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Composition And Methods Of Using The Mirabilis Mosaic Caulimovirus Sub-Genomic Transcript (Sgt) Promoter For Plant Genetic Engineering, Indu B. Maiti, Nrisingha Dey
Composition And Methods Of Using The Mirabilis Mosaic Caulimovirus Sub-Genomic Transcript (Sgt) Promoter For Plant Genetic Engineering, Indu B. Maiti, Nrisingha Dey
Kentucky Tobacco Research and Development Center Faculty Patents
The isolation of and methods of using a sub-genomic transcript (Sgt) promoter from Mirabilis mosaic virus (MMV) are described. A 333 bp MMV Sgt promoter fragment (sequence−306 to +27 from the transcription start site, TSS) was found to be sufficient for strongest promoter activity. This MMV Sgt promoter fragment shows comparable promoter activity to the MMV FLt promoter both in transgenic plants and in protoplasts. The MMV Sgt promoter also demonstrates much greater activity compared to Cauliflower mosaic virus (CaMV) 19S promoter and 35S promoter. The MMV Sgt promoter fragment and any chimeric gene to which it may be linked …