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Full-Text Articles in Life Sciences
Cloning And Expression Of Human Synaptosome Associated Protein 29 In E. Coli, Logan M. Ryals
Cloning And Expression Of Human Synaptosome Associated Protein 29 In E. Coli, Logan M. Ryals
Honors Theses
Acting as the chief mediators of vesicular fusion, soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) play a role in many intracellular trafficking events by moving opposing membranes into close proximity. One such event takes place in the process of autophagy. A key SNARE involved in autophagy is Synaptosome Associated Protein 29 (SNAP-29), which acts on the autophagosome membrane to promote autophagosome and lysosome fusion. Kaposi’s Sarcoma Herpesvirus (KSHV) proteins ORF33 and ORF38 were demonstrated to interact with SNAP-29. The exact mechanism of this interaction is yet to be elucidated but it is hypothesized that these interactions allow KSHV to modulate …
Amplification And Potential Transformation Of Human Syntaxin-17 Into Model E. Coli, Taylor H. Gore
Amplification And Potential Transformation Of Human Syntaxin-17 Into Model E. Coli, Taylor H. Gore
Honors Theses
Membrane fusion is key to organism homeostasis and occurs when a transport vesicle fuses with a target compartment, merging the two membranes into one while releasing the contents of the transport vesicle into the target compartment. This process is controlled by SNARE proteins. Syntaxin-17 protein plays a crucial role in the fusion of the autophagosome membrane with the lysosome membrane, allowing for degradation of misfolded intracellular components and pathogens. Intriguingly, Syntaxin 17 has been identified as a target of herpesvirus tegument proteins, although the pathological significance of the Syntaxin 17 and tegument protein interaction is unclear. As a first step …
Generation Of Mutant Snap-23 To Arrest Mast Cell Degranulation At Trans-Snare Complex Formation, Suzette Wafford-Turner
Generation Of Mutant Snap-23 To Arrest Mast Cell Degranulation At Trans-Snare Complex Formation, Suzette Wafford-Turner
Honors Theses
Regulated secretion of pro-inflammatory molecules (e.g, histamines, proteases) from mast cells plays critical roles in immunity, allergic reactions, cardiovascular disease and cancer. These molecules are stored in secretory granules inside the cell and are rapidly released into the extracellular environment when mast cells are activated. It is known that mast cell degranulation depends upon membrane anchored SNAREs (soluble N-ethylmaleidimide-sensitive factor attachment protein receptors) and accessory proteins that form the trans-SNARE complex, a 4 helical bundle central to exocytic fusion. There are three SNARE proteins that contribute to the 4-helical bundle during exocytosis; Syntaxin and VAMP proteins each provide one helix …
Subcloning And Expression Of Complexin Isoforms Involved In Mast Cell Degranulation, Cameron Blake King
Subcloning And Expression Of Complexin Isoforms Involved In Mast Cell Degranulation, Cameron Blake King
Honors Theses
Mast cells play an important role in the immune system by releasing chemicals such as chemokines and cytokines once they are stimulated. These products are released after stimulation by a process called mast cell degranulation. Mast cell degranulation is accomplished when vesicles containing the chemicals inside the mast cell fuse with the mast cell membrane via SNARE-mediated (Soluble NSF Attachment Protein Receptors) membrane fusion. This family of proteins consists of syntaxin, SNAP 25-like protein, and synaptobrevin/VAMP (Vesicle Associated Membrane Protein)(2). Comlexin isoforms (complexin 1,2,3,and 4) have been known to regulate this system in a fashion that is still unclear. In …