Life Sciences Commons^™

Promotor (Flt) For The Full-Length Transcript Of Peanut Chlorotic Streak Caulimovirus (Pclsv) And Expression Of Chimeric Genes In Plants, Indu B. Maiti, Robert J. Shepherd

Kentucky Tobacco Research and Development Center Faculty Patents

The isolation, modification and use of wild-type and modified viral FLt promoters of peanut chlorotic streak caulimovirus (PClSV) in the expression of chimeric genes in plant cells. The FLt promoter from PClSV has been modified to have duplicated enhancer domains.

Forage News [1998-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Hosts AFGC Board
• Forages at KCA
• Hay Testing is Best Bargain
• Historic Meeting is Approaching
• Monthly Alfalfa Hay Quality Awards to be Announced at Cave City
• Hard Seed in Alfalfa
• Forage Dependability
• 19^th Alfalfa Conference - March 4, 1999
• Upcoming Events

Comparison Of Water And Temperature Distribution Profiles Under Sand Tube Irrigation, Masoud Meshkat, Richard C. Warner, Stephen R. Workman

Biosystems and Agricultural Engineering Faculty Publications

Drip irrigation is one of the most efficient systems in delivering water to the plant root zone. Research has shown that the saturated, or nearly saturated, surface beneath the emitter may increase evaporation thereby reducing the irrigation efficiency. To increase the efficiency of surface applied drip irrigation on permanent tree crops a sand tube irrigation (STI) method was developed and tested. The sand tube method consists of removing a soil core beneath the emitter and filling the void with coarse sand. A weighing lysimeter was designed and instrumented to directly measure temporal evaporation during irrigation and for a period of …

Forage News [1998-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forage Conference a Success
• KFGC Award Winners
• Congratulations to Dr. Powell
• Landmark Event for Forages in Nebraska
• Hay Bales Don’t Always “Weigh-Up” to Expectations
• Minimize Losses in Hay Storage and Feeding
• Evening Grazing is More Efficient
• Roundup Ready Alfalfa?
• Endophyte in Perennial Ryegrass
• Preference by Ruminants Among Tall Fescue Cultivars
• Upcoming Events

Fecal Bacteria Survival And Infiltration Through A Shallow Agricultural Soil: Timing And Tillage Effects, C. S. Stoddard, Mark S. Coyne, John H. Grove

Plant and Soil Sciences Faculty Publications

Human and livestock exposure to fecal pathogens via contaminated surface or groundwater is an important water quality concern for soils receiving animal wastes. The effects of manure application timing (spring or fall application) and soil management (no-tillage or conservation tillage) on fecal bacteria infiltration through shallow karst soils in central Kentucky (the Bluegrass region) have not been evaluated. We performed a field experiment to measure fecal coliforms and fecal streptococci in leachate from dairy manure-amended no-tillage and conservation tillage soils. Manure significantly increased fecal bacteria in leachate compared with unmanured treatments. After manure application, the leachate that collected in zero-tension …

Viral And Insect Genes That Inhibit The Immune System And Methods Of Use Thereof, Bruce A. Webb, Liwang Cui

Entomology Faculty Patents

Viral, endoparasitoid and/or host genes that specifically inhibit the immune response of insect pests, useful for broadening the host range of insect viruses. Symbiont viruses of insect pests are genetically modified to express immune-suppressing proteins or biologically active fragments thereof and, optionally toxins, to increase the virus host range and/or improve the efficacy of insect pathogens.

Drosophila Unpaired Encodes A Secreted Protein That Activates The Jak Signaling Pathway, Douglas A. Harrison, Patricia E. Mccoon, Richard Binari, Michael Gilman, Norbert Perrimon

Biology Faculty Publications

In vertebrates, many cytokines and growth factors have been identified as activators of the JAK/STAT signaling pathway. In Drosophila, JAK and STAT molecules have been isolated, but no ligands or receptors capable of activating the pathway have been described. We have characterized the unpaired (upd) gene, which displays the same distinctive embryonic mutant defects as mutations in the Drosophila JAK (hopscotch) and STAT (stat92E) genes. Upd is a secreted protein, associated with the extracellular matrix, that activates the JAK pathway. We propose that Upd is a ligand that relies on JAK signaling to stimulate transcription of pair-rule genes in a …

Forage News [1998-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Fall Grazing School
• One Day Grazing Schools
• Forage Conference
• Forages at KCA
• Prussic Acid Poisoning
• Drought Stressed Pastures
• Upcoming Events

Response Of Runoff Diazinon Concentration To Formulation And Post-Application Irrigation, Joshua R. Evans, Dwayne R. Edwards, Stephen R. Workman, R. Michael Williams

