Life Sciences Commons^™

Global Illumina Sequencing And The Development Of Est-Ssr Markers In Alfalfa, Zhipeng Liu, Yanrong Wang

IGC Proceedings (1997-2023)

RNA-Seq, a massively parallel sequencing method for transcriptome analysis, only analyzes transcribed portions of the genome. Recently, RNA-Seq has provided an opportunity to expand the identification of alfalfa (Medicago sativa) genes. Using Illumina sequencing, 124,025 unique sequences from MSGI 1.0 have been identified from the elongating stem and post-elongation stem internodes of two alfalfa genotypes (Yang et al. 2011). Using 454 sequencing, 54,216 unique sequences were obtained from the roots and shoots of two alfalfa genotypes (Han et al., 2011). In addition, Illumina sequencing of old and young stems of 27 alfalfa genotypes led to the …

Selection For Improved Saccharification Efficiency In Alfalfa Stems Assessed By Enzyme-Released Glucose, Annick Bertrand, Yves Castonguay, Réal Michaud, Marc-Olivier Duceppe

IGC Proceedings (1997-2023)

Alfalfa (Medicago sativa L.) has a high potential for sustainable bioethanol production, particularly because of its low reliance on N fertilizer (Samac et al. 2006). Genetic improvement for the accumulation of readily fermentable non-structural carbohydrates (NSC) and the saccharification of structural carbohydrate (SC) could significantly increase ethanol conversion rate. Genetic gains for these traits are tributary to the availability of screening techniques for the precise identification of superior genotypes with increased potential for the production of fermentable carbohydrates.

When assessing the genetic variability of parameters linked to cellulosic ethanol production (concentrations of NSC and SC), our results showed …

Physiological Characterization Of Cut-To-Cut Yield Variations Of Alfalfa Genotypes Under Controlled Greenhouse Conditions, Defeng Li, Baoluo Ma, Chengzhang Wang, Pufang Li

IGC Proceedings (1997-2023)

In a temperate region, alfalfa (Medicago sativa) crops are usually harvested 3-6 times per annum. The biomass yields of first and second cuts in the spring are generally the high-est. However, in subsequent cuts the biomass yields decline, with the final 1 or 2 cuts producing the lowest yields (Wang et al. 2009). This seasonal reduction in alfalfa biomass yields could be associated with prevailing changes in environmental factors such as rainfall and heat stress or due to biological characteristics of alfalfa crop itself. In this study, alfalfa was grown under controlled greenhouse conditions with suitable temperature, …

Perennial Legume Swards For Organic Farming System In Lithuania, Jonas Šlepetys, Alvyra Slepetiene

IGC Proceedings (1997-2023)

The economic and ecological benefits of forage legumes are well known. While perennial legumes can be useful in various aspects (Ledgard 2001), some legumes have short persistence in swards (Frame et al.1998; Vaiciulyte and Bacenas 2008) which is undesirable for organic farms where no mineral nitrogen is applied. Early flowering red clover (Trifolium pratense), which is the most common legume in Lithuania, has high yields only in the first – second years of use. In the third year, it often disappears completely.

In Lithuania, fodder galega (Galega orientalis) is the most long lived legume exhibiting …

Fodder Yield And Quality Of Lucerne (Medicago Sativa) Grown As Pure Stands And In Mixture With Oats (Avena Sativa), Mustard (Brassica Campestris) And Ryegrass (Lolium Perenne) Under Different Cutting Managements, Balwinder Kumar, U. S. Tiwana, Ajaib Singh

IGC Proceedings (1997-2023)

Legume-cereal mixtures are important protein and carbohydrate sources for livestock and provide a balanced chemical composition for livestock feeding along with higher green forage yields than the pure stands (Berdahl et al. 2001; Albayrak and Ekiz, 2005). Lucerne is the most important rabi legume crop of Punjab grown under limited irrigation conditions and provides succulent, palatable and nutritious fodder to dairy animals. The fodder yield of first cut of lucerne is very low so it is usually grown in mixture with oats and or mustard. The information on the mixtures of lucerne and suitable cutting times is scant. This …

Scenario Analysis: Risk And Return Of Aluminium Tolerant Lucerne, Claire D. Lewis, J. L. Jacobs, K. F. Smith, G. Spangenberg, B. Malcolm

IGC Proceedings (1997-2023)

Lucerne (Medicago sativa) yield is limited by aluminium stress associated with acid soils (Campbell et al. 1988; Scott et al. 2008). With the aid of transgenic technologies, the development of aluminium tolerant (Al Tol) lucerne is proposed. Modelled scenario analysis was conducted to explore the potential net benefits of Al Tol lucerne as part of a grazing system for a sheep production system in the high rainfall zone of south west Victoria.

Lucerne For Acid Soils: A Field Evaluation Of Early Generation Aluminium Tolerant Genotypes, Richard C. Hayes, Guangdi Li, Shoba Venkatanagappa, Alan W. Humphries, Ross Ballard

IGC Proceedings (1997-2023)

The development of lucerne germplasm tolerant of acidic soil conditions has long been a research objective of international significance. Many initiatives have previously failed to produce genotypes with adequate improvement in tolerance to be deemed economically viable and as a consequence, still no cultivar of lucerne exists that has improved adaptation to acidic soils. An Australian research program spanning the previous decade sought to redress this issue by developing lucerne genotypes with significantly enhanced tolerance to aluminium (Al) toxicity. Using recurrent selection in hydroponic solution culture, populations selected comprised individuals which displayed enhanced seedling root growth following a pulse of …