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Effects Of Notch Signalling On The Expression Of Sema3c, Hmga2, Cxcl14, Cxcr7, And Ccl20 In Breast Cancer, Cansu Küçükköse, Özden Yalçin Özuysal
Effects Of Notch Signalling On The Expression Of Sema3c, Hmga2, Cxcl14, Cxcr7, And Ccl20 In Breast Cancer, Cansu Küçükköse, Özden Yalçin Özuysal
Turkish Journal of Biology
Metastasis is the main reason for death in breast cancer. Understanding the molecular players in metastasis is crucial for diagnostic and therapeutic purposes. Notch signalling plays an oncogenic role in breast tumorigenesis and is involved in metastasis. Downstream mediators of Notch signalling in prometastatic processes are not yet fully discovered. Here we aimed to investigate whether Notch signalling regulates the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20, which are involved in prometastatic processes, in breast cell lines. To this end, expression of the selected genes was analysed following Notch activation by overexpression of the Notch1 intracellular domain in the …
E2f6 Is Essential For Cell Viability In Breast Cancer Cells During Replication Stress, Inam Lafta
E2f6 Is Essential For Cell Viability In Breast Cancer Cells During Replication Stress, Inam Lafta
Turkish Journal of Biology
E2F6 is a member of the E2F family of transcription factors involved in regulation of a wide variety of genes through both activation and repression. E2F6 has been reported as overexpressed in breast cancers but whether or not this is important for tumor development is unclear. We first checked E2F6 expression in tumor cDNAs and the protein level in a range of breast cancer cell lines. RNA interference-mediated depletion was then used to assess the importance of E2F6 expression in cell lines with regard to cell cycle profile using fluorescence-activated cell sorting and a cell survival assay using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide …
Response Of Breast Cancer Cells To Ifnî±-2b In 2d And 3d Cell Cultures, Tetiana Herheliuk, Olena Perepelytsina, Andrij Ugnivenko, Lyudmila Ostapchenko, Mychailo Sydorenko
Response Of Breast Cancer Cells To Ifnî±-2b In 2d And 3d Cell Cultures, Tetiana Herheliuk, Olena Perepelytsina, Andrij Ugnivenko, Lyudmila Ostapchenko, Mychailo Sydorenko
Turkish Journal of Biology
The effect of IFNα-2b on the migration, proliferation, and expression of epithelial and mesenchymal markers of MCF-7 tumor adenocarcinoma cells in 2D and 3D cell cultures was examined. A significant cytostatic effect of IFNα-2b on the tumor population was detected. It was found that changes in the expression of epithelial (CKs and EpCAM) and mesenchymal markers were caused by changing the growth type of the tumor population. IFNα-2b inhibited migration of tumor cells to the suspension fraction and promoted an increase in expression of CK and EpCAM in 2D and 3D cell cultures, but only in the 3D culture was …