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- Cytotoxicity (7)
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- Bacillus thuringiensis (2)
- Biomaterials (2)
- Caspase-3 (2)
- Essential oil (2)
- Expression (2)
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- Laccase (2)
- Maize (2)
- NADPH oxidase (2)
- Osmotic stress (2)
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- Pichia pastoris (2)
- Platelets (2)
- Polyamines (2)
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- Recombinant protein (2)
- Reference gene (2)
- Sweet potato (2)
- Zea mays (2)
- 'omic' disciplines (1)
- 5'-deletion fragments (1)
- 79 AD eruption of Vesuvius (1)
- ADMET analysis (1)
Articles 1 - 30 of 95
Full-Text Articles in Life Sciences
Studies On Organogenesis During Regeneration In The Earthworm, Eudrilus Eugeniae, In Support Of Symbiotic Association With Bacillus Endophyticus, Elaiya Raja Subramanian, Dineshkumar Sudalaimani, Johnson Retnaraj Samuel Selvan Christyraj, Kalidas Ramamoorthy M, Nino Gopi D, Jackson Durairaj Selvan Christyraj, Kasimani Renganathan, Sundar Krishnan, Sudhakar Sivasubramaniam
Studies On Organogenesis During Regeneration In The Earthworm, Eudrilus Eugeniae, In Support Of Symbiotic Association With Bacillus Endophyticus, Elaiya Raja Subramanian, Dineshkumar Sudalaimani, Johnson Retnaraj Samuel Selvan Christyraj, Kalidas Ramamoorthy M, Nino Gopi D, Jackson Durairaj Selvan Christyraj, Kasimani Renganathan, Sundar Krishnan, Sudhakar Sivasubramaniam
Turkish Journal of Biology
Organogenesis and animal adaptation upon loss of body parts are crucial events in regeneration of all traits, and both are uncovered areas in biology. The organogenesis of the mouth and anus during regeneration was studied in Eudrilus eugeniae. It was found that the earthworm regenerates a functional mouth and anus in six days. During the course of organogenesis, the earthworm adopts unique starvation behavior for 5 days, and the starvation triggers the gut microflora of E. eugeniae to produce riboflavin. One of the efficient riboflavin-producing bacteria, Bacillus endophyticus, confirmed through 16S rRNA sequencing, has been isolated from its gut. The …
Cloning And Characterization Of A Serine/Threonine Protein Phosphatase2a-Encoding Gene Ibpp2a1 From Ipomoea Batatas (L.) Lam., Pan Xu, Bin Yong, Huan-Huan Shao, Jiabin Shen, Bin He, Qinqin Ma, Xianghua Yuan, Yu Wang
Cloning And Characterization Of A Serine/Threonine Protein Phosphatase2a-Encoding Gene Ibpp2a1 From Ipomoea Batatas (L.) Lam., Pan Xu, Bin Yong, Huan-Huan Shao, Jiabin Shen, Bin He, Qinqin Ma, Xianghua Yuan, Yu Wang
Turkish Journal of Biology
Sweet potato is one of the most important food crops with strong environmental adaptability. Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that plays crucial roles in hormone signal transduction and abiotic stress response. Characterization of PP2A can elucidate the mechanisms of stress resistance in this crop. In this study, a total of 15 PP2A transcripts, including nine regulatory subunit-encoding sequences and six catalytic subunit-encoding sequences, were identified from sweet potato and found to be expressed at varying levels. Only one contained a complete open reading frame and encoded a B regulatory subunit (termed IbPP2A1). Ten polymorphic sites …
Lipocalin Gene Expression Is Varied In Developmental Stages By Larval Nutritional Stress In Drosophila, Nazli Ayhan, Pinar Güler, Banu Şebnem Önder
Lipocalin Gene Expression Is Varied In Developmental Stages By Larval Nutritional Stress In Drosophila, Nazli Ayhan, Pinar Güler, Banu Şebnem Önder
Turkish Journal of Biology
