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Full-Text Articles in Life Sciences
Disruption Of Rna Metabolism By Zika Virus, Maggie Lea Dickerson
Disruption Of Rna Metabolism By Zika Virus, Maggie Lea Dickerson
Honors Theses
Flaviviruses are positive, single-stranded, enveloped RNA viruses that are a part of the family, Flaviviridae. West Nile virus, Dengue, Zika virus and more are a part of this family. Mosquitoes are the vectors for these viruses. In order for the virus to infect mosquitoes, it must evade the RNA interference (RNAi), which is the major antiviral immune mechanism of insects. One study found the 3’ untranslated region (UTR) of the West Nile virus that inhibited the RNAi (GP et al. 2016). The goal of this study is to investigate if the 3’ and 5’ UTR region of the Zika virus …
Delivery Of Antiviral Sirna With Gold Nanoparticle Against Dengue Virus, Amanda J. Cooley
Delivery Of Antiviral Sirna With Gold Nanoparticle Against Dengue Virus, Amanda J. Cooley
Honors Theses
The goal of this study was to use specific small-interfering RNA (siRNA) sequences to target the E gene of the Dengue virus serotype 2 (DENV-2). It was predicted that this targeted binding of siRNA would prevent subsequent translation of the E gene protein, as well as virion production in infected cells. Due to the instability of the antiviral siRNA, the siRNA was conjugated to gold nano-particles (AuNP) in order to provide stability during delivery to the infected Vero cells. Cells were transfected with siRNA-AuNP complexes prior to DENV-2 infection. The siRNA-AuNP complexes tested included two different siRNA sequences (at varying …
Targeted Delivery Of Ribonucleotide Reductase Sirna In Cancer Cells Via Gold Nanoplexes, John Caleb Grenn
Targeted Delivery Of Ribonucleotide Reductase Sirna In Cancer Cells Via Gold Nanoplexes, John Caleb Grenn
Honors Theses
Cancer treatment has much room for improvement, as therapies today cannot differentiate well between what cells are part of a tumor and which are healthy. Our research involves targeting cancer cells by their overexpressed folic acid receptors, and delivering small interfering RNAs (siRNAs) to silence genes crucial to cell survival by the RNA interference gene knockdown pathway. By using Gaussia luciferase siRNA (siGLuc) as a model for cell delivery, we have been able to test our ability to deliver siRNA via a gold nanoparticle delivery system, also developed in our lab. We also synthesized ribonucleotide reductase siRNA (siRRM2), which will …