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Articles 1 - 30 of 32
Full-Text Articles in Life Sciences
Assessing The Role Of The Microbiome, Parasite Infections, And Movement In Avian Health, Olivia N. Choi
Assessing The Role Of The Microbiome, Parasite Infections, And Movement In Avian Health, Olivia N. Choi
Electronic Theses and Dissertations
Avian health encompasses the physical, physiological, and behavioral well-being of birds. Assessing avian health is not only important for the conservation and management of wild birds and the recreational economy, but also for the management of infectious diseases that threaten public health and agriculture. Birds, comprising approximately 10,000 species and an estimated 50 billion individuals worldwide, are known to be involved in the spread of pathogens, some of which are zoonotic (from animals to humans), such as avian influenza and West Nile viruses. Individual measures of avian health may include physical measurements (e.g., body mass, wing length), pathogen infection status, …
Interactions Between Streptococcus Agalactiae And Candida Albicans Affect Persistence And Virulence, Kathryn Patenaude
Interactions Between Streptococcus Agalactiae And Candida Albicans Affect Persistence And Virulence, Kathryn Patenaude
Electronic Theses and Dissertations
Streptococcus agalactiae (Group B Streptococcus or GBS), a Gram-positive bacterium, and Candida albicans, a polymorphic fungus, are commensal microbes in most of the population they colonize but are also capable of causing severe and sometimes fatal infections in certain patient groups. Both organisms share similarities including the colonization the same tissue environments and causing infections in specific patient groups including those who are newborn, pregnant, suffering from chronic conditions like diabetes and HIV, as well as elderly patients. Previous research discovered that GBS and C. albicans can synergize to enhance the colonization of GBS in the bladders of mice, but …
Pseudomonas Aeruginosa Enhances Fluconazole Activity Against Candida During Polymicrobial Infection, Siham Hattab
Pseudomonas Aeruginosa Enhances Fluconazole Activity Against Candida During Polymicrobial Infection, Siham Hattab
Electronic Theses and Dissertations
Polymicrobial infections pose a significant challenge in the field of medicine due to our incomplete understanding of how pathogens interact during infection and how these interactions impact the effectiveness of drug treatments. Among the opportunistic pathogens, Candida albicans and Pseudomonas aeruginosa stand out, as they are often found in similar infection sites, such as burn wounds, the lungs of patients with cystic fibrosis and those on mechanical ventilation. Candida albicans is an opportunistic fungus responsible for invasive candidiasis, a condition associated with a high mortality rate of 40% in hospitals. Pseudomonas aeruginosa, on the other hand, is the primary pathogen …
Investigating The Impact Of Prophages On Bacterial Fitness Of Streptococcus Agalactiae, Caitlin Wiafe-Kwakye
Investigating The Impact Of Prophages On Bacterial Fitness Of Streptococcus Agalactiae, Caitlin Wiafe-Kwakye
Electronic Theses and Dissertations
Streptococcus agalactiae (Group B Streptococcus or GBS) is a common bacterium found in pregnant women that can cause severe infections in neonates. Although detecting maternal colonization and administering antibiotics during labor can prevent early-onset GBS disease in neonates, antibiotics negatively affect newborns' microbiota, leading to complications like gastrointestinal disorders and immune system dysregulation. Therefore, alternative therapeutic measures are necessary to improve maternal and neonatal outcomes. Understanding GBS disease pathology and developing effective preventive measures and treatments is essential. GBS evolves from a commensal bacterium to an invasive disease-causing pathogen using various mechanisms, such as adapting to the host immune response, …
Weaving An Interdisciplinary Microbiome Career Using Threads From Different Ecosystems, Sarah Hosler
Weaving An Interdisciplinary Microbiome Career Using Threads From Different Ecosystems, Sarah Hosler
Electronic Theses and Dissertations
Animals have trillions of microorganisms living in or on many body sites, these communities of microorganisms are called microbiomes. Microbiomes are typically host-specific, and a lot of information about the host can be determined from investigating them. Microbiome research has many real-world applications, and this thesis utilizes the One Health perspective, which acknowledges the connection of humans, animals, and environments, and emphasizes the need for collaborative, interdisciplinary research. The first interdisciplinary project is an investigation into the bacteria in wild and cultured Atlantic deep-sea scallop, Placopecten magellanicus larvae. Adults in hatcheries can be induced to spawn, but the last two …
