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Full-Text Articles in Life Sciences

Restoring The Longleaf Pine (Pinus Palustris) Forests Using Pineywoods Cattle Grazing In Conjunction With Prescribed Burning, L. Tyler Albin May 2014

Restoring The Longleaf Pine (Pinus Palustris) Forests Using Pineywoods Cattle Grazing In Conjunction With Prescribed Burning, L. Tyler Albin

Honors Theses

The longleaf pine (Pinus palustris) is major forest constituent of the Southern Coastal Plains of the United States. Ecologically, a virgin longleaf pine forests supports increased species richness. Since the 1800s, longleaf pine forests have been exploited as a massive source of commercial products (e.g., lumber, pulp, and naval stores). A decrease in species richness has been recorded following this vast decrease in longleaf pine presence. Rebuilding the longleaf pine ecosystem is essential for restoring species richness and maintaining the ecological health of many Costal Plains habitats. Presently, the most popular restoration and management method utilized is prescribed burning. Prescribed …


Choice And Control Of Enrichment For A Rescued And Rehabilitated Atlantic Bottlenose Dolphin (Tursiops Trunactus), Hannah Bahe May 2014

Choice And Control Of Enrichment For A Rescued And Rehabilitated Atlantic Bottlenose Dolphin (Tursiops Trunactus), Hannah Bahe

Honors Theses

Animals living under human care experience stress related to a lack of control over t environment. One way to remedy this is through the use of cognitive enrichment, su choice and control of enrichment. This type of enrichment has been shown to have benefits for animals under human care including increased activity and social interactions. To determine if choice and control was more beneficial than enrichmen alone, a three year old male Atlantic bottlenose dolphin (Tursiops truncatus) was ex to novel enrichment items in two experimental conditions. For the first condition, th animal was given a novel object. During the …


Differences In Consumption Rates Between Juvenile And Adult Laccophilus Fasciatus Rufus (Coleoptera: Dytiscidae) On Larval Culex Quinquefasciatus (Diptera: Culicidae), Carmen E. Bofill May 2014

Differences In Consumption Rates Between Juvenile And Adult Laccophilus Fasciatus Rufus (Coleoptera: Dytiscidae) On Larval Culex Quinquefasciatus (Diptera: Culicidae), Carmen E. Bofill

Honors Theses

With the increase of global temperature and human populations, prevalence of vector-borne diseases is becoming an issue for public health. Over the years these vectors have been notorious for developing resistance to human regulated insecticides. Thus, other forms of control, including the use of natural predators, have become an important topic in research. Research on member of the family of predaceous diving beetles (Dytiscidae) and their predatory ability to decrease aquatic mosquito larvae populations has been of recent interest. The purpose of this study is to 1) quantify consumption rates of the dytiscid Laccophilus fasciatus rufus juvenile and adults on …


Subcloning And Expression Of Complexin Isoforms Involved In Mast Cell Degranulation, Cameron Blake King May 2014

Subcloning And Expression Of Complexin Isoforms Involved In Mast Cell Degranulation, Cameron Blake King

Honors Theses

Mast cells play an important role in the immune system by releasing chemicals such as chemokines and cytokines once they are stimulated. These products are released after stimulation by a process called mast cell degranulation. Mast cell degranulation is accomplished when vesicles containing the chemicals inside the mast cell fuse with the mast cell membrane via SNARE-mediated (Soluble NSF Attachment Protein Receptors) membrane fusion. This family of proteins consists of syntaxin, SNAP 25-like protein, and synaptobrevin/VAMP (Vesicle Associated Membrane Protein)(2). Comlexin isoforms (complexin 1,2,3,and 4) have been known to regulate this system in a fashion that is still unclear. In …


Cloning, Expression And Interaction Studies Of The Potential Rubisco Activase Cbbq, Salma A. Dawoud May 2014

Cloning, Expression And Interaction Studies Of The Potential Rubisco Activase Cbbq, Salma A. Dawoud

Honors Theses

Carboxysomes are polyhedral microcompartments found in all cyanobacteria and in many chemoautotrophs. Within their shell they contain the enzyme ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO), which fixes CO2. Downstream of the carboxysome operon there is another gene cluster containing several genes that may enhance carboxysome function. Two of these genes, cbbQ and cbbO, encode potential RubisCO activases. Using recombinant CbbQ and CbbO protein, and RubisCO isolated from carboxysomes the interaction between these proteins was studied. The CbbO and CbbQ proteins were both His tagged, allowing them to be purified with Ni2+-NTA column chromatography. Each of these tagged …


Carbon And Nitrogen Analysis To Determine Competitive Outcome For Three Species Of Container Mosquitoes, John Lloyd Martin May 2014

Carbon And Nitrogen Analysis To Determine Competitive Outcome For Three Species Of Container Mosquitoes, John Lloyd Martin

Honors Theses

It is well documented that intense larval competition exists between species of container mosquitoes. Two of the main genera of mosquitoes found to inhabit containers are Aedes and Culex. This study sought to determine the effects that different detritus treatments and larva ratios would have on carbon and nitrogen content, mass, and survival of larvae of various species. The species used in this experiment were Aedes albopictus, Aedes aegypti and Culex quinquefasciatus. I hypothesized that Ae. albopictus would be more efficient in acquiring nitrogen then the competitor species Ae. aegypti and Cx. quinquefasciatus. Thus, I expected Ae. albopictus to …


