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Assessment Of Genetic Diversity And Phylogenetic Relationships Of Endangered Endemic Plant Barbarea Integrifolia Dc. (Brassicaceae) In Turkey, Ertuğrul Fi̇li̇z, Etem Osma, Ali̇ Kandemi̇r, Hüseyi̇n Tombuloğlu, Guzi̇n Tombuloglu, Seda Bi̇rbi̇lener, Mehtap Aydin
Assessment Of Genetic Diversity And Phylogenetic Relationships Of Endangered Endemic Plant Barbarea Integrifolia Dc. (Brassicaceae) In Turkey, Ertuğrul Fi̇li̇z, Etem Osma, Ali̇ Kandemi̇r, Hüseyi̇n Tombuloğlu, Guzi̇n Tombuloglu, Seda Bi̇rbi̇lener, Mehtap Aydin
Turkish Journal of Botany
Barbarea integrifolia DC. (Brassicaceae) is an endangered and endemic species located in Erzincan and Gümüşhane provinces of Turkey. In total, 27 individuals from 2 natural populations were assessed using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) method coupled with sequence analysis of the internal transcribed spacer (ITS1) rDNA region. Genetic accuracy of RAPD-PCR was tested with 25 RAPD primers, and it resulted in 115 clear and reproducible DNA fragments from 13 RAPD primers. Among these, 76 (66.1%) fragments were found to be polymorphic. According to the ITS1 analysis of B. integrifolia populations, the plants exhibited a relatively poor …