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Development Of Pcr-Based Detection System For Soft Rot Pectobacteriaceae Pathogens Using Molecular Signatures, Md Niamul Kabir, Ali Taheri, C. Korsi Dumenyo
Development Of Pcr-Based Detection System For Soft Rot Pectobacteriaceae Pathogens Using Molecular Signatures, Md Niamul Kabir, Ali Taheri, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
Pectobacterium and Dickeya species, usually referred to as soft rot Enterobacteriaceae, are phytopathogenic genera of bacteria that cause soft rot and blackleg diseases and are responsible for significant yield losses in many crops across the globe. Diagnosis of soft rot disease is difficult through visual disease symptoms. Pathogen detection and identification methods based on cultural and morphological identification are time-consuming and not always reliable. A polymerase chain reaction (PCR)-based detection method with the species-specific primers is fast and reliable for detecting soft rot pathogens. We have developed a specific and sensitive detection system for some species of soft rot Pectobacteriaceae …
The Bacterial Soft Rot Pathogens, Pectobacterium Carotovorum And P. Atrosepticum, Respond To Different Classes Of Virulence-Inducing Host Chemical Signals, Paul A. Agyemang, Md Niamul Kabir, Caleb M. Kersey, C. Korsi Dumenyo
The Bacterial Soft Rot Pathogens, Pectobacterium Carotovorum And P. Atrosepticum, Respond To Different Classes Of Virulence-Inducing Host Chemical Signals, Paul A. Agyemang, Md Niamul Kabir, Caleb M. Kersey, C. Korsi Dumenyo
Agricultural and Environmental Sciences Faculty Research
Soft rot bacteria of the Pectobacterium and Dickeya genera are Gram-negative phytopathogens that produce and secrete plant cell wall-degrading enzymes (PCWDE), the actions of which lead to rotting and decay of their hosts in the field and in storage. Host chemical signals are among the factors that induce the bacteria into extracellular enzyme production and virulence. A class of compounds (Class I) made up of intermediate products of cell wall (pectin) degradation induce exoenzyme synthesis through KdgR, a global negative regulator of exoenzyme production. While the KdgR− mutant of P. carotovorum is no longer inducible by Class I inducers, we …