Determining Effects Of Management Practices On Potato Early Dying And Soil Microbiome And Assessing Risk Of Fungicide Resistance In Verticillium Dahliae, Kedi Li
Electronic Theses and Dissertations
Potato early dying (PED) is a yield-constraining soilborne disease of potato, caused by Verticillium spp. with V. dahliae being the predominant causal agent. Since the pathogen inhabits soil for long periods, PED management aims to reduce the population of V. dahliae in soil. Benzovindiflupyr and azoxystrobin are effective chemicals and frequently used in the control of V. dahliae. In this study, field trials were conducted at Aroostook Farm, Presque Isle, ME in 2019 and 2020. Chemical and biological products have been studied for PED control, and fungicide resistance was also examined. To evaluate fungicide resistance, benzovindiflupyr was characterized on …
Retroviral Infection Dynamics In Maine's Wild Turkeys, Stephanie A. Shea
Retroviral Infection Dynamics In Maine's Wild Turkeys, Stephanie A. Shea
Electronic Theses and Dissertations
Widespread wild turkey reintroductions in the late 1900s have led to increases in population density and geographic distribution across North America. This rapid population expansion has put them into proximity with closely-related wild and domestic avian species, increasing the risks of pathogen transmission. Lymphoproliferative disease virus (LPDV) is an avian oncogenic retrovirus detected in wild turkeys in 2009, and previously known to infect domestic turkeys. Following its initial detection, surveys reported variable LPDV prevalence across eastern North America with most wild turkeys being asymptomatic, however diagnostic cases revealed 10% mortality of LPDV-infected individuals. Given its recent detection, little is known …
Characterizing The Role Of Prophages On Whib7 Expression And Antibiotic Resistance In Mycobacterium Chelonae, Jaycee J. Cushman
Characterizing The Role Of Prophages On Whib7 Expression And Antibiotic Resistance In Mycobacterium Chelonae, Jaycee J. Cushman
Electronic Theses and Dissertations
Mycobacterial pathogens are responsible for an ongoing public health crisis. Mycobacterium abscessus is the causative agent of lung infections that disproportionately affect immunocompromised individuals and is the most intrinsically antibiotic-resistant bacterial species known. These characteristics make M. abscessus infections difficult to treat, with a success rate of only 45%. While some extensively resistant isolates are caused by mutations in drug targets, others appear to be a result of increased intrinsic drug resistance. Common among these strains is the presence of integrated viral genomes (prophage) that are known to contribute to fitness and antibiotic resistance in other pathogens but whose roles …
Understanding The Adhesion Mechanism In Mycelium-Assisted Wood Bonding, Wenjing Sun
Understanding The Adhesion Mechanism In Mycelium-Assisted Wood Bonding, Wenjing Sun
Electronic Theses and Dissertations
The increasing environmental awareness has led to an increased interest in developing more sustainable materials as alternatives to petroleum-derived products. Among different nature-based products, fungal-mycelium-based bio-composites have gained considerable attention in various applications. Multiple materials with different densities and structures and potential applications can be fabricated by inoculating filamentous white-rot fungi in lignocellulosic materials and other substrates. Different from lower-density as-grown foam-like mycelium composites, higher-density mycelium-lignocellulosic panels have the potential to replace commercial particleboard and fiberboard bonded by petroleum-based resins. This kind of composite can be produced by directly adding heat and pressure to the low-density foams or by assembling …
Deciphering The Perpetual Fight Between Virus And Host: Utilizing Bioinformatics To Elucidate The Host's Genetic Mechanisms That Influence Jc Polyomavirus Infection, Michael P. Wilczek
Deciphering The Perpetual Fight Between Virus And Host: Utilizing Bioinformatics To Elucidate The Host's Genetic Mechanisms That Influence Jc Polyomavirus Infection, Michael P. Wilczek
Electronic Theses and Dissertations
JC polyomavirus (JCPyV) is a human-specific pathogen that infects 50-80% of the population, and can cause a deadly, demyelinating disease, known as progressive multifocal leukoencephalopathy (PML). In most of the population, JCPyV persistently infects the kidneys but during immunosuppression, it can reactivate and spread to the central nervous system (CNS), causing PML. In the CNS, JCPyV targets two cell types, astrocytes, and oligodendrocytes. Due to the hallmark pathology of oligodendrocyte lysis observed in disease, oligodendrocytes were thought to be the main cell type involved during JCPyV infection. However, recent evidence suggests that astrocytes are targeted by the virus and act …
