Life Sciences Commons^™

Calibration Of A Water-Quality Model For Herrington Lake Using Empirically Derived Measurements Of Phytoplankton Growth And Nutrient Assimilation, Paul A. Bukaveckas, Angela S. Crain

KWRRI Research Reports

Importance of light limitation, nutrient availability, and hydrology in controlling the abundance and composition of the phytoplankton community of Herrington Lake (KY) was investigated over a two-year period. Selected environmental parameters were measured every two weeks (April-October) at five sampling stations located along the longitudinal gradient of the reservoir. In addition, short-term (48hr) nutrient enrichment experiments were conducted to assess the spatial and temporal variations in nutrient limitation. Phytoplankton growth responses to the combined addition of nitrogen (NO₃) and phosphorus (PO₄) were greater than those resulting from the addition of either nutrient alone. These results indicate …

Formulation Enhanced Transport Of A Soil Applied Herbicide, V. P. Evangelou, Louis M. Mcdonald, Sandra Prima

KWRRI Research Reports

Because pesticides are applied as formulated particles and the affinity of the active ingredient for the formulation is higher than for the bulk water, we hypothesized that a formulation complex could affect active ingredient transport. Our objectives were to investigate the nature and extent of surfactant-atrazine-clay/oxide surface interactions. When atrazine and an anionic surfactant were dried onto plain or Fe-coated sand and leached, atrazine concentrations in the initial leachate were lower in the Fe-coated sand treatment. This was likely due to an electrostatic attraction between the sand and surfactant. When a nonionic surfactant was used, atrazine concentration in the initial …

Metal-Resistance Genetically Engineered Bacteria, Sylvia Daunert, Donna Scott, Sridhar Ramanathan

KWRRI Research Reports

Bacterial-based electrochemical and optical sensing systems that respond in a highly selective and sensitive manner to antimonite and arsenite have been developed. This was accomplished by using genetically engineered bacteria bearing one of two plasmids constructed for our studies. The first plasmid, pBGD23, contains the operator/promoter region (O/P) and the gene of the ArsR protein from the ars operon upstream from the β-galactosidase gene. In the absence of antimonite/arsenite, ArsR binds to the 0/P site and prevents the transcription of the genes for ArsR and β-galactosidase, thus blocking expression of these proteins. When antimonite or arsenite is present in the …

Fish Lateral Line Neurophysiological And Neurobehavioral Responses As A Sensitive Water Quality Monitoring System, Hong Y. Yan

KWRRI Research Reports

Cadmium is a heavy metal ion that can cause deleterious effects on aquatic animals. This study uses both electrophysiological recordings from lateral line nerves and videotaping of schooling behavior to investigate the effects of cadmium exposure on fish. The fathead minnows were exposed to cadmium at a concentration of 450 μg/1 over a 24-hr period. Extracellular recording with a silver hook electrode was used to record compound action potentials from the lateral lines of control and experimental fish. After a short time exposure (24 hr) to cadmium ions, all of the electrophysiological activities of the lateral line nerves were suppressed. …

Potential For Crop Residue To Restrict Herbicide Movement In Surface From Water Corn And Soybean Fields, William W. Witt

KWRRI Research Reports

As no-tillage and other conservation tillage practices continue to increase, it is important to have knowledge of herbicide adsorption on crop residue with regard to the potential for the herbicide to be removed from the residue and move with runoff water from the field into nearby surface waters. Previous research had compared herbicide adsorption to various residues, but it was difficult to make comparisons among these studies because the residues were from different crops or the amount of residue decomposition was different. The amount of "weathering" or "aging" of the residue at the time of herbicide treatment could alter the …

A Survey Of Source And Finished Water Supplies For Giardia Cysts And Cryptosporidium Oocysts, Lisa Martin Sexton

KWRRI Research Reports

The occurrence of Giardia lamblia cysts and Cryptosporidium parvum oocysts was determined from source and treated drinking water supplies at selected water treatment facilities in Eastern Kentucky. Giardia and Cryptosporidium are two human protozoan parasites often found in water supplies due to fecal contamination. Characteristic cysts and oocysts were identified from large volume water samples, typically 100 gallons for raw samples and 1,000 gallons for finished water, using an indirect fluorescent antibody specific for Giardia and Cryptosporidium. Source water from four different water treatment facilities utilizing a reservoir, river, and two creeks were assayed. All sites exhibited Giardia lamblia …

Use Of Riparian Vegetated Filter Strips To Reduce Nitrate And Fecal Contamination In Surface Water, Mark S. Coyne, Robert L. Blevins, Rebecca A. Gilfillen

