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Articles 1 - 14 of 14
Full-Text Articles in Life Sciences
Assessing Prevalence Of Eating Disorders Among College Students, Taylor Jordan Duncan
Assessing Prevalence Of Eating Disorders Among College Students, Taylor Jordan Duncan
Honors Theses
Eating disorders have become a subject of concern for college-aged young adults in recent years. Risk factors like social media use are contributing to an increase in affected students, and research suggests that gender along with other variables plays a significant role. This thesis examined the current research on eating disorders and risk factors associated with their development. Through an online survey, information from freshman, sophomore, junior, senior, and graduate students at The University of Southern Mississippi regarding their current eating behaviors, feelings toward food and body, and demographic information was collected. Data collected indicated the student population’s level of …
Microrna Identification And Target Prediction In The Whitefly (Bemisia Tabaci), Alexis Aleman
Microrna Identification And Target Prediction In The Whitefly (Bemisia Tabaci), Alexis Aleman
Honors Theses
RNA interference, referred to as RNAi, is a biological phenomenon whereby knock-down of gene expression can be achieved through the use of RNA molecules, including small interfering RNAs (siRNAs) and microRNAs (miRNAs). The use of miRNAs is an endogenous pathway that results in the degradation of the miRNA strand’s complementary messenger RNA (mRNA), preventing the translation of the mRNA and therefore the production of the proteins necessary for gene expression. While RNAi is a biological phenomenon that occurs naturally, it is also a method that can be used and manipulated in the laboratory to try to control the expression of …
Cloning, Expression, And Purification Of Fadk And Its Application In Coa-Rna Capture, Jon-Michael L. Stork
Cloning, Expression, And Purification Of Fadk And Its Application In Coa-Rna Capture, Jon-Michael L. Stork
Honors Theses
Coenzyme A (CoA) is an important enzyme cofactor involved in acyl transfer reactions. Recently, CoA and its various thioesters were found to exist at the 5’ end of RNA molecules. Although the function of these molecules is still unknown due to difficulties in their isolation, their existence at the 5’ RNA end reveals potentially novel biological roles of RNA. We are exploiting the broad substrate tolerance of the medium chain fatty acid-coenzyme A ligase (FadK), hypothesizing that this enzyme will accept biotinylated medium chain fatty acid substrates, creating biotin-tagged CoA-RNA. This modified CoA-RNA can later be captured using streptavidin affinity …
The Dna Damage Response Gene Ddr48 Regulates Sterol Synthesis Genes To Confer Antifungal Resistance To The Pathogenic Fungus Histoplasma Capsulatum, Gabriella Reyes
The Dna Damage Response Gene Ddr48 Regulates Sterol Synthesis Genes To Confer Antifungal Resistance To The Pathogenic Fungus Histoplasma Capsulatum, Gabriella Reyes
Honors Theses
Histoplasma capsulatum is a dimorphic fungal pathogen that is endemic to the Ohio, Missouri, and Mississippi river valley regions. The fungus grows as a mold at environmental temperatures (25C) and transforms into a unicellular yeast upon inhalation by a mammalian host (37C). The mold to yeast shift is required for pathogenicity in host organisms, where the potentially fatal disease, histoplasmosis, can present. This study aims to characterize the DNA damage response protein DDR48 and the role that it plays in combating cellular stressors in H. capsulatum. We found that DDR48 is expressed strongly in the mold phase but expressed only …
Age-Related Tick Infestation Patterns In Springtime Migratory Passerines, Matthew Dunning
Age-Related Tick Infestation Patterns In Springtime Migratory Passerines, Matthew Dunning
Honors Theses
To test the hypothesis that younger migrants are more prone to arrive at stopover sites on the northern coast of the Gulf of Mexico with ticks than adult migrants, 2177 migratory passerines were screened for ticks at Gulf Coast sites in Texas and Louisiana. Fifty eight (2.7%) were infested with ticks with 28 (1.3%) being young birds and 30 (1.4%) being adult birds. The body condition of the birds was assessed to determine if tick infestation had negative consequences and if the severity of consequences varied between ages. I found no relationship of age with the prevalence of a tick …
The Incorporation Of Lipids Into The Cellular Membrane Of Salmonella, Betsy H. Redfern
The Incorporation Of Lipids Into The Cellular Membrane Of Salmonella, Betsy H. Redfern
Honors Theses
Salmonella is a gram negative, facultative anaerobic food borne pathogen and is the leading cause of deaths related to food borne illnesses. In order to establish an infection successfully, Salmonella must be able to survive in the presence of various stressors that it encounters, namely changes in pH, oxygen availability, osmolarity and bile. Previous research has shown that exposure to bile causes a shift in fatty acid composition in the cell membrane of the enteric bacterium Enterococcus faecalis. Thus, this led to the hypothesis that Salmonella incorporates fatty acids into its cellular membrane following exposure to bile and thereby protects …
The Acute Effects Of Artificially-Sweetened Beverages On Cardiovascular Health, Adria Mulligan
The Acute Effects Of Artificially-Sweetened Beverages On Cardiovascular Health, Adria Mulligan
Honors Theses
Consuming artificially-sweetened beverages instead of sugar-sweetened beverages is becoming increasingly popular among those who want to lose weight, have medical conditions that prohibit intake of sugar, or want to improve overall health. However, little research has been conducted on the repercussions of the intake of these diet drinks, specifically on how they affect the cardiovascular system. The purpose of this study was to examine the acute effects of artificially-sweetened beverages on cardiovascular health. Eight participants with no pre-existing heart conditions underwent two separate appointments, one where they consumed a 12 oz. water and one where they consumed a 12 oz. …