Examining The Function Of Protein Acyltransferase Via The Dhhc Domain Of The Paz5 Protein In The Organism Dictyostelium Discoideum, George M. Stuart-Ranchev
Examining The Function Of Protein Acyltransferase Via The Dhhc Domain Of The Paz5 Protein In The Organism Dictyostelium Discoideum, George M. Stuart-Ranchev
Electronic Theses and Dissertations
Protein S-palmitoylation plays a crucial role in many biological systems. S-palmitoylation involves the post-translational attachment of palmitate to a cysteine residue through a reversible thioester linkage. S-Palmitoylation is used to modify both integral and membrane proteins, many of which are involved in intracellular trafficking, membrane localization, and signal transduction pathways. Intracellular palmitoylation is mediated by a family of protein acyltransferases (PATs). PAT mutations are associated with neurological diseases and cancer progression. Proteins in the PAT family are defined by the presence of a 51-amino acid cysteine-rich domain (CRD), which contains a highly conserved aspartate-histidine-histidine-cysteine (DHHC) motif. The …
Dissection Of Molecular Mechanisms By Which Human Host Factors Regulate Jc Polyomavirus Internalization, Colleen Mayberry
Dissection Of Molecular Mechanisms By Which Human Host Factors Regulate Jc Polyomavirus Internalization, Colleen Mayberry
Electronic Theses and Dissertations
Viruses require a host cell in order to replicate. Infection and the onset of disease result from direct virus-host cell interactions. My dissertation research is focused on understanding how a common human virus, JC polyomavirus (JCPyV), activates specific host cell factors to cause infection. When people are immunocompromised, JCPyV infection may exacerbate into the onset of progressive multifocal leukoencephalopathy (PML), a fatal neurodegenerative disease. Individuals with the greatest risk for the development of PML are those living with multiple sclerosis or infected with HIV. Unfortunately 50-80% of the population is infected by JCPyV, putting individuals at risk for developing PML. …
Does Circularizing Source-Separated Food Waste Present A Risk To Our Food?, Astha Thakali
Does Circularizing Source-Separated Food Waste Present A Risk To Our Food?, Astha Thakali
Electronic Theses and Dissertations
About a third of the food produced annually is wasted. Food waste recycling can be a way to close the loop and attain a more sustainable food system, however, the system must be carefully monitored and managed to avoid the introduction and build-up of contaminants. To study the potential presence of contaminants in food waste, source-separated food waste was collected and screened for five classes of contaminants (physical contaminants, heavy metals, halogenated organic contaminants, pathogens, and antibiotic resistance genes) from two separate regulatory environments (voluntary vs mandated food separation). The regulatory environment did not affect the level of contamination, except …
Quantification Of Interactions Between Influenza Hemagglutinin And Host Cell Phosphoinositides By Super-Resolution Microscopy, Matthew T. Parent
Quantification Of Interactions Between Influenza Hemagglutinin And Host Cell Phosphoinositides By Super-Resolution Microscopy, Matthew T. Parent
Electronic Theses and Dissertations
The influenza viral membrane protein hemagglutinin (HA) forms dense nanoscale clusters on host cell plasma membranes (PM), but the mechanisms that direct HA clustering are not well understood. Previous studies have observed HA associated with actin rich regions of the PM, but there are no known direct interactions between HA and actin. Phosphatidylinositol 4,5-biphosphate (PIP2) is a signaling lipid in the PM which can regulate the actin cytoskeleton, and actin comets initiated by PIP2 are known to be exploited by HA to reach the PM of infected cells. PIP2 is also used by other viruses, such as HIV and Ebola, …
Development Of A High-Throughput Platform For The Determination Of Antiviral Therapeutics, Mason A. Crocker
Development Of A High-Throughput Platform For The Determination Of Antiviral Therapeutics, Mason A. Crocker
Electronic Theses and Dissertations
JC polyomavirus (JCPyV) persists in up to 90% of the global human population. In healthy individuals, the virus resides within the kidneys resulting in a low-level infection. However, in severely immunocompromised individuals, the virus can migrate to the central nervous system (CNS), causing the demyelinating disease progressive multifocal leukoencephalopathy (PML). Currently, this debilitating disease has no clinical therapeutic options and is almost universally fatal. Specifics of the JCPyV infectious cycle, as well as the limitations of traditional laboratory techniques, have previously hindered the search for antiviral agents with the potential to prevent or treat JCPyV infection. To this end, a …
