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Full-Text Articles in Life Sciences
Uneven Recombination Rate And Linkage Disequilibrium Across A Reference Snp Map For Common Bean (Phaseolus Vulgaris L.), Matthew W. Blair, Andrés J. Cortés, Andrew D. Farmer, Wei Huang, Daniel Ambachew, R. Varma Penmetsa, Noelia Carrasquilla-Garcia, Teshale Assefa, Steven B. Cannon
Uneven Recombination Rate And Linkage Disequilibrium Across A Reference Snp Map For Common Bean (Phaseolus Vulgaris L.), Matthew W. Blair, Andrés J. Cortés, Andrew D. Farmer, Wei Huang, Daniel Ambachew, R. Varma Penmetsa, Noelia Carrasquilla-Garcia, Teshale Assefa, Steven B. Cannon
Agricultural and Environmental Sciences Faculty Research
Recombination (R) rate and linkage disequilibrium (LD) analyses are the basis for plant breeding. These vary by breeding system, by generation of inbreeding or outcrossing and by region in the chromosome. Common bean (Phaseolus vulgaris L.) is a favored food legume with a small sequenced genome (514 Mb) and n = 11 chromosomes. The goal of this study was to describe R and LD in the common bean genome using a 768-marker array of single nucleotide polymorphisms (SNP) based on Trans-legume Orthologous Group (TOG) genes along with an advanced-generation Recombinant Inbred Line reference mapping population (BAT93 x Jalo EEP558) …
Weed Dynamics During Transition To Conservation Agriculture In Western Kenya Maize Production, Judith A. Odhiambo, Urszula Norton, Dennis Ashilenje, Emmanuel C. Omondi, Jay B. Norton
Weed Dynamics During Transition To Conservation Agriculture In Western Kenya Maize Production, Judith A. Odhiambo, Urszula Norton, Dennis Ashilenje, Emmanuel C. Omondi, Jay B. Norton
Agricultural and Environmental Sciences Faculty Research
Weed competition is a significant problem in maize (Zea mays, L.) production in Sub-Saharan Africa. Better understanding of weed management and costs in maize intercropped with beans (Phaseolus vulgaris, L.) during transition to conservation agricultural systems is needed. Changes in weed population and maize growth were assessed for a period of three years at Bungoma where crops are grown twice per year and at Trans-Nzoia where crops are grown once per year. Treatments included three tillage practices: minimum (MT), no-till (NT) and conventional (CT) applied to three cropping systems: continuous maize/bean intercropping (TYPICAL), maize/bean intercropping with relayed mucuna after bean …
Differentially Expressed Genes In Resistant And Susceptible Common Bean (Phaseolus Vulgaris L.) Genotypes In Response To Fusarium Oxysporum F. Sp. Phaseoli, Renfeng Xue, Jing Wu, Zhendong Zhu, Lanfen Wang, Xiaoming Wang, Shumin Wang, Matthew W. Blair
Differentially Expressed Genes In Resistant And Susceptible Common Bean (Phaseolus Vulgaris L.) Genotypes In Response To Fusarium Oxysporum F. Sp. Phaseoli, Renfeng Xue, Jing Wu, Zhendong Zhu, Lanfen Wang, Xiaoming Wang, Shumin Wang, Matthew W. Blair
Agricultural and Environmental Sciences Faculty Research
Fusarium wilt of common bean (Phaseolus vulgaris L.), caused by Fusarium oxysporum Schlechtend.:Fr. f.sp. phaseoli (Fop), is one of the most important diseases of common beans worldwide. Few natural sources of resistance to Fop exist and provide only moderate or partial levels of protection. Despite the economic importance of the disease across multiple crops, only a few of Fop induced genes have been analyzed in legumes. Therefore, our goal was to identify transcriptionally regulated genes during an incompatible interaction between common bean and the Fop pathogen using the cDNA amplified fragment length polymorphism (cDNA-AFLP) technique. We generated a …
Transcriptome Sequencing Of Mung Bean (Vigna Radiate L.) Genes And The Identification Of Est-Ssr Markers, Honglin Chen, Lixia Wang, Suhua Wang, Chunji Liu, Matthew Wohlgemuth Blair, Xuzhen Cheng
Transcriptome Sequencing Of Mung Bean (Vigna Radiate L.) Genes And The Identification Of Est-Ssr Markers, Honglin Chen, Lixia Wang, Suhua Wang, Chunji Liu, Matthew Wohlgemuth Blair, Xuzhen Cheng
Agricultural and Environmental Sciences Faculty Research
Mung bean (Vigna radiate (L.) Wilczek) is an important traditional food legume crop, with high economic and nutritional value. It is widely grown in China and other Asian countries. Despite its importance, genomic information is currently unavailable for this crop plant species or some of its close relatives in the Vigna genus. In this study, more than 103 million high quality cDNA sequence reads were obtained from mung bean using Illumina paired-end sequencing technology. The processed reads were assembled into 48,693 unigenes with an average length of 874 bp. Of these unigenes, 25,820 (53.0%) and 23,235 (47.7%) showed significant …
Interplanting Annual Ryegrass, Wheat, Oat, And Corn To Mitigate Iron Deficiency In Dry Beans, Emmanuel Chiwo Omondi, Andrew R. Kniss
Interplanting Annual Ryegrass, Wheat, Oat, And Corn To Mitigate Iron Deficiency In Dry Beans, Emmanuel Chiwo Omondi, Andrew R. Kniss
Agricultural and Environmental Sciences Faculty Research
This study evaluated whether grass intercropping can be used to alleviate Fe deficiency chlorosis in dry beans (Phaseolus vulgaris L.) grown in high pH, calcareous soils with low organic matter. Field studies were conducted at the University of Wyoming Sustainable Agriculture Research and Extension Center in 2009 and 2010. Black- and navy beans were grown alone or intercropped with annual ryegrass (Lolium multiflorum Lam.), oat (Avena sativa L.), corn (Zea mays L.), or spring wheat (Triticum aestivum L.) in a two-factor factorial strip-plot randomized complete block design. All four grass species increased chlorophyll intensity in dry beans. However, grass species …
Bac-End Microsatellites From Intra And Inter-Genic Regions Of The Common Bean Genome And Their Correlation With Cytogenetic Features, Matthew Wohlgemuth Blair, Juana Marcela Córdoba, Claritza Muñóz, Deissy K. Yuyó
Bac-End Microsatellites From Intra And Inter-Genic Regions Of The Common Bean Genome And Their Correlation With Cytogenetic Features, Matthew Wohlgemuth Blair, Juana Marcela Córdoba, Claritza Muñóz, Deissy K. Yuyó
Agricultural and Environmental Sciences Faculty Research
Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.). These so called “singleton” BACs were from the G19833 Andean gene pool physical map and the new BES-SSR markers were used for the saturation of the inter-gene pool, DOR364×G19833 genetic map. A total of 899 SSR loci were found among the singleton BES, but only 346 loci corresponded to the single di- or tri-nucleotide motifs that were likely …