The Correlation Between Physical Activity And Grade Point Average, Asia Mccoy
The Correlation Between Physical Activity And Grade Point Average, Asia Mccoy
Honors Theses
The correlation between grade point average (GPA) and physical activity has typically been researched in adolescents, but not a lot of research has focused on traditional college students. Previous research in adolescents has shown mixed outcomes, but there is still evidence that a positive correlation exists between physical activity and GPA. It is also believed that adolescents who are physically active tend to have better academic outcomes than their non-active counterparts as they mature. The primary purpose of our study was to examine the correlation between GPA and physical activity in students attending the College of Education and Human Sciences …
Checklist Of The Vascular Flora, Restoration Plans, And Educational Interpretive Signs For A Remnant Prairie At The Lakewood Memorial Cemetery, Hinds County, Mississippi, Olivia Shelton
Honors Theses
A floristic survey was conducted on the eastern margin of the Lakewood Memorial Park Cemetery, located between Jackson and Clinton, Mississippi, near the western edge of the Jackson Prairie ecoregion. From soil map data and preliminary observations of the landform and flora, the eastern part of the cemetery was hypothesized to be remnant prairie now mostly covered by exotic grasses (pasture) and woodland. Plant specimens were collected, identified, and preserved in the herbarium of The University of Southern Mississippi (USMS). The survey yielded 119 species of vascular plants, of which 98 (82%) were native and 21 (18%) were non-native. Indicative …
Characterizing The Central Hemodynamic Response To Orthostasis: Influence Of Sex, Fitness And Body Composition, Benjamin Schreck
Characterizing The Central Hemodynamic Response To Orthostasis: Influence Of Sex, Fitness And Body Composition, Benjamin Schreck
Honors Theses
Inter-individual responses to orthostasis (i.e., ability to maintain consciousness in the upright posture) exist. However, few studies provide insight into the potential mechanisms for this variation. The purpose of this thesis project was to explore individual differences (i.e., sex, fitness, and body composition) on the central hemodynamic response to a modified head-up tilt table test (HUT). Fourteen volunteers with an average age of 22±1 years and an average body mass index (BMI) of 8±1 kg/m2 underwent assessments of pulse wave analysis, heart rate variability, and perfusion determination via near-infrared spectroscopy over the gastrocnemius muscle while supine, followed by a …
The Importance Of Rnd-Type Efflux Pumps In The Interactions Of Pseudomonas Aeruginosa With 4-Methoxybenzaldehyde, Sanchirmaa Namjilsuren
The Importance Of Rnd-Type Efflux Pumps In The Interactions Of Pseudomonas Aeruginosa With 4-Methoxybenzaldehyde, Sanchirmaa Namjilsuren
Honors Theses
Pseudomonas aeruginosa is a common hospital-acquired pathogen and is often associated with high mortality rates due to the development of multidrug-resistance. Antimicrobial plant-derived (phyto-) aldehydes present a promising alternative to antibiotics due to their broad-spectrum antimicrobial activity and low propensity to trigger resistance. However, two main problems preclude the widespread application of these compounds: chemical instability and low antimicrobial efficacy. The ongoing collaboration between the Mavrodi and Patton labs at USM has recently addressed the problem of chemical instability by incorporating plant aldehydes into polymer materials called Pro-Antimicrobial Networks via Degradable Acetals (PANDAs). Thus, this study aimed to address the …
Transformation Of Model Organisms With Munc13-1 For Subcloning And Protein Harvesting, Grace Dittmar
Transformation Of Model Organisms With Munc13-1 For Subcloning And Protein Harvesting, Grace Dittmar
Honors Theses
Exocytosis is the process by which cells release various molecules into the extracellular space. This mechanism is accomplished by membrane fusion, in which the membrane of the transport vesicle and that of the cell itself fuse to create one continuous membrane, allowing the release of the molecules inside the vesicle. A family of proteins, known as SNARE proteins, is responsible for facilitating membrane fusion. SNARE proteins form a complex between the vesicle membrane and the plasma membrane. For various cells, such as mast cells and neurons, exocytosis is essential to carry out their vital functions. Various accessory proteins are known …
The Impact Of Oxygen On The Intracellular Survival Of Listeria Monocytogenes, Amber N. Coats
The Impact Of Oxygen On The Intracellular Survival Of Listeria Monocytogenes, Amber N. Coats
Honors Theses
Listeria monocytogenes is an intracellular pathogen that is responsible for the foodborne disease listeriosis. In order to invade host cells and establish an infection, this pathogen must travel through the gastrointestinal tract where it is exposed to a variety of stressors, including low pH, bile, and variations in oxygen concentrations. These stressors necessitate this organism’s ability to sense and respond to its environment. Previous research has shown that bacteria use two-component systems to sense and regulate genes needed for improved survival in stressful environmental conditions. The goal of this study was to determine if the expression of the putative oxygen …
Fate Of Ingested Rna In The Two-Spotted Spider Mite, Obrie D. Scarbrough
Fate Of Ingested Rna In The Two-Spotted Spider Mite, Obrie D. Scarbrough
Honors Theses
RNA interference, or RNAi, is a gene regulation mechanism that uses small noncoding RNAs (sRNAs) to silence the expression of certain genes. The application of RNAi has been extended to insect pest control. The two-spotted spider mite Tetranychus urticae is a persistent agricultural pest that tends to develop pesticide resistance at an alarming rate, making it a perfect candidate for RNAi technology development. It was hypothesized that unique sRNAs could be isolated from RNA soaked spider mites, and new synthetic RNAs could be synthesized to elicit greater knockdown than was achieved in previous studies. To perform this research, a small …