Lipocalin genes NLaz, GLaz, and Karl are evolutionarily conserved genes in Drosophila melanogaster. There are studies on lipocalin gene expression differences under diverse diet conditions, but these studies have focused mainly on age-dependent expression profiles of these genes. The main aim of our study is to determine lipocalin expression in the developmental period by nutritional manipulation with an isofemale-based design. Three larval developmental periods have been researched under normal and restricted diets. We found significant differences between lines during their developmental time-related lipocalin expression. Here, we demonstrate that upregulations in the early developmental stages of lipocalin genes under stressful conditions …
Cryopreservation Of Citrus Seed Via Dehydration Followedby Immersion In Liquid Nitrogen, Ergun Kaya, Fernanda Souza, Emel Yilmaz Gökdoğan, Muammer Ceylan, Maria Jenderek
Cryopreservation Of Citrus Seed Via Dehydration Followedby Immersion In Liquid Nitrogen, Ergun Kaya, Fernanda Souza, Emel Yilmaz Gökdoğan, Muammer Ceylan, Maria Jenderek
Turkish Journal of Biology
An important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. x Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability …
Population Variation In Drought Resistance And Its Relationship With Adaptiveand Physiological Seedling Traits In Turkish Red Pine (Pinus Brutia Ten.), Gaye Kandemi̇r, Sertaç Önde, Fati̇h Temel, Zeki̇ Kaya
Population Variation In Drought Resistance And Its Relationship With Adaptiveand Physiological Seedling Traits In Turkish Red Pine (Pinus Brutia Ten.), Gaye Kandemi̇r, Sertaç Önde, Fati̇h Temel, Zeki̇ Kaya
Turkish Journal of Biology
Variation in drought resistance and its relationship with adaptive and physiological traits in forest trees are important in choosing suitable seed sources for reforestation and afforestation programs. A common garden experiment using 240 half-sib families originating from coastal and inland populations of Turkish red pine (Pinus brutia) in Turkey was set up with three replicates. The aims were to determine variation of drought damage, height growth, and phenology among populations and to investigate the relationship between drought damage and physiological traits (i.e. plant moisture stress and proline content). Three-year-old seedlings were subjected to drought treatment during the summer of 2000 …
Heterologous Expression And Characterization Of A High Redox Potential Laccase Fromcoriolopsis Polyzona Mucl 38443, Orkun Pi̇nar, Candan Tamerler Behar, Ayten Karataş
Heterologous Expression And Characterization Of A High Redox Potential Laccase Fromcoriolopsis Polyzona Mucl 38443, Orkun Pi̇nar, Candan Tamerler Behar, Ayten Karataş
Turkish Journal of Biology
In this study, a novel laccase gene, named as Cplcc1, and its corresponding cDNA were isolated and characterized from the Coriolopsis polyzona MUCL 38443 strain. The Cplcc1 gene consists of a 1563-bp open reading frame encoding a protein of 520 amino acids with a 20-residue putative signal peptide. The size of the Cplcc1 gene is 2106 bp and it contains ten introns and five potential N-glycosylation sites. Additionally, the isolated full-length Cplcc1 cDNA was successfully expressed in Pichia pastoris. The heterologous expression conditions were also optimized and the highest activity value increased to 800 U L-1 with 1.5% methanol, 0.8 …
Exogenously Sourced γ-Irradiated Chitosan-Mediated Regulation Of Growth, Physiology, Quality Attributes And Yield In Mentha Piperita L., Bilal Ahmad, Mohammad Masroor Akhtar Khan, Hassan Jaleel, Yawar Sadiq, Asfia Shabbir, Moin Uddin
Exogenously Sourced γ-Irradiated Chitosan-Mediated Regulation Of Growth, Physiology, Quality Attributes And Yield In Mentha Piperita L., Bilal Ahmad, Mohammad Masroor Akhtar Khan, Hassan Jaleel, Yawar Sadiq, Asfia Shabbir, Moin Uddin
Turkish Journal of Biology
In view of the increasing demand for essential oils from medicinal and aromatic plants, the application of irradiated polysaccharides like chitosan is a promising option. A pot experiment was performed to study the influence of various concentrations of irradiated chitosan (ICN) on growth, physiological, and yield parameters of Mentha piperita L., a plant with considerable pharmaceutical importance. The treatments were foliar spray of deionized water only (control), foliar spray of unirradiated chitosan (40 mg L-1), and ICN at 40, 80, 120, and 160 mg L-1. Among these, 80 mg L-1 ICN (treatment 4) proved to be the best for most …
