Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 5 of 5
Full-Text Articles in Life Sciences
Vibrational Spectroscopy For In Vitro Monitoring Stem Cell Differentiation, Francesca Ravera, Esen Efeoglu, Hugh Byrne
Vibrational Spectroscopy For In Vitro Monitoring Stem Cell Differentiation, Francesca Ravera, Esen Efeoglu, Hugh Byrne
Other resources
Stem cell technology has attracted considerable attention over recent decades due to its enormous potential in regenerative medicine and disease therapeutics. Studying the underlying mechanisms of stem cell differentiation and tissue generation is critical, and robust methodologies and different technologies are required. Towards establishing improved understanding and optimised triggering and control of differentiation processes, analytical techniques such as flow cytometry, immunohistochemistry, reverse transcription polymerase chain reaction, RNA in situ hybridisation analysis, and fluorescence-activated cell sorting have contributed much. However, progress in the field remains limited because such techniques provide only limited information, as they are only able to address specific, …
Data Mining Raman Microspectroscopic Responses Of Cells To Drugs In Vitro Using Multivariate Curve Resolution-Alternating Least Squares, David Perezguaita, Guillermo Quintas, Zeineb Farhane, Roma Tauler, Hugh Byrne
Data Mining Raman Microspectroscopic Responses Of Cells To Drugs In Vitro Using Multivariate Curve Resolution-Alternating Least Squares, David Perezguaita, Guillermo Quintas, Zeineb Farhane, Roma Tauler, Hugh Byrne
Articles
Raman microspectroscopy is gaining popularity for the analysis of time-dependent biological processes such as drug uptake and cellular response. It is a label-free technique which acquires signals from a large variety of components, including cell biomolecules and exogenous compounds such as drugs and nanoparticles, and is commonly employed for in vitro analysis of cells and cell populations with no labelling or staining required. By monitoring the changes to the Raman spectra of the cell as a result of a perturbing agent (e.g. exposure to a drug or toxic agent), one can study the associated changes in cell biochemistry involved in …
Potential Of Raman Spectroscopy For The Analysis Of Plasma/Serum In The Liquid State: Recent Advances, Drishya Rajan Parachalil, Jennifer Mcintyre, Hugh Byrne
Potential Of Raman Spectroscopy For The Analysis Of Plasma/Serum In The Liquid State: Recent Advances, Drishya Rajan Parachalil, Jennifer Mcintyre, Hugh Byrne
Articles
There is compelling evidence in the literature to support the application of Raman spectroscopy for analysis of bodily fluids in their native liquid state. Naturally, the strategies described in the literature for Raman spectroscopic analysis of liquid samples have advantages and disadvantages. Herein, recent advances in the analysis of plasma/serum in the liquid state are reviewed. The potential advantages of Raman analysis in the liquid form over the commonly employed infrared absorption analysis in the dried droplet form are initially highlighted. Improvements in measurement protocols based on inverted microscopic geometries, clinically adaptable substrates, data preprocessing and analysis, and applications for …
Development Of Methodologies For Raman Spectral Analysis Of Human Saliva For Detection Of Oral Cancer, Genecy Calado
Development Of Methodologies For Raman Spectral Analysis Of Human Saliva For Detection Of Oral Cancer, Genecy Calado
Doctoral
Oral cancer is one of the most common malignancies worldwide, with over 350,000 to 400,000 new cases reported each year. Early detection, followed by appropriate treatment, can increase cure rates to 80 or 90%, and greatly improve the quality of life by minimising extensive, debilitating treatments. Usually, the clinical diagnosis of most head and neck neoplasms, including oral cancer, is performed through time-consuming and invasive biopsies followed by histological examination of the excised tissue and may present psychological trauma and risk of infection to patients. In addition, histological grading can be subjective, as it is based on subtle morphological changes. …
Prediction Of Warner-Bratzler Shear Force, Intramuscular Fat, Drip-Loss And Cook-Loss In Beef Via Raman Spectroscopy And Chemometrics, Raquel Cama-Moncunill, Jamie Cafferky, Caroline Augier, Torres Sweeney, Paul Allen, Alessandro Ferragina, Carl Sullivan, Andrew Cromie, Ruth Hamill
Prediction Of Warner-Bratzler Shear Force, Intramuscular Fat, Drip-Loss And Cook-Loss In Beef Via Raman Spectroscopy And Chemometrics, Raquel Cama-Moncunill, Jamie Cafferky, Caroline Augier, Torres Sweeney, Paul Allen, Alessandro Ferragina, Carl Sullivan, Andrew Cromie, Ruth Hamill
Articles
Rapid prediction of beef quality remains a challenge for meat processors. This study evaluated the potential of Raman spectroscopy followed by chemometrics for prediction of Warner-Bratzler shear force (WBSF), intramuscular fat (IMF), ultimate pH, drip-loss and cook-loss. PLS regression models were developed based on spectra recorded on frozen-thawed day 2 longissimus thoracis et lumborum muscle and validated using test sets randomly selected 3 times. With the exception of ultimate pH, models presented notable performance in calibration (R2 ranging from 0.5 to 0.9; low RMSEC) and, despite variability in the results, promising predictive ability: WBSF (RMSEP ranging from 4.6 to 9 …