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Modulation Of Rho Termination : Expectation Vs Reality, Kavya Sri Sai Chegireddy
Modulation Of Rho Termination : Expectation Vs Reality, Kavya Sri Sai Chegireddy
Legacy Theses & Dissertations (2009 - 2024)
Transcription termination factor Rho is an essential protein in many bacteria. Rho is an ATP-dependent RNA helicase that accounts for 20-50% of termination events in Escherichia coli and plays an important role in preventing pervasive transcription that otherwise might be lethal to bacteria. Rho termination usually occurs in the 3’ UTRs and non-coding regions of the DNA, and the actively transcribing RNA is believed to be protected by translating ribosomes from Rho termination since both transcription and translation are a simultaneous process in prokaryotes. However, in reality, Rho terminates transcription of some protein-coding genes, and fails to terminate transcription of …
Epitranscriptomic Writer Regulation Of Gene Expression In Escherichia Coli, William Eugene Gasperi
Epitranscriptomic Writer Regulation Of Gene Expression In Escherichia Coli, William Eugene Gasperi
Legacy Theses & Dissertations (2009 - 2024)
The epitranscriptome is composed of enzyme catalyzed RNA modifications that can regulate tRNA, mRNA, and rRNA structure and function. RNA modifications can play key roles in regulating gene expression by controlling the when and how much of translation of specific transcripts. The SelU catalyzed tRNA wobble uridine (U) modification, occurs on tRNA lysine, glutamic acid, and glutamine. We have shown that E. coli deficient in selU gene are more sensitive to the antibiotic chloramphenicol (CAM) than their wild type counterparts. CAM works by inhibiting protein synthesis by binding to the ribosome and preventing peptide chain formation. We hypothesize that SelU …