The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga
The Potential For Dickeya Dianthicola To Be Vectored By Two Common Insect Pests Of Potatoes, Jonas K. Insinga
Electronic Theses and Dissertations
Dickeya dianthicola (Samson) causing blackleg and soft rot was first detected in potatoes grown in Maine in 2014. Previous work has suggested that insects, particularly aphids, may be able to vector bacteria in this genus between plants, but no conclusive work has been done to confirm this theory. In order to determine whether insect-mediated transmission is likely to occur in potato fields, two model potato pests common in Maine were used: the Colorado potato beetle (Leptinotarsa decimlineata Say) and the green peach aphids (Myzus persicae Sulzer). Olfactometry and recruitment experiments evaluated if either insect discriminates between infected and …
Intravital Imaging In A Zebrafish Model Elucidates Interactions Between Mucosal Immunity And Pathogenic Fungi, Linda S. Archambault
Intravital Imaging In A Zebrafish Model Elucidates Interactions Between Mucosal Immunity And Pathogenic Fungi, Linda S. Archambault
Electronic Theses and Dissertations
Candida yeasts are common commensals that can cause mucosal disease and life-threatening systemic infections. While many of the components required for defense against Candida albicans infection are well established, questions remain about how various host cells at mucosal sites assess threats and coordinate defenses to prevent normally commensal organisms from becoming pathogenic. Using two Candida species, C. albicans and C. parapsilosis, which differ in their abilities to damage epithelial tissues, we used traditional methods (pathogen CFU, host survival, and host cytokine expression) combined with high-resolution intravital imaging of transparent zebrafish larvae to illuminate host-pathogen interactions at the cellular level …
The Effects Of Seasonal Variations In Chemistry And Hydrology On The Microbial Community And Its Sulfide Oxidation Potential In A Naturally Acidic Maine Stream, Raymond C. Kahler Iii
The Effects Of Seasonal Variations In Chemistry And Hydrology On The Microbial Community And Its Sulfide Oxidation Potential In A Naturally Acidic Maine Stream, Raymond C. Kahler Iii
Electronic Theses and Dissertations
Sulfide minerals oxidize through interaction with water and oxygen, releasing hydrogen ions. The process often occurs naturally near metal sulfide deposits, and can be accelerated through mining. Microorganisms accelerate the rate of sulfide oxidation. Acidified streams typically contain high metal concentrations (e.g. aluminum) and microbes in these systems may develop resistances to metal toxicity. Stream flow can affect sulfide oxidation and microbial community structure. Baseflow can influence stream chemistry from interactions with the surrounding bedrock, while stormflow affects stream chemistry and the local microbial community through dilution and addition of microbes transported by runoff. Microbial community composition is affected by …
Host Mediated Mechanisms Of Fungal Cell Spread In A Transparent Zebrafish Infection Model, Allison Scherer
Host Mediated Mechanisms Of Fungal Cell Spread In A Transparent Zebrafish Infection Model, Allison Scherer
Electronic Theses and Dissertations
Innate immunity has developed elegant processes for the detection and clearance of invasive fungal pathogens. Disseminated candidiasis is of significant concern for those with suppressed immune systems or indwelling medical equipment, and mortality in these groups approaches 70%. Poor patient outcomes have spurred the need to understand how this non-motile pathogen spreads in the host. Technical limitations have previously hindered our ability to visualize the role of innate immunity and host tissue barriers in the spread of C. albicans in vivo. Using the zebrafish model to overcome these limitations, we have examined three potential host-mediated mechanisms of dissemination: movement …
Thermal And Microbial Effects On Brown Macroalgae: Heat Acclimation And The Biodiversity Of The Microbiome, Charlotte Tc Quigley
Thermal And Microbial Effects On Brown Macroalgae: Heat Acclimation And The Biodiversity Of The Microbiome, Charlotte Tc Quigley
Electronic Theses and Dissertations