Regulation Of Gene Expression In Karenia Brevis, Skylar C. Rodgers May 2014

Regulation Of Gene Expression In Karenia Brevis, Skylar C. Rodgers

Honors Theses

Karenia brevis is species of dinoflagellate responsible for most of the harmful algal blooms that occur in the Gulf of Mexico. These blooms can be detrimental to the environment and the economy of a coastal region due to the brevetoxins produced by Karenia brevis. Currently, the cause of these blooms, as well as the mechanisms of associated toxin production, are unknown. Efforts to characterize Karenia brevis at a molecular level are ongoing. However, based on genomic findings, researchers have hypothesized that regulation of gene expression occurs post-transcriptionally. In many organisms, non-coding RNAs, such as natural antisense transcripts (NATs), play …


Self Myofascial Release: Effects On Hamstring Range Of Motion And Torque, Dillion F. Evans May 2014

Self Myofascial Release: Effects On Hamstring Range Of Motion And Torque, Dillion F. Evans

Honors Theses

Research has indicated that static stretching may reduce force production capabilities. This has led many practitioners to exchange static stretching for alternative methods of increasing range of motion (ROM) in warm-ups. Despite having little research to support its use, self myofascial release—foam rolling—has been suggested as a viable alternative (Macdonald et al, 2013). The objective of this study was to determine how self myofascial release (SMR) of the hamstring muscle group affects ROM and torque production capabilities of the hamstring muscle group. Ten subjects (age 26.5 6.5 years, mass 74.412.1 kg, height 1738 cm) were recruited. A within subjects randomized, …


Investigating The Co-Regulatory Role Of Midline And Extramacrochaetae In Regulating Eye Development And Vision In Drosophila Melanogaster, Lillian M. Forstall May 2014

Investigating The Co-Regulatory Role Of Midline And Extramacrochaetae In Regulating Eye Development And Vision In Drosophila Melanogaster, Lillian M. Forstall

Honors Theses

The Honors thesis research focused on the roles of extramacrochaetae and midline in regulating eye development and the vision of Drosophila melanogaster. It is known from previous studies that extramacrochaetae (emc) and midline (mid) independently regulate the formation of ommatidial units in the Drosophila compound eye. However, the thesis focuses on the interaction of these two genes and their co-dependent roles in regulating eye development. This study also attempts to explain the recovered formation of ommatidial units and interommatidial bristles when the expression of both of these genes is reduced and whether flies doubly mutant …


The Use Of A Small Molecule To Improve The Thermostability Of Dna Junctions, Arik Shams May 2014

The Use Of A Small Molecule To Improve The Thermostability Of Dna Junctions, Arik Shams

Honors Theses

The short-term goal of this research project is to employ small molecules as a means to stabilize four-way DNA junctions (4WJs) composed of natural DNA and chimeric nucleic acids. The long-term goal of the project is utilizing the 4WJs as extracellular therapeutic inhibitors of DNA binding proteins [i.e. Histones and High Mobility Group Protein B (HMGB1b)]. A number of studies have shown that classical intracellular DNA-binding proteins have a variety of deleterious side-effects when present in the extracellular milieu. In order to develop a successful 4WJ therapeutic, we are focused on using modified nucleic acids to enhance the stability of …


Study Of The Function Of The Mold Specific Gene Ms95 In Dna Repair In The Pathogenic, Dimorphic Fungus Histoplasma Capsulatum, Erin M. Smith May 2014

Study Of The Function Of The Mold Specific Gene Ms95 In Dna Repair In The Pathogenic, Dimorphic Fungus Histoplasma Capsulatum, Erin M. Smith

Honors Theses

Histoplasma capsulatum (Hc) is a dimorphic fungus that is the etiologic agent of the respiratory infection Histoplasmosis that is mediated by a shift from the mold phase to the pathogenic yeast phase. Genes have been identified that are specific to the mold or yeast phase in order to study the molecular biology of this shift. MS95 was identified in a subtractive cDNA library that was enriched for mold-specific genes, and has been found to be homologous to the DNA damage-responsive gene DDR48, which functions in DNA repair in Saccharomyces cerevisiae. In order to elucidate the function of MS95, a …


Role Of Il-17 In Cd8+ T Cell Mediated Clearance Of West Nile Virus, Jordan E. Lowery May 2014

Role Of Il-17 In Cd8+ T Cell Mediated Clearance Of West Nile Virus, Jordan E. Lowery

Honors Theses

West Nile virus (WNV), a positive sense single stranded RNA flavivirus, is prevalent in many countries worldwide and is a growing threat in the United States. Some patients infected with WNV develop severe neuroinvasive disease including meningitis or encephalitis which may lead to death. The recently characterized cytokine interleukin-17 (IL-17) has been implicated in several autoimmune diseases, neuroinflammatory conditions, and immune responses to various microbial infections. Previous research has indicated that a known contributor to IL-17 production, interleukin-23, is produced in response to WNV infection, but the role of IL-17 during WNV infection has not been studied before. This study …