Histomorphometric Evaluation Of The Testicular Parenchyma Of Rats Submittedto Protein Restriction During Intrauterine And Postnatal Life, Jessica Oliveira, Alluanan Silva, Sandra Souza, Rosana Morais, Elizabeth Neves Melo, Frederico Maia, Valdemiro Silva Junior
Histomorphometric Evaluation Of The Testicular Parenchyma Of Rats Submittedto Protein Restriction During Intrauterine And Postnatal Life, Jessica Oliveira, Alluanan Silva, Sandra Souza, Rosana Morais, Elizabeth Neves Melo, Frederico Maia, Valdemiro Silva Junior
Turkish Journal of Biology
The critical period of development is highly susceptible to disorders. Environmental contaminants, stress, and poor nutrition may permanently affect structurally and functionally an organism during adulthood. Protein restriction in intrauterine and neonatal periods may impair testicular cells and reduce steroidogenic activity. The current study investigated the effect of low protein diet during intrauterine and postnatal life on testicular function in immature and adult rats. Pregnant Wistar rats were fed either a normal protein diet or a low protein diet during pregnancy until birth and during lactation until weaning. The results showed that undernutrition during intrauterine life reduced epithelium height, tubular …
Identification And In Silico Analysis Of Galns Mutations Causing Morquio A Syndrome In Eight Consanguineous Families, Irfan Ullah, Abdul Nasir, Sarmad Mehmood, Sohail Ahmed, Muhammad Ikram Ullah, Asmat Ullah, Abdul Aziz, Syed Irfan Raza, Khadim Shah, Saad Ullah Khan, Muhammad Jawad Hassan, Wasim Ahmad
Identification And In Silico Analysis Of Galns Mutations Causing Morquio A Syndrome In Eight Consanguineous Families, Irfan Ullah, Abdul Nasir, Sarmad Mehmood, Sohail Ahmed, Muhammad Ikram Ullah, Asmat Ullah, Abdul Aziz, Syed Irfan Raza, Khadim Shah, Saad Ullah Khan, Muhammad Jawad Hassan, Wasim Ahmad
Turkish Journal of Biology
Genetic deficiency of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) due to mutations in the GALNS gene results in the pathogenesis of Morquio A syndrome. To date, more than 200 mutations have been reported in GALNS, resulting in variable clinical features. For this reason, bioinformatics analysis of these mutations is important to determine their effect on the structure and functions of the protein and to establish a correlation between genotype and phenotype. In the present study, eight Pakistani consanguineous families with Morquio A syndrome were clinically and genetically evaluated. Linkage analysis followed by sequence analysis of the gene detected four novel (p.Phe216Ser, p.Met38Arg, p.Ala291Ser, …
Cell-Compatible Phb/Silk Fibroin Composite Nanofibermat For Tissue Engineering Applications, Zeynep Karahali̇loğlu
Cell-Compatible Phb/Silk Fibroin Composite Nanofibermat For Tissue Engineering Applications, Zeynep Karahali̇loğlu
Turkish Journal of Biology
Cell compatibility is one of the prominent requirements for the fabrication of tissue engineering materials. Silk fibroin (SF) is an excellent material for biomedical applications and shows desirable properties such as good compatibility, minimal tissue reaction, and tailorable degradability. Poly[(R)-3-hydroxybutyrate] (PHB) has a high percentage of crystallinity and a high melting temperature. In this study, PHB and SF were blended together to improve the mechanical properties of the SF fibrous structure and the crystallinity of PHB. Furthermore, the cell-biomaterial interaction on the PHB/SF composite scaffold was expected to be enhanced via the SF. For this purpose, PHB/SF composite scaffolds were …
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
An In Vitro Human Skeletal Muscle Model: Coculture Of Myotubes,Neuron-Like Cells, And The Capillary Network, Ayşe Burcu Ertan, Hali̇me Kenar, Tahsi̇n Beyza Beyzadeoğlu, Fatma Neşe Kök, Gamze Köse