This dissertation examines effects of stress on brown algal biology from a macroscopic scale by examining the whole aquaculture crops, and at a microscopic level by examining the macroalgal microbiome, across the vertical stress gradient of the intertidal zone and across the latitudes of their biogeographic ranges. Thermal stress negatively affected seedstock gametophytes of the kelp Alaria esculenta isolated from northern and southern locations in Maine. However, previous thermal stress had a positive effect on growth of the next-generation sporophytes. Alaria esculenta has potential as a kelp crop in Maine’s sea vegetable aquaculture sector and implementing this protocol may allow …
Investigating Present-Day Health Issues Of The American Lobster (Homarus Americanus), Deborah A. Bouchard
Investigating Present-Day Health Issues Of The American Lobster (Homarus Americanus), Deborah A. Bouchard
Electronic Theses and Dissertations
The American lobster, Homarus americanus, H. Milne Edwards, 1837, supports the most economically valuable fishery along the North Atlantic coast of North America. A collapse in lobster populations in Southern New England (SNE) has coincided with increasing ocean temperatures and emerging diseases. This research investigated the etiologies of limp lobster disease (LLD) and epizootic shell disease (ESD), two diseases that continue to cause significant mortality in natural lobster populations. Mortality from LLD is associated with the bacteria Photobacterium indicum and is more intense in impounded lobsters. To more clearly define the community ecology of this suspected opportunistic pathogen, the microbial …
Reducing Tillage In Small-Scale Permanent Bed Organic Vegetable Production Systems, Jeremiah D. Vallotton
Reducing Tillage In Small-Scale Permanent Bed Organic Vegetable Production Systems, Jeremiah D. Vallotton
Electronic Theses and Dissertations
The response of field-grown vegetable crops to reduced tillage and mulching in permanent beds was evaluated through measuring crop yields, weed pressure, earthworm counts, and soil basal respiration. Two vegetable crops (“Bush Delicata” squash and “Farao” cabbage) were started in April and May of 2016 and 2017 respectively, transplanted in late June, and harvested on 15-Sep-2016 and 25-Aug-2017. Fruit number and weight of squash, and head weight and feeding damage of cabbage were measured. These results suggest that intensive tillage (8” rototill every year) can be successfully reduced to alternating years of shallow (2”) rototilling and a less intensive form …
Expression Profiling Of Non-Coding Rna By Environmental Interactions In Innate Immunity, Jacob R. Longfellow
Expression Profiling Of Non-Coding Rna By Environmental Interactions In Innate Immunity, Jacob R. Longfellow
Electronic Theses and Dissertations
Cystic fibrosis (CF) is a genetic disorder that affects 30,000 people in the United States and currently has no cure. Although CF affects all of the body’s systems, it is largely characterized as a lung disease. CF is caused by a mutation in both copies of the gene for cystic fibrosis transmembrane conductance regulator (CFTR). A mutation in the CFTR gene leads to improper movement of chloride ions and water into the airways, which dysregulates the airway surface liquid volume and composition. Individuals with CF are prone to lung infections due to inefficient bacterial clearance and by the age of …
Candida And Pseudomonas Interact To Enhance Mucosal Infection In Transparent Zebrafish, Audrey C. Bergeron
Candida And Pseudomonas Interact To Enhance Mucosal Infection In Transparent Zebrafish, Audrey C. Bergeron
Electronic Theses and Dissertations
Polymicrobial communities exist throughout the human body and include both fungi and bacteria. During disease, cross-kingdom interactions among bacteria, fungi, and/or the immune system can alter virulence and lead to complex polymicrobial infections. The fungus C. albicans is among the most commonly isolated fungi in the context of fungal-bacterial co-infections and is often accompanied by the bacterium P. aeruginosa at a variety of sites throughout the body including mucosal tissues such as the lung. In vitro, C. albicans and P. aeruginosa have a cyclic, bi-directional, and largely antagonistic relationship, but these interactions do not account for the role of the …
Investigating High Speed Localization Microscopy Through Experimental Methods, Data Processing Methods, And Applications Of Localization Microscopy To Biological Questions, Andrew J. Nelson
Electronic Theses and Dissertations
Fluorescence Photoactivation Localization Microscopy(FPALM) and other super resolution localization microscopy techniques can resolve structures with nanoscale resolution. Unlike techniques of electron microscopy, they are also compatible with live cell and live animal studies, making FPALM and related techniques ideal for answering questions about the dynamic nature of molecular biology in living systems. Many processes in biology occur on rapid sub second time scales requiring the imaging technique to be capable of resolving these processes not just with a high enough spatial resolution, but with an appropriate temporal resolution. To that end, this Dissertation in part investigates high speed FPALM as …
