Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Differential Intrasplenic Migration Of Dendritic Cell Subsets Tailors Adaptive Immunity., Samuele Calabro, Dong Liu, Antonia Gallman, Manuela Sales L Nascimento, Zizi Yu, Ting-Ting Zhang, Pei Chen, Biyan Zhang, Lan Xu, Uthaman Gowthaman, Jayendra Kumar Krishnaswamy, Ann M Haberman, Adam Williams, Stephanie C Eisenbarth
Differential Intrasplenic Migration Of Dendritic Cell Subsets Tailors Adaptive Immunity., Samuele Calabro, Dong Liu, Antonia Gallman, Manuela Sales L Nascimento, Zizi Yu, Ting-Ting Zhang, Pei Chen, Biyan Zhang, Lan Xu, Uthaman Gowthaman, Jayendra Kumar Krishnaswamy, Ann M Haberman, Adam Williams, Stephanie C Eisenbarth
Faculty Research 2016
Evidence suggests that distinct splenic dendritic cell (DC) subsets activate either CD4+ or CD8+ T cells in vivo. This bias has been partially ascribed to differential antigen presentation; however, all DC subsets can activate both T cell lineages in vitro. Therefore, we tested whether the organization of DC and T cell subsets in the spleen dictated this preference. We discovered that CD4+ and CD8+ T cells segregated within splenic T cell zones prior to immunization. After intravenous immunization, the two major conventional DC populations, distinguished by 33D1 and XCR1 staining, migrated into separate regions of the T cell zone: 33D1+ …
Towards A Standard Ontology Metadata Model., Hua Min, Stuart Turner, Sherri De Coronado, Brian Davis, Trish Whetzel, Robert R. Freimuth, Harold R. Solbrig, Richard Kiefer, Grace Stafford, Lawrence Wright, Riki Ohira
Towards A Standard Ontology Metadata Model., Hua Min, Stuart Turner, Sherri De Coronado, Brian Davis, Trish Whetzel, Robert R. Freimuth, Harold R. Solbrig, Richard Kiefer, Grace Stafford, Lawrence Wright, Riki Ohira
Faculty Research 2016
No abstract provided.
Improved In Vitro Fertilization Via Zona Thinning., Jax Mice Clinical And Research Services
Improved In Vitro Fertilization Via Zona Thinning., Jax Mice Clinical And Research Services
Faculty Research 2016
Mouse in vitro fertilization (IVF) can be used to rapidly expand mouse lines from a few males that carry the desired genotype or to maintain strains with poor breeding efficiency. This guide describes a detailed method using acidified Tyrode solution to open the zona pellucida, and increase the fertilization capacity of frozen mouse sperm. The embryos generated are suitable for transfer into pseudopregnant recipent females, culture or cryopreseration.