Life Sciences Commons^™

Cloning And Sequencing Of Five Lea2 Genes From Date Palm Cv. Khalas And Functional Characterization To Heat And Salt Stress Tolerance Using Yeast Knockout Mutants, Mughair Abdul Aziz

Agribusiness Theses

Genomic assets from the plants innate to abiotic stress environments have the prospective to be latent in generating stress tolerant plants. Late embryogenesis abundant (LEA) proteins are decisive phytomolecules, which accumulates mainly in the late stages of seed development and in the vegetative tissues during exogenous stresses as a reaction to stabilize the damaged macromolecules. In spite of substantial research, their mechanism of action to initiate plant tolerance against abiotic stresses remains enigmatic. Here, the study is a preliminary investigation on LEA2 genes from the P. dactylifera cv. khalas, a relatively tolerant plant of arid regions. In this aspect, the …

Identification Of Inherited And De Novo Exomic Variations In An Emirati Family With Neurodevelopmental Disorders, Asmaa Samir Abdelaziz Refaey

Biology Theses

Neurodevelopmental disorders (NDDs) are a heterogenous group of disorders that affect children at any point of development and lead to mental and motor function deficits. Often, the underlying cause could be genetic and inherited. This study investigated possible genetic variations that could have led to these neurological abnormalities and other genetic disorders in an Emirati family. Whole exome sequencing (WES) was used to sequence the protein-coding regions of the genome to identify potential de novo and inherited variants that are associated with disorders in this family.

WES of DNA from the parents and ten children were performed. Several variants were …

Molecular And Physiological Assessment Of Salinity Stress Tolerance In Transgenic Arabidopsis Lines Expressing A Solanum Tuberosum Ribosome-Binding Protein, Onoud Rashed Saeed Ali Alyammahi

Biology Theses

Ribosomal proteins are highly conserved components of basal cellular organelles, primarily associated with translation of mRNA leading to protein synthesis. Additionally, some of these proteins are known to play critical role in plants RNA metabolism during stress responses, growth, and development. In this study, transgenic Arabidopsis plants expressing a ribosomal protein S27 (hereafter D26) isolated from Solanum tuberosum was subjected to NaCl-induced salinity stress conditions, to evaluate their putative stress resistance. Transgenic plants were exposed to high salinity stress, induced by 200 mM NaCl and physiological and biochemical assays were performed. The D26 transgenic plants demonstrated improved plant height and …

In Vitro Invistigation Of The Heterodimerization Between Angiotensin Ii And Thrombin Receptors, Isra Mansur Al Zamel

Biology Theses

The renin angiotensin system (RAS) through its hormone angiotensin (AngII) and the protease thrombin are two major physiological regulators of vascular and renal functions. RAS is involved in the regulation of blood pressure and water-electrolytes hemostasis, while thrombin has a crucial role in platelets activation and thrombosis. The interplay between these two systems at the physiological and the pathophysiological levels has been documented in many studies. However, such an interplay at the pharmacological level between the AngII and thrombin receptors still has not been explored. Our study aimed to explore the interplay between the AngII type 1 receptor (AT1R) and …

Evaluation Of The Genetic And Structural Variations Of Camel Hemoglobin, Amanat Ali

Dissertations

The single-humped Arabian camel (Camelus dromedarius) thrives in the hot arid Arabian desert. Many unique adaptations permit it to accomplish this. Camel erythrocytes or red blood cells (RBCs) have a peculiar elliptical shape and are amenable to large variations in physical conditions resulting from dehydration and rehydration cycles. The oxygen transport protein hemoglobin is found abundantly in RBCs and is also believed to behave differently in camels. While several physiological and biochemical studies have been performed on camel hemoglobin, very little is known about genetic and structural adaptions in this protein. The camel genome harbors several unique variations …

Hyperspectral Properties Of Date Palm Trees (Phoenix Dactylifera L.), Mohamed Ali Saeed Ahmed Al Abdouli

Theses

The goal of this study is to classify the Date Palm varieties based on hyperspectral signature technology since it is difficult to identify the Date Palm cultivars without fruits. It will also help to obtain the hyperspectral signature for different types of date palm trees. Moreover, it also assists to determine the wavelength fingerprint of each cultivar and to recommend the best classification protocol differentiating among different cultivars based on spectral signature. Utilizing the Hyperspectral imaging technology precisely on the leaves of different Date Palm cultivars, thus facilitating identification of date palm cultivars without the fruits and make the spatial …

Prevalence Of Brca1 And Brca2 Mutations Among Breast And Ovarian Cancer Patients In Northern Emirates, Zahra Ahmed Mohammed Saeed

Theses

Breast Cancer (BC) is the most common cancer and the second most cause of death among women. Mutations in BRCA1 and BRCA2 genes confer high susceptibility to both breast and ovarian cancer. However, data on the prevalence of the BRCA1/2 mutations among breast and ovarian cancer patients is limited. The genetic component of breast cancer in the UAE is largely unknown and no study has evaluated the BRCA mutations status in breast and ovarian cancer patients in the UAE population. This retrospective study aimed to establish mutation frequencies of the BRCA genes in breast and ovarian cancer patients from the …

Short Tandem Repeats Diversity In Indian And Pakistani Population Living In United Arab Emirates, Ruksar Salim Damji