KWRRI Research Reports

This research assessed fecal bacteria trapping in surface runoff by grass filters and their potential to enhance NO₃^- removal via denitrification. Grass filter strips 9.0 m long trapped over 99% of the soil in surface runoff in 1992. Fecal coliform removal was less than 75%. In 1993, 9.0 and 4.5 m grass filter strips trapped 99 and 95% of the sediment, respectively. Fecal coliform trapping efficiency was 90% in 9.0 m grass filters and 75% in 4.5 m filters. Fecal streptococci trapping efficiency was 77% in 9.0 m grass filters and only 56% in 4.5 m filters. Fecal …

Teratogenic Effects Of Zinc On Embryo-Larval Stages Of The Fathead Minnow (Pimephales Promelas), Barbara A. Ramey

KWRRI Research Reports

The effects of zinc on embryos and larvae of the fathead minnow (Pimephales promelas) were studied using standard 8-day embryo-larval bioassay techniques. The objective was to determine if there was a period in embryonic development which would be the most sensitive to the toxic and teratogenic effects of zinc. Five developmental stages were exposed to 0.5, 1.0 and 3.0 mg/L. After 96 hr, all animals exposed to 3.0 mg Zn/L were dead. The hatching stage was the most affected by 1.0 mg/L, with only 59% surviving after 96 hr, while the tailbud stage showed essentially control-level survival. However, virtually all …

Potential Of Surface Water Contamination From Three Triazine Herbicides, William W. Witt, Kip W. Sander

KWRRI Research Reports

The movement of atrazine, cyanazine, and simazine from the site of application was monitored under conventional, reduced, and no-tillage conditions. Less water and soil was lost from the no-tillage and reduced tillage conditions. Conventional tillage conditions had about 66,000 L/ha runoff in 1986-87 and about 123,000 L/ha runoff in 1987-88. Seasonal rainfall was 885 mm in 1986-87 and 397 mm in 1987-88. The rainfall intensity was greater during the first two events in 1987 than the corresponding events in 1986. The first rainfall event in 1986 accounted for 91, 89, and 78% of the total seasonal loss of atrazine, cyanazine, …

Development Of Dynamic Non-Hortonian Watershed Models For Steeply Sloping Forested Watersheds: Application To Eastern Kentucky, Lindell E. Ormsbee, Abdul Q. Khan

KWRRI Research Reports

A comprehensive conceptual watershed model is developed to simulate the hydrologic response of steeply sloping forested watersheds. Two non-Hortonian and two Hortonian models were first tested with data from selected watersheds in West Virginia and eastern Kentucky in order to understand the different mechanisms of flow responsible for storm hydrograph generation in this type of watersheds. The two non-Hortonian models tested were the kinematic storage model (Sloan et al. 1983) and the saturation deficit model (Beven and Wood, 1983). Both models were unable to adequately reproduce the observed hydrographs in the four forested watersheds considered in this research. The two …

Effects Of Acid Ph On Embryonic And Juvenile Freshwater Fish, Barbara A. Ramey, Lee E. Colton

KWRRI Research Reports

The effects of sulfuric acid on embryos, larvae, and juvenile fish were examined using standard bioassay techniques, as well as in a preference/ avoidance behavioral test. The objectives were to compare the sensitivities of the various ages of fish to acid and to assess the use of the behavioral test in a hazard assessment program. In an 8-day static renewal bioassay, embryos and larvae of the fathead minnow were not affected at a pH of 4.92 and above, but pH 3.57 produced complete mortality prior to hatching. In 96- hr acute bioassays, 8-wk juvenile fathead minnows survived 100% at pH's …

Abatement Of Nitrate Pollution In Groundwater And Surface Runoff From Cropland Using Legume Cover Crops With No-Till Corn, M. Scott Smith, Jac J. Varco

KWRRI Research Reports

Agricultural practices can have a significant impact on water quality. The effects of leguminous winter cover crops on leaching of NO^-₃; from soil have been investigated in this project. Legume cover crops, by fixation of atmospheric N, can reduce the amount of fertilizer N required to produce summer grain crops. The methods initially used to evaluate cover crop effects on No; transport included suction probe lysimeters and measurement of NO^-₃; in soil samples collected to a depth of 90 cm. These measurements demonstrated extreme spatial variability in NO^-₃; distribution and water …

Toxicological Studies On Aquatic Contaminants Originating From Coal Production And Utilization: The Induction Of Tolerance To Silver In Laboratory Populations Of Fish And The Chronic Toxicity Of Nickel To Fish Early Life Stages, Wesley J. Birge, Jeffrey A. Black, James F. Hobson, Albert G. Westerman