Turkish Journal of Biology
This study reports the generation of a new human muscle tissue equivalent from skeletal muscle-derived stem cells and human umbilical vein endothelial cells (HUVECs). Skeletal muscle stem cells were isolated by the preplate technique and differentiated into neuron-like cells that were positive for neuronal beta-tubulin3 and nestin and negative for the astrocyte marker glial fibrillary acidic protein (GFAP). Coculture of skeletal muscle stem cells with the HUVECs under optimized fetal bovine serum and media conditions resulted in formation of a capillary network among the multinucleated myotubes. The neuron-like cells derived from the human skeletal muscle stem cells were seeded onto …
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
The Jasmonate-Zim Domain Protein Ftjaz2 Interacts With The R2r3-Mybtranscription Factor Ftmyb3 To Affect Anthocyanin Biosynthesis In Tartary Buckwheat, Xiao-Peng Luo, Shuang-Jiang Li, Pan-Feng Yao, Cheng-Lei Li, Hui Chen, Qi Wu, Hai-Xia Zhao
Turkish Journal of Biology
Tartary buckwheat is a type of coarse cereal that contains a rich supply of flavonoids. When suffering from certain stresses, tartary buckwheat can respond by promoting the biosynthesis of anthocyanins. It is well established that the jasmonate (JA) signaling pathway plays a critical role in plant resistance to disease by stimulating the production of anthocyanins; furthermore, the genes encoding the jasmonate-ZIM domain (JAZ) proteins have been shown to be key players in this process. However, the role of JAZ proteins in tartary buckwheat is poorly understood. In this study, we aimed to isolate FtJAZs from tartary buckwheat and identify FtJAZs …
Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler
Exogenously Applied Polyamines Ameliorate Osmotic Stress-Induced Damages And Delay Leaf Rolling By Improving The Antioxidant System In Maize Genotypes, Ni̇hal Çalişkan, Asi̇m Kadioğlu, Nesli̇han Saruhan Güler
Turkish Journal of Biology
Leaf rolling (LR), particularly in crops, is a dehydration avoidance mechanism that results from water loss in plants under drought conditions. Two maize cultivars (Zea mays L.) differing in their tolerance to drought (cultivar Side was selected as stress-tolerant and cultivar Karaçay was selected as stress-sensitive) under PEG-induced drought stress were used in this study to understand the effects of polyamines (PAs) on LR response and the role of the antioxidant system in the delayed LR process. Aerial parts of the seedlings were excised prior to the experiment and then submerged in a Hoagland nutrient solution with or without 0.1 …
The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada
The Effect Of Various Inducers And Their Combinations With Copper On Laccaseproduction Of Trametes Versicolor Pellets In A Repeated-Batch Process, Emre Bi̇rhanli, Özfer Yeşi̇lada
Turkish Journal of Biology
The aim of this study was to increase laccase production in Trametes versicolor ATCC 200801 pellets by using various inducers and their combinations under repeated-batch conditions. Because copper is an effective inducer for laccase production, the effect of Cu on laccase production in this strain was tested first. The optimal Cu concentration for the highest laccase production was 0.5 mM. Following this preliminary study, the effect of other inducers [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), syringaldazine, guaiacol, and 2,5-xylidine] on laccase production was determined. Copper was determined to be the most efficient inducer among the inducers used. Therefore, the synergistic effect of each …
Recovery And Amplification Of Ancient Dna From Herculaneum Victims Killed By The 79 Ad Vesuvius Hot Surges, Fabi̇o M. Guari̇no, Claudio Buccelli, Vincenzo Graziano, Pietro La Porta, Marcello Mezzasalma, Gaetano Odierna, Mariano Paternoster, Pierpaolo Petrone
Recovery And Amplification Of Ancient Dna From Herculaneum Victims Killed By The 79 Ad Vesuvius Hot Surges, Fabi̇o M. Guari̇no, Claudio Buccelli, Vincenzo Graziano, Pietro La Porta, Marcello Mezzasalma, Gaetano Odierna, Mariano Paternoster, Pierpaolo Petrone