Biosystems and Agricultural Engineering Faculty Publications

Pesticides used in urban environments can be transported in runoff to downstream waters and cause adverse environmental consequences. This experiment assessed the effects of post-application irrigation depth (0, 6.4, and 12.7 mm) and formulation (liquid and granular) on concentration and transport of diazinon (a pesticide commonly used for lawn insect control) in runoff from “tall” fescue (Festuca arundinacea Schreb.) plots. The post-application irrigation was applied using rainfall simulators immediately following diazinon application. The rainfall simulators were again used approximately 2 h after diazinon application to apply the equivalent of a heavy rainfall (64 mm/h for approximately 1.5 h) to generate …

Optimization Of Solid-State Fermentation Parameters For The Production Of Xylanase By Trichoderma Longibrachiatum On Wheat Bran, Elizabeth R. Ridder, Sue E. Nokes, Barbara L. Knutson

Biosystems and Agricultural Engineering Faculty Publications

Solid-state fermentation has the potential to produce inexpensive enzymes for use in high-volume industrial applications. Process parameters such as substrate moisture content and length of fermentation can have a significant effect on the amount and timing of enzyme production. This study was conducted in two stages, a screening stage and an optimization stage, to determine the effects of moisture content of the substrate, surfactant addition upon inoculation, depth of the substrate, and duration of fermentation on xylanase activity produced by Trichoderma longibrachiatum. Screening fermentations were conducted at 25°C, 50 and 75% wet basis moisture content (w.b.), 0.0 and 0.2% …

Forage News [1998-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Dollars & Cents of Stockpiling Tall Fescue
• KFGC Award Nominations Due October 9
• Kentucky Grazing School
• KFGC Fall Forage Conference
• Alfalfa Conference March 4
• Forages at the State Fair
• Maximize Grazing - Minimize Stored Feed
• Warm Season Grass Field Day
• Kentucky Pasture & Hay Conditions
• Forages at KCA
• Hay Quality Standards
• Upcoming Events

Vegetated Filter Strip Removal Of Cattle Manure Constituents In Runoff, Teng T. Lim, Dwayne R. Edwards, Stephen R. Workman, Brian T. Larson, Lloyd Dunn

Biosystems and Agricultural Engineering Faculty Publications

Pasture runoff can contribute to elevated concentrations of nutrients, solids, and bacteria in downstream waters. The objective of this study was to determine the effects of vegetative filter strip (VFS) length on concentrations and transport of nitrogen, phosphorus, solids and fecal coliform in runoff from plots treated with cattle manure. Three plots with dimensions of 2.4 × 30.5 m were used. The upper 12.2 m of each plot was treated with cattle manure, while the lower 18.3 m acted as a VFS. Runoff produced by rainfall simulators was sampled at VFS lengths of 0, 6.1, 12.2, and 18.3 m and …

Identification Of Putative Cytoskeletal Protein Homologues In The Protozoan Host Hartmannella Vermiformis As Substrates For Induced Tyrosine Phosphatase Activity Upon Attachment To The Legionnaires' Disease Bacterium, Legionella Pneumophila, Chandrasekar Venkataraman, Lian-Yang Gao, Subbarao Bondada, Yousef Abu Kwaik

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The Legionnaires' disease bacterium, Legionella pneumophila, is a facultative intracellular pathogen that invades and replicates within two evolutionarily distant hosts, free living protozoa and mammalian cells. Invasion and intracellular replication within protozoa are thought to be major factors in the transmission of Legionnaires' disease. We have recently reported the identification of a galactose/N-acetyl-d-galactosamine (Gal/GalNAc) lectin in the protozoan host Hartmannella vermiformis as a receptor for attachment and invasion by L. pneumophila (Venkataraman, C., B.J. Haack, S. Bondada, and Y.A. Kwaik. 1997. J. Exp. Med. 186:537–547). In this report, we extended our studies to the …

Forage News [1998-08], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Forages at the State Fair
• Late Summer Seeding Tips
• Applying Manure to Forage Crops
• Plan Now for Stockpiled Grass for Fall
• Upcoming Events

Quality Of Runoff From Plots Treated With Municipal Sludge And Horse Bedding, Elizabeth L. Busheé, Dwayne R. Edwards, Philip A. Moore Jr.