KWRRI Research Reports

Aquatic toxicity studies were performed on two important coal-derived contaminants, silver and nickel. Silver was investigated with regard to metal-induced tolerance in laboratory populations of the fathead minnow (Pimephales promelas). Fish were exposed to acute silver concentrations following acclimation to sublethal exposures of this metal. Based on median lethal times (LT50), animals which had received 14 days prior exposure to 1.5 and 15 μg Ag/L were three to four times more resistant to silver than were previously unexposed organisms. This metal-induced resistance was not a sustained response. After organisms which had been acclimated to 15 μg/L had been transferred to …

Atlas Of Wetlands In The Principal Coal Surface Mining Region Of Western Kentucky, William J. Mitsch, Jan R. Taylor, Kimberly B. Benson, Paul L. Hill Jr.

KWRRI Research Reports

This atlas contains maps of wetlands and surface mining activity in the Western Kentucky Coal Field, and focuses on a 3960 km² (1530 mi²) region where approximately 90 percent of surface mining in the Coal field occures. Some present and potential competition exists between surface coal mining and wetland protection. A wetland classification, based on the recent FWS classification, includes six types of palustrine systems and one each of riverine and laucstrine systems. Wetlands and surface mines are located on twenty-seven 7.5 minute USGS quadrangles that define the study region. A total of 460 km² (177 …

Effects Of Surface Application Of Dairy Manure On The Infiltration Rate And Quality Of Surface Runoff, Joseph L. Taraba, I. J. Ross, John D. Bottom, Bill J. Barfield

KWRRI Research Reports

Dairy manure was surface spread on 12 ft x 12 ft plots on an established fescue pasture in the summer and fall of 1981 and 1982. The soil was a Maury silt loam. A simulated rainfall was applied to plots to test the effects of nitrogen loading rate (75, 150, and 300 #N/acre) time delay between manure application and the simulated rainfall events (0, 3, 6, 24, 48, 96 hours and a 120 hour test repeated on 0 hr plot with 300 #N/acre), and type manure (semi-solid - 1981 and liquid - 1982) on the concentrations of pollutants in the …

The Induction Of Tolerance To Heavy Metals In Natural And Laboratory Populations Of Fish, Wesley J. Birge, William H. Benson, Jeffrey A. Black

KWRRI Research Reports

Aquatic toxicity studies were performed on two natural populations of fathead minnows. One group of organisms was taken from a metal-contaminated flyash pond associated with a coal-fired power plant and the other group was collected from relatively uncontaminated hatchery ponds. Acute tests indicated that flyash pond fish were significantly more tolerant to cadmium and copper than were hatchery fish. At an exposure concentration of 6.0 mg Cd/L in moderately hard water, the median period of survival for flyash pond fish was 50.0 hr compared to 6.8 hr for hatchery fish. Both groups of organisms were about equally sensitive to zinc. …

Some Variations In Distribution Of Fishes In Large Mainstream Reservoirs Associated With Artificial Cover, Donald W. Johnson, Elizabeth M. Choinski

KWRRI Research Reports

The influence of artificial cover (brush piles) on fish populations in Kentucky Lake and Lake Barkley was studied. Mature and larval fishes were collected from deep and shallow sites with and without cover in a bay of each lake. Highest densities of mature crappie, bass, and sauger were found adjacent to deep attractors, while larval crappie and minnows were most concentrated at shallow brush piles. Shad (both adult and larvae) were not congregated at attractor sites. Information gathered supports the continuation of artificial cover installation and water level management procedures which will provide high and stable levels through spring spawning …

The Aquatic Toxicity Of Organic Compounds To Embryo-Larval Stages Of Fish And Amphibians, Jeffrey A. Black, Wesley J. Birge, Willaim E. Mcdonnell, Albert G. Westerman, Barbara A. Ramey, Donald M. Bruser

KWRRI Research Reports

Aquatic toxicity tests were conducted on 11 organic compounds considered hazardous to water resources. The toxicity of each compound was evaluated using embryo-larval stages of two to eight fish and amphibian species. Exposure was initiated at fertilization and maintained through 4 days posthatching. The animal test species exhibited varying degrees of sensitivity to the selected toxicants. Combined frequencies for mortality and teratogenesis at 4 days posthatching gave LC₅₀ ranges of 3.66 to 8.25 mg/L for benzene, 1.16 to 22.42 mg/L for carbon tetrachloride, 0.11 to 1.20 mg/L for chlorobenzene, 2.03 to > 68 mg/L for chloroform, 3.01 to 5.56 mg/L …

