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Full-Text Articles in Life Sciences
Identification And Validation Of Microsatellite Markers In Strawberry Tree(Arbutus Unedo L.), Pedro Fazenda, Ricardo Pereira, Maria Fonseca, Jorge Carlier, José Leitão
Identification And Validation Of Microsatellite Markers In Strawberry Tree(Arbutus Unedo L.), Pedro Fazenda, Ricardo Pereira, Maria Fonseca, Jorge Carlier, José Leitão
Turkish Journal of Agriculture and Forestry
Strawberry tree (Arbutus unedo L.), an evergreen shrub/small tree of the family Ericaceae, is a main constituent of the Mediterranean basin flora; although it is also found in southwestern France, Macaronesia, and Ireland. The small fruits are edible but mostly used for preparation of preserves and jams, and for liquors such as the Portuguese traditional "aguardente de medronho". Traditionally cultivated by small farmers, often in consociation with Quercus sp., strawberry tree is presently emerging as a new important fruit crop cultivated in large orchards by modern export-oriented enterprises. This change of paradigm requires a growing role of plant breeding, upstream …
Diversity Of Avocado (Persea Americana Mill.) Cultivars From Antioquia (Northeastcolombia) And Comparison With A Worldwide Germplasm Collection, Gloria Patricia Cañas-Gutiérrez, Librada Alcaraz, Jose Ignacio Hormaza, Rafael Eduardo Arango- Isaza, Clara Inés Saldamando-Benjumea
Diversity Of Avocado (Persea Americana Mill.) Cultivars From Antioquia (Northeastcolombia) And Comparison With A Worldwide Germplasm Collection, Gloria Patricia Cañas-Gutiérrez, Librada Alcaraz, Jose Ignacio Hormaza, Rafael Eduardo Arango- Isaza, Clara Inés Saldamando-Benjumea
Turkish Journal of Agriculture and Forestry
In this study, the genetic diversity of 90 avocado (Persea americana Mill) cultivars from Antioquia (Colombia) was compared to 67 germplasm collection accessions using 14 microsatellites. An average of 4.32 ± 2.0 alleles per locus was found, as observed in previous studies. The expected and observed heterozygosity ranges were 0.384-0.724 and 0.393-0.686, respectively. The Antioquian avocados were genetically structured according to an analysis of molecular variance test (fixation index (FST) = 0.054, P < 0.00001). An unweighted pair group method with arithmetic mean (UPGMA) dendrogram with FST paired data produced 2 clusters: one composed by Antioquian avocados and the other by the germplasm collection. Another UPGMA dendrogram with individual Nei-Li distances and additional STRUCTURE analysis separated the Antioquian avocados into 3 clusters (K = 3). Combining samples from the Colombian and germplasm collections produced K = 2. Genetic differentiations between the Antioquian and worldwide avocado germplasm collection could be due to crosses within the Antioquian avocados having been enhanced by insect pollinators, whereas avocados stored in the germplasm collection were constituted by cultivars with known genetic origins. Findings from this study demonstrated that criollo avocado genetics are unique in Antioquia, since the species has been naturally crossed in the field and its closest accession is from Guatemala. Nevertheless, it is important to continue genotyping this species in other locations in Colombia from Sylvester and cultivar populations of this crop to determine its origin.
Molecular Characterization Of Apple (Malus × Domestica Borkh.) Genotypes Originatingfrom Three Complementary Conservation Strategies, Anca Butiuc, Ana Coste, Anca Farkas, Victoria Cristea, Valentina Isac, Adela Halmagyi
Molecular Characterization Of Apple (Malus × Domestica Borkh.) Genotypes Originatingfrom Three Complementary Conservation Strategies, Anca Butiuc, Ana Coste, Anca Farkas, Victoria Cristea, Valentina Isac, Adela Halmagyi
Turkish Journal of Agriculture and Forestry
Apple (Malus × domestica Borkh.) genotypes originating from different plant collections (field collection, in vitro plant collections undergoing or not undergoing cryopreservation) were screened and characterized by SSR markers. Shoot tips excised from plants grown in vitro were successfully cryopreserved by encapsulation-dehydration. The highest regrowth frequency (69%, cultivar Goldrush) of cryopreserved apices was achieved after 24 h of osmoprotection in 0.5 M sucrose, 3 h of desiccation, and 24% water content of alginate beads. No differences in morphological characteristics including shoot length and number and length of roots were observed between controls and plants recovered after cryopreservation. SSR markers were …
Characterizing Microsatellite Polymorphisms Using Assembly-Based Andmapping-Based Tools, Gülfem Demi̇r, Can Alkan
Characterizing Microsatellite Polymorphisms Using Assembly-Based Andmapping-Based Tools, Gülfem Demi̇r, Can Alkan
Turkish Journal of Biology
Microsatellite polymorphism has always been a challenge for genome assembly and sequence alignment due to sequencing errors, short read lengths, and high incidence of polymerase slippage in microsatellite regions. Despite the information they carry being very valuable, microsatellite variations have not gained enough attention to be a routine step in genome sequence analysis pipelines. After the completion of the 1000 Genomes Project, which aimed to establish the most detailed genetic variation catalog for humans, the consortium released only two microsatellite prediction sets generated by two tools. Many other large research efforts have failed to shed light on microsatellite variations. We …