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Finalizing The Genome Annotation Of Model Cluster E Mycobacteriophage Ukulele Via Rna-Seq Analysis, Jackson R. Foley
Finalizing The Genome Annotation Of Model Cluster E Mycobacteriophage Ukulele Via Rna-Seq Analysis, Jackson R. Foley
Honors College
Mycobacteriophage (phage) are viruses that infect bacteria of the genus Mycobacterium, including pathogenic M. tuberculosis and non-pathogenic M. smegmatis. Temperate phages are capable of undergoing both lytic and lysogenic infection. In lytic infections, phage lyse the host cell after replication. In lysogenic infection, the phage integrates its genome into the host genome (prophage) and replicates with the host. All pathogenic strains of Mycobacterium carry prophage that potentially contribute to host virulence and fitness. Formation and maintenance of these prophage is not well understood, particularly for cluster E phage. This project characterizes gene product (gp) 53, a potential Cro-like …
Characterization Of Transcriptional Control Elements In Cluster E Mycobacteriophage Ukulele, Campbell Belisle Haley
Characterization Of Transcriptional Control Elements In Cluster E Mycobacteriophage Ukulele, Campbell Belisle Haley
Honors College
Mycobacteriophage (phage) are a diverse group of viruses that infect Mycobacterium. Their study allows further understanding of viral evolution and genetics. Phage tightly control gene expression and transcribe their genes using host RNA polymerases. This project identifies potential transcriptional control elements in the genome of mycobacteriophage Ukulele. Promoters are sequences of the genome that allow binding of RNA polymerase and initiation of transcription. 21 putative promoters were identified in the Ukulele genome. To confirm transcriptional activity from putative promoters, a GFP reporter system was developed in mycobacterial cells. Intrinsic terminators are mRNA sequences that form secondary structure during transcription …
Complete Annotations Of The Cluster E Mycobacteriophage Ukulele Genome And Characterization Of Cluster E Lysogeny Regulation, Gwendolyn M. Beacham
Complete Annotations Of The Cluster E Mycobacteriophage Ukulele Genome And Characterization Of Cluster E Lysogeny Regulation, Gwendolyn M. Beacham
Honors College
Mycobacteriophages (phages) are diverse and abundant viruses that infect species of the genus Mycobacterium. Mycobacteriophages are categorized into clusters based primarily on nucleotide similarity (18). Some clusters are well-characterized, while others, such as Cluster E, are poorly characterized (20). There are 54 members of Cluster E (39) including the phage Ukulele that was isolated at the University of Maine in 2011. This thesis is aimed towards characterizing Cluster E phages using Ukulele as a model. Cluster E phages have long tails of approximately 300 nm and they produce slightly turbid plaques on a lawn of Mycobacterium smegmatis. Putative …