Dynamic Host-Pathogen Interactions Result In Fungal Epitope Unmasking, Alex Hopke
Dynamic Host-Pathogen Interactions Result In Fungal Epitope Unmasking, Alex Hopke
Electronic Theses and Dissertations
Molecular camouflage is used by a diverse set of pathogens to disguise their identity and avoid recognition by protective host receptors. The opportunistic fungal pathogen Candida albicans is a good example, as it masks the inflammatory component β-glucan in its cell wall to evade detection by the immune receptor Dectin-1. Interestingly, it has been seen that β-glucan becomes unmasked during infection in vivo, though the underlying mechanisms remained unclear. Exposure levels of this epitope may be important, as Dectin-1 mediates protection from some strains of C. albicans and alterations in the organization and composition of the Candida cell wall …
Characterization Of Phycoerythrin Physiology In Low-Light Adapted Prochlorococcus Ecotypes, Kathryn H. Roache-Johnson
Characterization Of Phycoerythrin Physiology In Low-Light Adapted Prochlorococcus Ecotypes, Kathryn H. Roache-Johnson
Electronic Theses and Dissertations
The marine cyanobacteria Prochlorococcus and Synechococcus are the most abundant phototrophs in the oceans. They cohabit the oligotrophic ocean and thus have coevolved together, yet they have distinctly different methods for harvesting light. Synechococcus, like other cyanobacteria, possess phycobilisomes with various combinations of phycobiliproteins to capture wavelengths of light not otherwise available to chlorophyll. Prochlorococcus lack phycobilisomes and use divinyl chlorophyll b (Chl b2) as their primary accessory pigment to divinyl chlorophyll a (Chl a2) to capture light energy. In addition to the divinyl chlorophylls, Prochlorococcus has genes associated with the phycobiliprotein phycoerythrin (PE), the role of which is still …
Distribution And Diversity Of Bacterial Chemolithotrophs In Marine And Freshwater Sediments, Lisa M. Nigro
Distribution And Diversity Of Bacterial Chemolithotrophs In Marine And Freshwater Sediments, Lisa M. Nigro
Electronic Theses and Dissertations
Bacterial chemolithotroph population structure has been investigated in Lowes Cove marine intertidal mudflat and Damariscotta Lake, Maine. A 492 to 495 fragment of the cbbL gene, coding for the large subunit of Form I ribulose-1,5- bisphosphate carboxylase/oxygenase (rubisco) was amplified from lake surface (upper 2 mm) sediments and mudflat surface (upper 2 mm), subsurface (5-7 cm), and Mya arenaria burrow wall sediments, as well as sulfide-oxidizing bacterial mat samples. Amplified DNA was used to construct cbbL clone libraries. Phylogenetic analysis showed that Damariscotta Lake cbbL clones were mainly of the 1C type, indicating a facultative carbon monoxide/hydrogen-oxidizing community. Conversely, clones …
Molecular Padlock Assay Of Crude Plant Leaf Extracts For Detection Of Listeria Monocytogenes, Allison L. Hurlburt
Molecular Padlock Assay Of Crude Plant Leaf Extracts For Detection Of Listeria Monocytogenes, Allison L. Hurlburt
Electronic Theses and Dissertations
A molecular padlock assay was developed and assessed for detection of Listeria monocytogenes that operated in crude plant extracts. The molecular padlock assay was developed by Liu et al. (1996) and modified by Lizardi et al. (1998). We further modified and described a padlock probe that detected L. monocytogenes oligonucleotide, cDNA and genomic DNA containing a 16s rRNA sequence (GenBank Acc. No. X56 153). This technique was effective in the presence of crude potato leaf extracts in contrast to PCR, which failed to detect the presence of L. monocytogenes targets in crude leaf extracts. Sensitivity of the padlock procedure was …
Molecular Characterization Of Zebrafish Interferon, Mx, And Mx Promoter, Stephen Altmann
Molecular Characterization Of Zebrafish Interferon, Mx, And Mx Promoter, Stephen Altmann
Electronic Theses and Dissertations
Type I interferons (IFNs) represent a family of biological molecules whose antiviral, antitumor, and immunomodulatory role is well known. IFNs were first identified in the 1950's and have since been used extensively for the treatment of various cancers, and viral infections. In order to more fully characterize the IFN response, it is often necessary to use animal models. Although the mouse has been used extensively for IFN studies, a lower order vertebrate model is also desirable, as it would provide information about the structure and function of a more ancestral IFN. To this end, herein is described the cloning and …