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Negative Regulatory Factors In Tombusvirus Replication: Known Proteins, Novel Roles, Paulina Alatriste González

Theses and Dissertations--Plant Pathology

Although host cells are a rather rich source for co-opted host factors, lipids and metabolites, positive stranded RNA viruses vastly rewire cellular pathways and remodel cellular membranes to support viral replication. To accomplish such major changes, these viruses depend on the availability of different host factors and the ability to readily assemble viral replication organelles (VROs). Genome-wide screens and proteomics approaches with Tomato Bushy Stunt Virus (TBSV) in a yeast model host indicated that tombusviruses rely on the cellular cytoskeleton to reorganize the cellular environment of their hosts. Using temperature-sensitive (ts) mutants of beta and gamma-tubulin proteins and pharmacological inhibitors, …