Biosystems and Agricultural Engineering Faculty Publications

Land application of horse stall bedding and municipal sludge can increase runoff concentrations of nutrients, organic matter, and bacteria as well as steroidal hormones such as estrogen. Concentrations of materials in runoff from sites treated with animal manure can be reduced by aluminum sulfate, or alum [Al₂(SO₄)₃•14H₂O] treatment. The objectives of this study were to assess plots treated with horse stall bedding or municipal sludge for: (a) runoff quality [concentrations of nitrate nitrogen (NO₃-N), ammonia nitrogen (NH₃-N), orthophosphate-phosphorus (PO₄-P), fecal coliform (FC), chemical oxygen demand (COD) …

Forage News [1998-07], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• U.K. All Commodity Field Day
• Fall Grazing School at Princeton
• Hay Seminar at the State Fair
• Native Warm Season Grass Field Day
• Forage Council Gets Grazing School Grant from Department of Agriculture
• Joint Field Day is the Cream of the Crop
• Prepare Now for Fall and Winter Pastures
• The Thirty Yard Journey
• Leaves of Grass
• Upcoming Events

Comparison Of Observed And Simulated Grow-Finish Swine Performance Under Summer Conditions, Larry W. Turner, Thomas C. Bridges, Richard D. Coffey, Richard S. Gates, Gary R. Parker, Tami M. Brown-Brandl, Douglas G. Overhults

Biosystems and Agricultural Engineering Faculty Publications

As a part of a National Pork Producers Council educational program, our research and extension team at the University of Kentucky was linked with an independent commercial swine producer to test the NCPIG model against observed commercial on-farm data. This experience provided improved information for model development as well as increased producer insight into the data input needs and potential benefits of modeling. Detailed production information comparisons between the NCPIG model and producer data are presented for summer time conditions to assess the validity of the NCPIG model for simulation of grow-finish swine performance. Results demonstrated that the NCPIG model …

A Role For Nima In The Nuclear Localization Of Cyclin B In Aspergillus Nidulans, L. Wu, S. A. Osmani, Peter M. Mirabito

Biology Faculty Publications

NIMA promotes entry into mitosis in late G2 by some mechanism that is after activation of the Aspergillus nidulans G2 cyclin-dependent kinase, NIMX^CDC2/NIME^{Cyclin B}. Here we present two independent lines of evidence which indicate that this mechanism involves control of NIMX^CDC2/NIME^{Cyclin B} localization. First, we found that NIME^{Cyclin B} localized to the nucleus and the nucleus-associated organelle, the spindle pole body, in a NIMA-dependent manner. Analysis of cells from asynchronous cultures, synchronous cultures, and cultures arrested in S or G2 showed that NIME^{Cyclin B} was predominantly nuclear during interphase, with maximal …

The Snare Machinery Is Involved In Apical Plasma Membrane Trafficking In Mdck Cells, Seng Hui Low, Steven J. Chapin, Christian Wimmer, Sidney W. Whiteheart, László G. Kömüves, Keith E. Mostov, Thomas Weimbs

Molecular and Cellular Biochemistry Faculty Publications

We have investigated the controversial involvement of components of the SNARE (soluble N-ethyl maleimide–sensitive factor [NSF] attachment protein [SNAP] receptor) machinery in membrane traffic to the apical plasma membrane of polarized epithelial (MDCK) cells. Overexpression of syntaxin 3, but not of syntaxins 2 or 4, caused an inhibition of TGN to apical transport and apical recycling, and leads to an accumulation of small vesicles underneath the apical plasma membrane. All other tested transport steps were unaffected by syntaxin 3 overexpression. Botulinum neurotoxin E, which cleaves SNAP-23, and antibodies against α-SNAP inhibit both TGN to apical and basolateral transport in …

Forage News [1998-06], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• U.K. All Commodity Field Day
• New Hay Publication Available
• Minimizing Losses in Hay Storage and Feeding
• Reducing the Risk of Hay Fires
• Attention KFGC Members!
• Determining Forage Moisture Content Using a Microwave Oven
• Upcoming Events

Diffuse Reflectance Changes During The Culture Of Cottage Cheese, Frederick Alan Payne, R. Carol Freels, Sue E. Nokes, Richard S. Gates

Biosystems and Agricultural Engineering Faculty Publications

A sensor for measuring diffuse reflectance of milk during the typical 6-h culture of cottage cheese was installed in a local manufacturing facility. Diffuse reflectance was found to increase slowly during the first three hours of the culture and increase rapidly toward the end of fermentation. The correlation between parameters generated from the diffuse reflectance profile and cutting time was sufficient to develop an algorithm for cutting time prediction. An algorithm incorporating t_max (time from adding culture to the maximum rate of change in reflectance) and slope of the reflectance curve at t_max predicted the operator selected cutting …

Forage News [1998-05], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Notes from the Kentucky Grazing School
• Beth Prewitt Wins Award at National Forage Meeting
• Balage is a Good Harvest Option
• Effect of Nitrogen on Legumes in Pastures
• Statewide Beef-Forage Field Day Planned
• Harvest Decisions Affect Quality
• Upcoming Events

Forage News [1998-04], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Henning Elected AFGC President
• CRP - Native Plant Establishment
• Influence of Soil Texture on Alfalfa Autotoxicity
• Grazing Management Effects on Yield and Persistence of Alfalfa
• Length of the Grazing Period: Does it Really Matter?
• Coated Alfalfa Seed Establishment and Yield Trials in Commercial Fields in Central Minnesota
• Alfalfa Hay Storage Losses as Influenced by Bale Type and Storage Method
• Upcoming Events

