Selective Fungicides And For Enhancing Microbial Resistance In Plants, George Wagner, Ryan Sheperd

Plant and Soil Sciences Faculty Patents

The present invention relates to plant proteins produced by a plant's epidermal layer that contribute to the innate pest/disease resistance of the plant (“phylloplanins”), compositions comprising the phylloplanins and methods of using them. In particular the methods relate to inhibiting or preventing microbial, e.g., fungal or bacterial, growth on a subject, organism or surface by administering a phylloplanin. The invention also relates to transformed host cells that produce phylloplanins, and to transgenic plants producing phylloplanins conferring increased resistance to microbial infections/growth.

Plants Having An Enhanced Resistance To Necrotrophic Pathogens And Method Of Making Same, Pradeep Kachroo, Aardra Kachroo

Plant Pathology Faculty Patents

A method for enhancing resistance to necrotrophic and/or hemibiotrophic pathogens by overexpressing glycerol-3-phosphate dehydrogenase using an expression vector in a plant species. For example, the present method can be used to enhance resistance to C. higginsianum by overexpressing glycerol-3-phosphate dehydrogenase in a plant such as Arabidopsis plant, using an expression vector in a plant.

Development And Functional Analysis Of Novel Genetic Promoters Using Dna Shuffling, Hybridization And A Combination Thereof, Rajiv Ranjan, Sunita Patro, Bhubaneswar Pradhan, Alok Kumar, Indu B. Maiti, Nrisingha Dey

Kentucky Tobacco Research and Development Center Faculty Publications

BACKGROUND: Development of novel synthetic promoters with enhanced regulatory activity is of great value for a diverse range of plant biotechnology applications.

METHODOLOGY: Using the Figwort mosaic virus full-length transcript promoter (F) and the sub-genomic transcript promoter (FS) sequences, we generated two single shuffled promoter libraries (LssF and LssFS), two multiple shuffled promoter libraries (LmsFS-F and LmsF-FS), two hybrid promoters (FuasFScp and FSuasFcp) and two hybrid-shuffled promoter libraries (LhsFuasFScp and LhsFSuasFcp). Transient expression activities of approximately 50 shuffled promoter clones from each of these libraries were assayed in tobacco (Nicotiana tabacum cv. Xanthi) protoplasts. It was observed that most …

Gene Expression Modulation System For Use In Plants And Method For Modulating Gene Expression In Plants, Subba Reddy Palli, Ajay Kumar Singh

Entomology Faculty Patents

An EcR-based gene expression modulation system for use in plants includes an EcR gene expression cassette, a modified RXR gene expression cassette, and a gene-of-interest expression cassette, which can be expressed in a host plant cell.

Influence Of The Neotyphodium--Tall Fescue Symbiosis On Belowground Processes, D. H. Mcnear Jr., Rebecca L. Mcculley

Plant and Soil Sciences Faculty Publications

Much of the work to date on the relationships between cool season grasses and Neotyphodium fungal endophytes has focused on the physiological, biochemical, and genetic ramifications of the host-fungus relationship and the subsequent influence these effects have on ruminant nutrition, plant adaptation to environmental stresses, and aboveground ecological processes. Relatively little attention has been paid to effects on belowground parameters. In this paper, we review the research evaluating the impact of one endophyte-grass association, the Neotyphodium – tall fescue symbiosis, on underground ecological and biogeochemical processes. We also present some preliminary data showing that the quantity and nature of tall …