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Full-Text Articles in Life Sciences
Oocyte Development In Melanogryllus Desertus (Pallas, 1771) (Orthoptera: Gryllidae):Presence Of Balbiani Body, Özlem Çakici
Oocyte Development In Melanogryllus Desertus (Pallas, 1771) (Orthoptera: Gryllidae):Presence Of Balbiani Body, Özlem Çakici
Turkish Journal of Zoology
Oocyte development in the ovary of the black cricket, Melanogryllus desertus, was investigated. In the panoistic ovary of M. desertus, oocyte developmental stages based on their histological properties were classified as previtellogenic, vitellogenic, and maturation. Oocytes were encircled by follicle cells through the developmental stages. In the previtellogenic stage, a few vacuoles were observed just beneath the oolemma. At the beginning of the vitellogenic stage, subdivided into early-, middle-, and late-vitellogenic stages, yolk granules within the oocyte were initially observed just beneath the oolemma. In addition, small lipid droplets were initially present under the yolk granules. As vitellogenesis proceeded, both …
Tumor Necrosis Factor-Alpha Induced Caspase-3 Activation-Related Inos Gene Expression In Adp-Activated Platelets, Özge Çevi̇k, Zelal Adigüzel, Ahmet Tarik Baykal, Azi̇ze Şener
Tumor Necrosis Factor-Alpha Induced Caspase-3 Activation-Related Inos Gene Expression In Adp-Activated Platelets, Özge Çevi̇k, Zelal Adigüzel, Ahmet Tarik Baykal, Azi̇ze Şener
Turkish Journal of Biology
Platelets are sensitive cells and are easily activated by different stimulants in the circulation system. It is known that tumor necrosis factor-alpha (TNF-α) is a proinflammatory cytokine and plays a role in inflammation. The role of TNF-α in the apoptotic process in blood platelets is unknown. In order to study the formation of apoptosis in platelets after incubation with TNF-α and/or ADP, several biomarkers were chosen: phosphatidylserine (PS) exposure and P-selectin binding; cGMP, Cyt-c, and Ca2+ levels and NOS activation; and gene and protein expression of caspase-3 and iNOS. Platelets were incubated with 100 pg/mL TNF-α and/or 50 mM iNOS …
Combination Of Esomeprazole With Chemotherapeutics Results In More Pronounced Cytotoxic Effect Via Apoptosis On A549 Nonsmall-Cell Lung Cancer Cell Line, Arzu Yilmaztepe Oral, Haluk Barbaros Oral, Mehmet Sarimahmut, Buse Cevatemre, Güven Özkaya, Şeni̇z Korkmaz, Engi̇n Ulukaya
Combination Of Esomeprazole With Chemotherapeutics Results In More Pronounced Cytotoxic Effect Via Apoptosis On A549 Nonsmall-Cell Lung Cancer Cell Line, Arzu Yilmaztepe Oral, Haluk Barbaros Oral, Mehmet Sarimahmut, Buse Cevatemre, Güven Özkaya, Şeni̇z Korkmaz, Engi̇n Ulukaya
Turkish Journal of Biology
The vacuolar (H+)-ATPases that pump H+ from the cytoplasm to extracellular compartments can alter the pH of the tumor microenvironment. Esomeprazole can effectively inhibit vacuolar (H+)-ATPases and may increase the effectiveness of chemotherapeutics. Therefore, we used esomeprazole in combination with cisplatin, carboplatin, paclitaxel, docetaxel, gemcitabine, and vinorelbine on the A549 nonsmall-cell lung cancer cell line. Cisplatin and carboplatin combinations with esomeprazole exhibited superior cytotoxicity compared to the other selected chemotherapeutics. Low-dose combinations of esomeprazole with either cisplatin or carboplatin resulted in synergistic interaction. We examined cytotoxic activity of these combinations with the xCELLigence real-time cytotoxicity assay and detected that esomeprazole …
Microrna-34a Regulates Brain-Derived Neurotrophic Factorin An Intracerebral Hemorrhage Model, Jin Hee Kim, Jae-Sun Choi
Microrna-34a Regulates Brain-Derived Neurotrophic Factorin An Intracerebral Hemorrhage Model, Jin Hee Kim, Jae-Sun Choi
Turkish Journal of Biology
Intracerebral hemorrhages (ICHs) are devastating neurological events frequently resulting in a serious negative prognosis. The exact physiological and disease processes involved in ICHs are complex, but are thought to involve microRNAs (miRNAs), 22 nucleotide small noncoding RNAs that control a variety of normal physiological and disease processes. In this study, we show that a miRNA, miR-34a, regulates BDNF in a model of ICH injury. In particular, we assessed the impact of AM34a, an inhibitor of miR-34a, on the toxicity of thrombin-induced apoptosis and on BDNF-mediated signaling. We investigated the increased expression of miR-34a after an ICH-induced thrombin toxicity injury using …
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Changes In Apoptosis-Related Gene Expression Profiles In Cancer Cell Lines Exposed To Usnic Acid Lichen Secondary Metabolite, Adnan Berk Di̇nçsoy, Demet Cansaran Duman
Turkish Journal of Biology
The presence of uninhibited side effects of cancer drugs often used in cancer treatment has stimulated the search for alternative therapeutic approaches. Therefore, anticarcinogenic effects of synthetic, herbal, and fungal drugs have been investigated for the treatment of various cancer types in recent studies. Lichens, symbiotic organisms of fungi and algae, synthesize metabolites with significant biological activities. The aim of the current study was to screen the anticancer potential of usnic acid on various types of nonmalignant cell lines (Vero, L929) and cancer cell lines (CaCo2, RD, Hep2C, HepG2, Wehi). The growth inhibitory effect of usnic acid was determined by …