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Full-Text Articles in Life Sciences
Phosphodiesterase D Is Involved In Bile Resistance In Listeria Monocytogenes, Sophia A. Ali
Phosphodiesterase D Is Involved In Bile Resistance In Listeria Monocytogenes, Sophia A. Ali
Honors Theses
Listeria monocytogenes is a deadly foodborne bacterium that is responsible for almost 20% of food-related deaths in the United States. Listeria monocytogenes contaminates ready-to-eat products such as cheese, deli meat, and ice cream. Once ingested, it invades the intestinal lining and can enter the bloodstream, causing listeriosis. There is a gap in the knowledge of the pathogenesis of L. monocytogenes in how it is able to survive in the gastrointestinal tract in the presence of bile, which has bactericidal properties. Previous studies have suggested that the second messenger cyclic-dimeric-GMP may be involved in the regulation of virulence factors of Listeria. …
Assessing The Effect Of Bile And Oxygen Availability On The Redox Status Of Listeria Monocytogenes Strain F2365, Sukriti Bhattarai
Assessing The Effect Of Bile And Oxygen Availability On The Redox Status Of Listeria Monocytogenes Strain F2365, Sukriti Bhattarai
Honors Theses
Although bile is a bactericidal agent able to disrupt membrane structure and cellular homeostasis, including the induction of oxidative stress, Listeria can tolerate bile and also utilize it as a signal to enhance infection and virulence. Preliminary findings showed that under anaerobic conditions, exposure to bile significantly lowered the amount of oxidative damage present in bile-resistant strain F2365 cells. Similarly, Listeria further elicits an adaptive immune response, wherein pre-exposure of the bacterium to stress during food processing or in the host prior to entry into the intestine increases bile tolerance. Based on these previous studies, we hypothesized that bile induces …
The Incorporation Of Lipids Into The Cellular Membrane Of Salmonella, Betsy H. Redfern
The Incorporation Of Lipids Into The Cellular Membrane Of Salmonella, Betsy H. Redfern
Honors Theses
Salmonella is a gram negative, facultative anaerobic food borne pathogen and is the leading cause of deaths related to food borne illnesses. In order to establish an infection successfully, Salmonella must be able to survive in the presence of various stressors that it encounters, namely changes in pH, oxygen availability, osmolarity and bile. Previous research has shown that exposure to bile causes a shift in fatty acid composition in the cell membrane of the enteric bacterium Enterococcus faecalis. Thus, this led to the hypothesis that Salmonella incorporates fatty acids into its cellular membrane following exposure to bile and thereby protects …