Sunlight Induced Movement Of Planktonic Organisms And Their Relationships To Water Movements, John Calkins, Ed Colley, Jay Wallingford, Carol Hulsey, Kathy Lohr, Marc Bolling

KWRRI Research Reports

Previous studies have shown the planktonic organisms of various natural water bodies to be quite sensitive to the levels of solar ultraviolet radiation incident at the water surface in summer. It has been assumed that the majority of the phytoplankton are simply carried about in the mass of the water which they inhabit with little control of their position or sunlight exposure. Photosynthetic microorganisms must strike a delicate balance with regard to solar radiation; they must obtain enough visible light for adequate photosynthesis while avoiding an excessive exposure to the injurious solar UV-B radiation.

It is proposed that the circulation …

Simulating The Water Requirements And Economic Feasibility Of Corn In The Midwest, Wendi L. Palmer, B. J. Barfield, M. E. Bitzer, C. T. Haan

KWRRI Research Reports

An evaluation of the economics of supplemental irrigation when using a surface water supply must be site specific in order to account for variations in soil moisture holding capacity, watershed area supplying the runoff, climatic conditions, and proposed irrigation management procedures.

With the use of farm specific simulation models to determine grain yields, availability of irrigation water, and economic expenditures involved in irrigation, an economic evaluation of supplemental irrigation can be performed, In the model presented in this report, the Duncan SIMAIZ model is used to predict grain yields using long-term daily weather information. SIMAIZ also determines irrigation water demand …

Effects Of Organic Compounds On Amphibian Reproduction, Wesley J. Birge, Jeffrey A. Black, Robert A. Kuehne

KWRRI Research Reports

Aquatic toxicity tests were conducted with atrazine, carbon tetrachloride, chloroform, methylene chloride, trisodium nitrilotriacetic acid (NTA), and phenol. Each compound was administered to developmental stages of three to five amphibian species. Exposure was initiated at fertilization and maintained through 4 days posthatching. Test responses included lethality and teratogenesis. Different amphibian species exhibited varying degrees of tolerance to the selected compounds. Greatest tolerance usually was observed for the more broadly adapted semi-aquatic and terrestrial species (e.g., Bufo americanus, Bufo fowleri). The more sensitive amphibians usually included those species which normally are restricted to aquatic or moist habitats (e.g., Rana …

Effects Of Polychlorinated Biphenyl Compounds And Proposed Pcb-Replacement Products On Embryo-Larval Stages Of Fish And Amphibians, Wesley J. Birge, Jeffrey A. Black, Albert G. Westerman

KWRRI Research Reports

Static renewal bioassays were performed with four polychlorinated biphenyls, including Capacitor 21 and Aroclors 1016, 1242, and 1254. Each compound was used to treat embryo-larval stages of the rainbow trout (Salmo gairdneri), channel catfish (Ictalurus punctatus), goldfish (Carassius auratus), redear sunfish (Lepomis microlophus), leopard frog (Rana pipiens), Fowler's toad (Bufo fowleri), and American toad (Bufo americanus). Developmental stages of the trout were the most sensitive, with LC₅₀ 's at 4 days posthatching of 0.3, 1.0, 1.1, and 1.6 μg/l for Aroclors 1254, 1242, 1016, and …

Influence Of Nitrogen Fertilization On The Quality And Quantity Of Streamflow From A Forested Watershed, George B. Coltharp, Michael T. Shearer, Everett P. Springer, Robert F. Wittwer

KWRRI Research Reports

This project was designed to determine the effects of nitrogen fertilization on the quality and quantity of streamflow eminating from an eastern hardwood forest watershed. A 40.67 ha watershed, located in mountainous eastern Kentucky, was aerially fertilized in late April 1975. The forest stand was principally oak, hickory, and yellow poplar, 50 - 55 years of age and in a relatively undisturbed condition. A helicopter applied anunonium nitrate at a rate of 504 kg/ha. Because a large part of applied nitrogen fertilizer ends up in the highly mobile nitrate nitrogen.form, this is the principal ion monitored in this study. No …

Studies On The Role Of Solar Ultraviolet Radiation In "Natural" Water Purification By Aquatic Ecosystems, John Calkins, Jeanne A. Barcelo, Perry Grigsby, Stephen Martin

KWRRI Research Reports

The possibility that pollution might deplete the stratospheric ozone layer and intensify solar UV at the earth's surface focuses attention on the role of solar UV in the various ecosystems at the earth's surface. Previous studies suggested that solar UV might contribute to bacterial die off in wastewater and the studies reported here were directed toward elucidating the action of solar UV in "natural" waters.

