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The Effect Of Landlocking On Intestinal Aquaporin 1 Expression In Alosa Pseudoharengus (Alewife), Emily Funk
The Effect Of Landlocking On Intestinal Aquaporin 1 Expression In Alosa Pseudoharengus (Alewife), Emily Funk
Honors Scholar Theses
To determine the role of intestinal aquaporin 1 (AQP1), a passive water absorption channel, in the evolution of osmoregulatory physiology, I examined the differential expression of intestinal AQP1 between anadromous and landlocked ecotype of alewife in response to seawater challenges. I cloned and sequenced AQP1 from intestinal tissue of the alewife, following which I quantified the relative expression of AQP1 in each ecotype using Real-Time qPCR. In response to an acute seawater (30ppt) challenge, the anadromous alewives showed an upregulation of intestinal AQP1, while the landlocked alewives did not show a significant increased in AQP1 expression. After …