Biology Theses

Short Tandem Repeats (STRs), have become increasingly popular markers of choice due to its wide array of advantages in the sector of forensic investigation. It is necessary to expand genetic research into residing populations i.e., Indian and Pakistani, in the same geographic region such as in the United Arab Emirates (UAE). The objectives of this study were to: (1) assess the forensic efficiency parameters and population structure analysis of the autosomal STR loci for the most potent amplification kit (2) estimate the allele frequencies; and (3) determine the significance of increasing the number of STR loci used in forensic DNA …

Study Of In Vitro Bioaccessibility And In Vivo Bioavailability Of Khalas Variety Date Seed Polyphenols For Functional Application In The Human Diet, Serene Hilary

Food Science Dissertations

Date seeds are an ideal source of polyphenols for the food industry. This doctoral work investigated Khalas variety date seeds in three forms, as powder (DSP), ethanolic extract (DSE) and functional food developed with the seeds; pita bread (DSB). The study characterised the polyphenol profile by mass spectrometry and investigated the bioaccessibility using simulated in vitro digestion coupled with Caco- 2 model for absorption and transport. It further explored the bioavailability of date seed polyphenols in vivo in humans by conducting a cross-over study in 16 healthy volunteers. The crossover study explored the urinary excretion of polyphenol metabolites, and the …

Ceullular Basis Of Missense Variants Causing Familial Hypercholesterolemia In Uae, Aseel Adnan Khalid Jawabri

Biochemistry Theses

Familial hypercholesterolemia is an autosomal dominant disorder characterized by elevated levels of low-density lipoprotein (LDL) carrying cholesterol in the blood. FH is mainly caused by mutations in the gene encoding the low-density lipoprotein receptor (LDLR) which causes altered lipid metabolism. LDLR is synthesized and initially modified by glycosylation in the endoplasmic reticulum (ER) and transferred to the plasma membrane by Golgi to carry out LDL clearance from the bloodstream. Defective LDLR can, therefore, lead to increased levels of LDL in the blood which may lead to atherosclerosis and premature coronary artery diseases.

For the past few years, cardiovascular diseases have …

Date Palm Wastes As Growth Substrate In Hydroponics To Grow Lettuce (Lactuca Sativa L.), Khawla Mohammed Al Marzooqi

Aridland Agriculture Theses

Recently, many countries are practicing a variety of modern farming techniques like hydroponics, aquaponics, aeroponics vertical farming etc., to enhance production and quality of the production. Among such techniques, hydroponic system of production is growing plants in an appropriate substrate, which provides both physical and nutrient support with a suitable root environment. Substrate media can be either inorganic or organic. In this study, date palm leaf waste has been used, rock wool slab and cocopeat slab. The main objective of this study is to investigate the feasibility of using palm leaf wastes as a growing substrate extensively in the hydroponics …

Expression Profiling And Mutant Analysis Reveal Complex Regulatory Networks Involved In Arabidopsis Response To Biotic, Abiotic And Hormonal Stresses, Arjun Sham Valiyaveettil Shamgopal

Biology Dissertations

Biotic and abiotic stress factors consistently threat during different stages of the plant’s life cycle. A complex network of signaling molecules is required to overcome stresses, which is linked to general physiological and molecular processes. Botrytis cinerea is a necrotrophic fungal pathogen that causes grey mold disease on many crops. Apart from B. cinerea, several biotic, abiotic and hormonal stresses have detrimental effects on plant growth and productivity. The aim of this study is to identify the genetic regulations of individual stress types using comparative microarray analyses, and to find out the role of differentially expressed genes in Arabidopsis- …

Prevalence, Antibiotic-Resistance And Growth Profile Of Vibrio Spp., Isolated From Imported Shellfish In The Local Markets, Hind Obaid Mohammed Al Rumaithi

Theses

Seafood-related human illness caused by Vibrio species is a major problem. Seafood is prone to contamination by pathogenic Vibrio bacteria especially, Vibrio mimicus, Vibrio parahaemolyticus, and Vibrio vulnificus. The study on the prevalence of this microorganism in seafood of the United Arab Emirates is vital due to the cultural background of the Emiratis as a coastal heritage. A study was conducted to assess the prevalence of Vibrio spp. in imported shellfish from local markets and to identify the Vibrio spp. examine the antimicrobial resistance and profile growth conditions of the isolated Vibrio. In the present study, …

Effect Of Mptp On Α-Synuclein Spreading, Accumulation And Toxicity In Mice With Intrastriatal Innoculation Of Human Α-Synuclein Preformed Fibril, Madiha Mohieldin Merghani

Theses

Parkinson’s disease (PD) is the second most common progressive neurodegenerative disorder, characterized by abnormal accumulation of interneuronal inclusions of α-synuclein (α-syn) known as Lewy bodies (LBs) and Lewy neurites (LNs), and significant loss of dopamine neurons in the substantia nigra pars compacta (SNc) region of the brain. Recent evidence suggests that intrastriatal inoculation of α-syn Preformed Fibril (PFF) into mice induces PD-like LBs and LNs pathology formed by aggregated α-syn in anatomically interconnected brain regions which needs several months of incubation to develop the pathology. In the present study, we have evaluated the effect of different doses of 1-methyl, 4-phenyl, …