It has been assumed that solar UV action on aquatic ecosystems can be evaluated (using proper models) on the basis of the following four independently measurable quantities: I) the intensity of solar UV at the …

Biological And Chemical Evaluation Of The Aquatic Environment Of Selected Undeveloped Kentucky Lake Embayments, Marshall Gordon, Morgan E. Sisk

KWRRI Research Reports

This report describes research involving biological and chemical analysis of two undeveloped embayments on Kentucky Lake, namely Anderson and Vickers Bays. Field and laboratory studies were made to assess current biotic standing crops, limnological conditions, levels of inorganic and organic pollutants in the embayments.

A Study Of Naturally Occurring Algicides Produced By Freshwater Algae, Denny O. Harris, Harry D. Caldwell

KWRRI Research Reports

The mode of action of the algicide produced by Pandorina morum was examined by exposing Volvox globator and isolates spinach chloroplasts to a partially purified algicide preparation. Oxygen evolution of Volvox, whole chloroplasts and broken chloroplasts (minus the Calvin cycle),was reduced indicating that the algicide inhibits the light reactions of photosynthesis. Oxygen evolution studies of other Volvocaceae confirmed the observation that Pandorina morum is not significantly influenced by its own inhibitor. Molecular weight approximation by gel filtration established that the inhibitor has a low molecular weight (probably below 100 mw). Field studies indicate that this algicide has tremendous potential as …

Metabolic Capabilities Of Sulfur Oxidizing Bacteria And Their Role In Water Pollution, M. I. H. Aleem

KWRRI Research Reports

This report describes investigations into the physiology of microorganisms that are commonly involved in the oxidation of inorganic sulfur compounds. The metabolic activities of these bacteria play a potent role in several fields of economic importance such as strip mining operations, water pollution, corrosion, metallurgy, petroleum technology and soil fertility processes.

The oxidation of inorganic sulfur compounds was studied in the chemolithotrophs Thiobacillus denitrificans, Thiobacillus A₂, Thiobacillus neapolitanus, and a photolithotroph Rhodopseudomonas palustris. Cell suspensions from all of the thiobacilli catalyzed completely the oxidation of sulfide, thiosulfate, and sulfite to sulfate. The oxidation of thiosulfate in R. palustris …

Seasonal Changes In Water Quality And Primary Productivity In Doe Valley Lake, Edmond J. Bacon, Stuart E. Neff

KWRRI Research Reports

Primary productivity and water quality were studied in Doe Valley Lake, a 147-hectare impoundment on Doe Run, a spring-fed stream in Meade County, Kentucky, from 13 June 1969 to 31 July 1972. Doe Valley Lake is monomictic during most winter seasons, but it is dimictic during more severe winters because of its morphometry and location on the borderline climatic region for dimictic lakes (37° N latitude). Oxygen depletion in the hypolimnion is severe, and anaerobic conditions usually prevail by late June. A hypolimnetic areal deficit of 0.038 mg/cm²/day was calculated. Supersaturation of oxygen in the epilimnion was common, …

Development Of Prediction Relationships For Water Requirements With Irrigation Cooling, Billy J. Barfield, John N. Walker, F. A. Payne

KWRRI Research Reports

A model for predicting leaf temperatures during an off-on mist cycle is presented. The model uses a combination of energy budget and aerodynamic techniques. The model was tested for dry leaf temperature and evapotranspiration predictions using average hourly data from Arizona. The accuracy was good. It was also tested for dry leaf temperature predictions using two to three minute data with a widely varying net radiation. When reasonable values of stomatal resistance were used, the agreement was again good.

The model was tested for prediction of wet leaf temperature prediction with a ten minute on, fifty minute off mist irrigation …

A Study Of Water-Soluble Inhibitory Compounds (Algicides) Produced By Fresh-Water Algae, Denny O. Harris, Manhar C. Parekh

KWRRI Research Reports

A complex system of growth inhibitors was observed in the green algae (Volvocaceae). Inhibitors were found in the culture filtrates of some genera which limit their own growth (autoinhibitors) while others in the family produce substances which check the growth of other genera (heteroinhibitors). These inhibitors were destroyed by autoclaving. It was decided that Pandorina morum, which produced the strongest inhibitor and Volvox tertius, the most sensitive to the inhibitor would make an excellent model system for a study of the chemical and physical properties of these naturally occurring algicides. The algicide could be removed from actively growing cultures about …