Turkish Journal of Biology
n thermally damaged archaeological bones the quantity, quality, and amplifiability of DNA are very much reliant on both the extent of heating and the environmental conditions of the burial context. In this study we tested the possibility of extracting and amplifying ancient DNA from human bone remains of Herculaneum victims of the 79 AD eruption of Vesuvius using a combination of histochemical and molecular methods. Long bone samples with variable degrees of chromatic and morphological alterations consistent with exposure to temperatures of about 300 °C were taken from four specimens. Using histochemical stains, bone cryostat sections from three individuals revealed …
Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu
Cloning, Expression, And Activity Analysis Of Human Cathepsin C In The Yeast Pichia Pastoris, Cenk Dağlioğlu
Turkish Journal of Biology
The yeast Pichia pastorisexpression system was investigated for the production of human cathepsin C (CatC) recombinant protein. The full-length CatC cDNA, corresponding to amino acids 12-475, was synthesized from interleukin-2 (IL-2) stimulated human peripheral blood mononuclear cells and subcloned in the pGEM-T cloning vector. After confirming the DNA sequence of the insert, the gene was cloned into the pPICZ\alphaA expression vector under the control of the methanol-inducible alcohol oxidase (AOX1) promoter and transformed to P. pastoris X-33 cells. The expressed protein was secreted into the culture medium through the alpha-factor mating signal sequence of the expression vector. Analysis of the …
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Interaction Of Adipose-Derived Mesenchymal Stem Cells With Mcf-7 Cells In Vitro:A Study Emphasizing Signaling Molecule Expression And Transcriptional Changes, Qiong Wu, Chang-Xin Jin, Hui Chen, Xue-Yong Li, Yuejin Li
Turkish Journal of Biology
Adipose-derived stem cells (ADSCs) promote metastasis of breast cancer cells that can differentiate into carcinoma-associated cells in tumor microenvironments. However, the precise mechanism is poorly understood. This study shows that interaction of ADSCs with breast cancer MCF-7 cells changes the level of metastasis-related functional proteins in MCF-7 cells, as well as that of oncogenes in ADSCs. ADSCs were isolated from adipose tissues of patients. The interaction of ADSCs with MCF-7 cells was performed by coculturing ADSCs (or MCF-7 cells) with exosomes derived from MCF-7 cells (or ADSCs). Exosomes were labeled by DiI. In cocultures, migration-related regulators in MCF-7 cells were …
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
N-Acetylglucoseamine Modified Alginate Sponges As Scaffolds For Skin Tissue Engineering, Murat Demi̇rbi̇lek, Neli̇sa Türkoğlu, Selçuk Aktürk
Turkish Journal of Biology
In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and …
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Role Of Alternative Phosphorylation And O-Glycosylation Of Erythropoietinreceptor In Modulating Its Function: An In Silico Study, Humera Kausar, Sana Gul, Waqar Ahmad, Sana Awan, Muhammad Tahir Sarwar, Bushra Ijaz, Muhammad Ansar, Sultan Asad, Sajida Hassan
Turkish Journal of Biology
Detailed knowledge of the three-dimensional (3D) structure of a protein is essential for the proper understanding of its function(s) that could be modified through posttranslational modifications (PTMs). Among these PTMs, alterations of serine/threonine residues of a protein through phosphorylation and O-glycosylation are extremely dynamic and could modulate the functions of a protein by affecting their 3D structure. Potential of a protein for certain PTMs could be evaluated through computer-based methods. Erythropoietin (EPO) is a multifunctional protein that primarily regulates red blood cell production and is also involved in other nonhematopoietic functions; for example, EPO also has cardioprotective and neuroprotective effects. …
