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Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Leptin Effects On Bone Metabolism, Elizabeth Bark
Leptin Effects On Bone Metabolism, Elizabeth Bark
Williams Honors College, Honors Research Projects
Scales from adult zebrafish, Danio rerio, are an established model for studying osteoblast and osteoclast activity. In this study, scales in a cell culture were used to determine if the hormone leptin has an effect on activity of these bone remodeling scales. Alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) activities of zebrafish scales were measured and compared with and without leptin treatment. There was no statistically significant difference between leptin treatments in terms of ALP and TRAP activity. Differences between TRAP values between days 1 and 2 did look promising although they were not statistically significant. This study …
Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara
Non-Invasive Method For Leptin Supplementation In Zebrafish (Danio Rerio), Regan Mcnamara
Williams Honors College, Honors Research Projects
I tested the hypothesis that recombinant leptin protein can be introduced to zebrafish in vivo through non-invasive soaking in a solution containing the protein. One way to study various molecules’ effects in vivo is through intraperitoneal or intracerebroventricular injections during the embryonic or larval stage, which is invasive, difficult to administer, and can have a high mortality rate. 48 hours post fertilization (hpf) zebrafish were soaked in a His-tagged recombinant leptin protein solution at 10 nM and 100 nM concentrations (produced by Genscript). After soaking, zebrafish larvae were washed extensively to remove all recombinant protein on their exterior before homogenization. …
Preliminary Investigations Into Endospanin Expression In Zebrafish, Brittany Gray
Preliminary Investigations Into Endospanin Expression In Zebrafish, Brittany Gray
Williams Honors College, Honors Research Projects
This study investigated endospanin expression in zebrafish by performing western blots to identify which tissues expressed endospanin. A feeding study was also conducted to see if endospanin expression varied in fed versus unfed fish. After tissues were dissected, protein assays were performed on homogenized tissues and then gels were run using gel electrophoresis. The primary antibody rabbit anti-endospanin peptide was used on the finished blots. Chemiluminescence was then used to visualize the blots using GeneSnap software. Endospanin expression was evident in heart, liver, and ovary tissues. The feeding study showed that endospanin expression in liver tissue was not significantly different …