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Full-Text Articles in Life Sciences
Transcriptional Response Of Mexican Axolotls To Ambystoma Tigrinum Virus (Atv) Infection, J. D. Cotter, A. Storfer, Robert B. Page, C. K. Beachy, S. R. Voss
Transcriptional Response Of Mexican Axolotls To Ambystoma Tigrinum Virus (Atv) Infection, J. D. Cotter, A. Storfer, Robert B. Page, C. K. Beachy, S. R. Voss
Biology Faculty Publications
Background: Very little is known about the immunological responses of amphibians to pathogens that are causing global population declines. We used a custom microarray gene chip to characterize gene expression responses of axolotls (Ambystoma mexicanum) to an emerging viral pathogen, Ambystoma tigrinum virus (ATV). Result: At 0, 24, 72, and 144 hours post-infection, spleen and lung samples were removed for estimation of host mRNA abundance and viral load. A total of 158 up-regulated and 105 down-regulated genes were identified across all time points using statistical and fold level criteria. The presumptive functions of these genes suggest a robust innate immune …
Effect Of Thyroid Hormone Concentration On The Transcriptional Response Underlying Induced Metamorphosis In The Mexican Axolotl (Ambystoma), Robert B. Page, S. R. Voss, A. K. Samuels, J. J. Smith, S. Putta, C. K. Beachy
Effect Of Thyroid Hormone Concentration On The Transcriptional Response Underlying Induced Metamorphosis In The Mexican Axolotl (Ambystoma), Robert B. Page, S. R. Voss, A. K. Samuels, J. J. Smith, S. Putta, C. K. Beachy
Biology Faculty Publications
Background: Thyroid hormones (TH) induce gene expression programs that orchestrate amphibian metamorphosis. In contrast to anurans, many salamanders do not undergo metamorphosis in nature. However, they can be induced to undergo metamorphosis via exposure to thyroxine (T4). We induced metamorphosis in juvenile Mexican axolotls (Ambystoma mexicanum) using 5 and 50 nM T4, collected epidermal tissue from the head at four time points (Days 0, 2, 12, 28), and used microarray analysis to quantify mRNA abundances. Results: Individuals reared in the higher T4 concentration initiated morphological and transcriptional changes earlier and completed metamorphosis by Day 28. In contrast, initiation of metamorphosis …
Rapid Dissemination Of Francisella Tularensis And The Effect Of Route Of Infection, S. S. Ojeda, Z. J. Wang, Chris A. Mares, T. A. Chang, Q. Li, E. G. Morris, P. A. Jerabek, J. M. Teale
Rapid Dissemination Of Francisella Tularensis And The Effect Of Route Of Infection, S. S. Ojeda, Z. J. Wang, Chris A. Mares, T. A. Chang, Q. Li, E. G. Morris, P. A. Jerabek, J. M. Teale
Biology Faculty Publications
Background. Francisella tularensis subsp. tularensis is classified as a Category A bioweapon that is capable of establishing a lethal infection in humans upon inhalation of very few organisms. However, the virulence mechanisms of this organism are not well characterized. Francisella tularensis subsp. novicida, which is an equally virulent subspecies in mice, was used in concert with a microPET scanner to better understand its temporal dissemination in vivo upon intranasal infection and how such dissemination compares with other routes of infection. Adult mice were inoculated intranasally with F. tularensis subsp. novicida radiolabeled with 64Cu and imaged by microPET at 0.25, 2 …