Cloning And Developmental Expression Of Pea Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit N-Methyltransferase, Robert L. Houtz

Horticulture Faculty Patents

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) εN-methyltransferase (protein methylase III or Rubisco LSMT) is disclosed. This enzyme catalyzes methylation of the ε-amine of lysine-14 in the large subunit of Rubisco. In addition, a full-length cDNA clone for Rubisco LSMT is disclosed. Transgenic plants and methods of producing same which (1) have the Rubisco LSMT gene inserted into the DNA, and (2) have the Rubisco LSMT gene product or the action of the gene product deleted from the DNA are also provided. Further, methods of using the gene to selectively deliver desired agents to a plant are …

Manipulation Of High Spatial Resolution Aircraft Remote Sensing Data For Use In Site-Specific Farming, Gabriel B. Senay, Andrew D. Ward, John G. Lyon, Norman R. Fausey, Sue E. Nokes

Biosystems and Agricultural Engineering Faculty Publications

Three spatial data sets consisting of high spatial resolution (1 m) remote sensing images acquired in 12 spectral bands, an on-the-go yield map, and a Digital Elevation Model were co-registered and evaluated for spatial variability studies in a Geographic Information Systems environment. Separate on-the-go yield maps were developed for 3, 5, and 12 statistically significant mean yield classes. For each yield class, the corresponding mean spectral and elevation data were extracted. The relationship between mean spectral and yield data was strongly linear (r = 0.99). Also, a strong linear relationship between mean yield and elevation data (r = 0.92) was …

Forage News [1998-03], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• National Alfalfa Symposium
• Statewide KCA/KFGC Field Day Planned
• Nitrogen for Early Grazing
• Christi Forsythe Receives Award
• Grazing School Update
• Round Bale Silage
• Variety Trial Information
• Contributions of Alfalfa to Wildlife and the Environment
• Planting Date, Fungicide, and Cultivar Effects on Sclerotinia Crown and Stem Rot Severity in Alfalfa
• Forage Quality Variation Among Maize Inbreds: In Vitro Fiber Digestion Kinetics and Prediction with NIRS
• Upcoming Events

Forage News [1998-02], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• National Alfalfa Symposium and XVIII Kentucky Alfalfa Conference February 26-27, 1998 University Plaza Hotel & Convention Center Bowling Green, Kentucky
• KFGC Awards to be Presented at Alfalfa Symposium
• U.K. All Commodity Field Day
• Grazing School for Spring 1998
• More Forage Learning Opportunities
• Forage Variety Reports
• Poultry Litter for Pastures
• Influence of Seedling Growth on Flooding Injury in Alfalfa
• Upcoming Events

Validating A Vegetative Filter Strip Performance Model, Puneet Srivastava, Thomas A. Costello, Dwayne R. Edwards, James A. Ferguson

Biosystems and Agricultural Engineering Faculty Publications

Vegetative filter strips (VFS) reduce losses of nutrients, solids, and other materials from land area treated with fertilizers and manures. A number of models are available that simulate nutrient and sediment transport in VFS. While VFS effectiveness is considered to depend on lengths of pollutant source area and VFS areas, few published studies have tried to validate these models using variable pollutant source area and VFS area. The objective of this study was to validate an event-based nutrient transport model (Chaubey et al., 1995) that simulates soluble nutrient transport in VFS. This model links three sub-models: modified Green-Ampt infiltration, non-linear …

Fecal Coliform Transport Through Intact Soil Blocks Amended With Poultry Manure, S. W. Mcmurry, Mark S. Coyne, E. Perfect

Plant and Soil Sciences Faculty Publications

Poultry production in Kentucky increased almost 200% between 1991 and 1995. Their waste is typically land applied, and fecal pathogen runoff and infiltration may cause nonpoint source groundwater pollution. We looked at the preferential flow of fecal coliforms through undisturbed soil blocks since fecal bacteria typically infiltrate the soil profile to contaminate groundwater. Poultry manure was uniformly distributed on top of sod-covered or tilled (upper 12.5 cm) soil blocks and the blocks were irrigated. Drainage was collected in 100 uniformly spaced cells beneath each block and analyzed for fecal coliform content and drainage volume. The spatial distribution of drainage and …

Forage News [1998-01], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Grazing Conference: Kickoff for KCA
• Kentucky Alfalfa Conference Joins National in 1998
• National Forage Conference in Indianapolis
• Benefits of Rotational Grazing
• Performance of Potato Leafhopper Resistance in Alfalfa During 1997
• Legume Seeding Rates for Renovating Grass Fields
• Upcoming Events