Production And Characterization Of Polyclonal Antibodies To Human Recombinant Domain B-Free Antihemophilic Factor Viii, Artem Tykhomyrov, Victor Nedzvetsky, Sergiy Shemet, Can Ali̇ Ağca
Production And Characterization Of Polyclonal Antibodies To Human Recombinant Domain B-Free Antihemophilic Factor Viii, Artem Tykhomyrov, Victor Nedzvetsky, Sergiy Shemet, Can Ali̇ Ağca
Turkish Journal of Biology
Moroctocog alpha is a human B-domain deleted recombinant factor VIII (BDDrFVIII), which represents a new generation of pure antihemophilic products. We describe here an optimized procedure for polyclonal anti-FVIII-antibody production with the use of BDDrFVIII as an immunogen. The main immunochemical characteristics of the produced antibodies and their potential biomedical applications are also reported. Rabbits were immunized with BDDrFVIII as an emulsion with Freund's adjuvant or with antigen immobilized in polyacrylamide gel (PAAG). Antibody titers in immune sera were assayed by enzyme-linked immunosorbent assay (ELISA). IgG purification was performed by affine chromatography on protein A-sepharose. Immune sera and IgG were …
Mg Deficiency Changes The Isoenzyme Pattern Of Reactive Oxygen Species-Relatedenzymes And Regulates Nadph-Oxidase-Mediated Ros Signaling In Cotton, Rengi̇n Özgür Uzi̇lday, Bariş Uzi̇lday, Tolga Yalçinkaya, İsmai̇l Türkan
Mg Deficiency Changes The Isoenzyme Pattern Of Reactive Oxygen Species-Relatedenzymes And Regulates Nadph-Oxidase-Mediated Ros Signaling In Cotton, Rengi̇n Özgür Uzi̇lday, Bariş Uzi̇lday, Tolga Yalçinkaya, İsmai̇l Türkan
Turkish Journal of Biology
The aim of this work was to investigate changes in isoenzyme patterns of enzymes related to reactive oxygen species (ROS) detoxification such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR), and glutathione-S-transferase (GST) in cotton under Mg deficiency. Moreover, we aimed to elucidate how a ROS producer, NADPH oxidase (NOX), responds to changing Mg levels. Cotton plants were grown with different concentrations of MgSO4 in hydroponic medium to create nutrient deficiency (0, 75, 150, 1000 μM Mg). Gradual decreases in growth and photosynthetic rates were observed with declining Mg concentrations and 0, 75, and …
Melatonin Diminishes Oxidative Damage In Sperm Cells,Improving Assisted Reproductive Techniques, Fabián Monllor, Javier Espino, Ana María Marchena, Águeda Ortiz, Graciela Lozano, Juan Francisco García, Jose A. Pariente, Ana B. Rodriguez, Ignacio Bejarano
Melatonin Diminishes Oxidative Damage In Sperm Cells,Improving Assisted Reproductive Techniques, Fabián Monllor, Javier Espino, Ana María Marchena, Águeda Ortiz, Graciela Lozano, Juan Francisco García, Jose A. Pariente, Ana B. Rodriguez, Ignacio Bejarano
Turkish Journal of Biology
Sperm preparation procedures are a potential generator of oxidative stress-induced DNA damage, which leads to a dramatic drop in fertility. An increasing number of studies suggest that melatonin reduces the oxidative stress induced by manipulation. However, very little is known about the preservative role of melatonin in sperm preparation medium during assisted reproduction procedures. For this aim to be achieved, semen was divided into two fractions and preincubated with and without 1 mM melatonin. Afterwards, both fractions were divided into two subfractions to perform swim-up in the presence and absence of 1 mM melatonin. Labeling with anti-CD46 and antiactive caspase-3 …
Exploration Of The Binding Pocket Of Histone Deacetylases: The Design Of Potent And Isoform-Selective Inhibitors, Abdullahi̇ İbrahi̇m Uba, Kemal Yelekçi̇
Exploration Of The Binding Pocket Of Histone Deacetylases: The Design Of Potent And Isoform-Selective Inhibitors, Abdullahi̇ İbrahi̇m Uba, Kemal Yelekçi̇
Turkish Journal of Biology
Histone deacetylases (HDACs) are enzymes that act on histone proteins to remove the acetyl group and thereby regulate the chromatin state. HDACs act not only on histone protein but also nonhistone proteins that are key players in cellular processes such as the cell cycle, signal transduction, apoptosis, and more. "Classical" HDACs have been shown to be promising targets for anticancer drug design and development. However, the selectivity of HDAC inhibitors for HDAC isoforms remains the motivation of current research in this field. Here, we explored Class I HDACs and HDAC6 by sequence alignment and structural superimposition, catalytic channel extraction, and …
Synergistic Effect Of 24-Epibrassinolide And Salicylic Acid On Photosynthetic Efficiency And Gene Expression In Brassica Juncea L. Under Pb Stress, Sukhmeen Kaur Kohli, Neha Handa, Anket Sharma, Vinod Kumar, Parminder Kaur, Renu Bhardwaj
Synergistic Effect Of 24-Epibrassinolide And Salicylic Acid On Photosynthetic Efficiency And Gene Expression In Brassica Juncea L. Under Pb Stress, Sukhmeen Kaur Kohli, Neha Handa, Anket Sharma, Vinod Kumar, Parminder Kaur, Renu Bhardwaj
Turkish Journal of Biology
Lead (Pb) has wide-ranging effects on various essential physiological processes in plants including seed germination, root/shoot growth, photosynthetic efficiency, water status, and activities of enzymes. The effect of combined treatment of 24-epibrassinolide (10-7 M, EBL) and salicylic acid (1 mM, SA) on growth, photosynthetic attributes, and phenolic compounds in 30-, 60-, and 90-day-old plants of Brassica juncea L. under Pb stress (0.25 mM, 0.50 mM, and 0.75 mM) were studied. It was observed that Pb toxicity resulted in lowered growth and photosynthetic efficiency. The expressions of CHLASE, PSY, CHS, and PAL genes were altered. Presoaking treatment with the combination of …
Stably Expressed Reference Genes During Differentiation Ofbone Marrow-Derived Mesenchymal Stromal Cells, Ilgin Cagnan, Fatima Aerts Kaya, Fahri̇ye Duygu Çeti̇nkaya, Ayşen Özcan
Stably Expressed Reference Genes During Differentiation Ofbone Marrow-Derived Mesenchymal Stromal Cells, Ilgin Cagnan, Fatima Aerts Kaya, Fahri̇ye Duygu Çeti̇nkaya, Ayşen Özcan
Turkish Journal of Biology
Human mesenchymal stromal cells (MSCs) from different parts of the body (i.e. bone marrow, BM) have distinct cellular and molecular features including global gene expression profiles. Quantitative polymerase chain reaction is a reliable method used in the quantification of gene expression. Correct assessment of target gene expression mostly depends on the reference gene (RG) of choice. Herein, expression levels of RGs (n = 19) in adipo-/osteogenic-differentiated and control (uninduced) BM-MSCs from donors were measured using the RealTime ready Human Reference Gene Panel. Characterization of BM-MSCs was assessed using in vitro differentiation by histochemical staining and immunophenotyping of cells by flow …
Amperometric Biosensor For Detection Of Triglyceride Tributyrinbased On Zero Point Charge Of Activated Carbon, Mohsen Rezvani, Ghasem Najafpour, Maedeh Mohammadi, Hossein Zare
Amperometric Biosensor For Detection Of Triglyceride Tributyrinbased On Zero Point Charge Of Activated Carbon, Mohsen Rezvani, Ghasem Najafpour, Maedeh Mohammadi, Hossein Zare
Turkish Journal of Biology
A simple and robust single enzyme biosensor was fabricated for triglyceride (TG) detection. Graphite rods were modified with activated carbon (AC) and used as support for lipase immobilization. Chitosan (CHIT) was eventually used to create film on the bioelectrode and retain the immobilized enzyme. To extend the linear range of TG detection, AC was functionalized with carboxyl and then amine groups (AAC) to enhance the isoelectric point of AC. The constructed graphite/AAC/lipase /CHIT bioelectrode was characterized using cyclic voltammetry and field emission scanning electron microscopy (FE-SEM). The accuracy of the developed biosensor was assessed through determination of different concentrations of …
Optimization, Purification, And Characterization Of An Extracellular Antifungal Protein From Serratia Marcescens Dt3 Isolated From Soil In Vietnam, Tuyen Thi Do, Thao Thi Nguyen, Thanh Sy Le Nguyen, Hoang Thanh Le
Optimization, Purification, And Characterization Of An Extracellular Antifungal Protein From Serratia Marcescens Dt3 Isolated From Soil In Vietnam, Tuyen Thi Do, Thao Thi Nguyen, Thanh Sy Le Nguyen, Hoang Thanh Le
Turkish Journal of Biology
Gram-negative Serratia marcescens is a potential biological control agent of fungal plant diseases in many tropical regions. Its antifungal activity is due to the production of prodigiosin, protease, chitinase, and other substances that act to weaken the fungal cell wall and cause lysis. In the following investigation, a novel extracellular protein with antifungal activity against Rhizoctonia solani and Fusarium oxysporum was optimally produced, purified, and characterized from a native Serratia marcescens DT3. Under optimal conditions (medium containing 1.25% peptone, 0.5% yeast extract; temperature of 28 °C; initial pH of 7; culture time of 28 h), the antifungal activity of the …
A R2r3 Myb Transcription Factor From Ash Positively Regulates Salt Response In Tobacco, Tian Li, Jingkuan Sun, Yuping Bi
A R2r3 Myb Transcription Factor From Ash Positively Regulates Salt Response In Tobacco, Tian Li, Jingkuan Sun, Yuping Bi
Turkish Journal of Biology
The roles of MYB transcription factors are diverse and important in regulating environmental stress in plants. In this study, the R2R3 MYB gene FvMYB2 (GenBank Accession No. KY767843) and its promoter region were cloned from ash (Fraxinus velutina Torr.). Phylogenetic analysis revealed that FvMYB2 was clustered with VvMYB60 from Vitis vinifera. PlantCARE software analysis showed that its promoter contained different cis elements involved in diverse abiotic stresses. The expression patterns of FvMYB2 were investigated under different abiotic stress conditions. FvMYB2 subcellular localization was mainly localized in the nucleus. Overexpression of FvMYB2 in tobacco showed enhanced salt resistance and upregulation of …
Effect Of Nutrient Media And Initial Biomass On Growth Rate And Nutrient Uptake Of Sargassum Spinuligerum (Sargassaceae, Phaeophyta), Tin Cong Hoang, Ravi Fotedar, Michael O'Leary
Effect Of Nutrient Media And Initial Biomass On Growth Rate And Nutrient Uptake Of Sargassum Spinuligerum (Sargassaceae, Phaeophyta), Tin Cong Hoang, Ravi Fotedar, Michael O'Leary
Turkish Journal of Biology
The Sargassum species are prospective candidates for marine culture, but there are a limited number of reports on their nutrient requirements and optimum initial stocking biomass, and nothing is published for Sargassum spinuligerum. This study investigated the effects of three commercially available fertilizers (Hortico, Seasol, and Aquasol) and four initial stocking biomass levels of S. spinuligerum on the growth rate and nutrient uptake capacities for 7 weeks. The results showed that S. spinuligerum could be grown under outdoor conditions with the optimum initial stocking biomass of 15.35 g per 113 L. The different commercial fertilizers significantly influenced the specific growth …
Screening Of Yeast Isolates From Flowers For Effective Ethanol Production, Asma Chaudhary, Shuichi Karita
Screening Of Yeast Isolates From Flowers For Effective Ethanol Production, Asma Chaudhary, Shuichi Karita
Turkish Journal of Biology
The use of alternative substrates to produce biofuel is a striking option nowadays. This study aimed to screen bioethanol-producing yeast strains. From different flowers, 65 yeasts were isolated. Twelve isolates assimilated glucose by liberation of CO2 anaerobically. Out of these, only 5 yeast isolates fermented glucose in medium consisting of 0.8 g/L Mg2+ ions to produce 2.05 $\pm$ 0.03% ethanol. The selected five strains were identified as members of the genera Metschnikowia or Meyerozyma based on molecular characterization. Selected yeast strains were used for conversion of rice into bioethanol following dilute acid hydrolysis and fermentation. Consistent ethanol production was 1.80 …