Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Biochemistry, Biophysics, and Structural Biology (2)
- Structural Biology (2)
- Biology (1)
- Biophysics (1)
- Biotechnology (1)
-
- Cellular and Molecular Physiology (1)
- Disease Modeling (1)
- Diseases (1)
- Ecology and Evolutionary Biology (1)
- Environmental Microbiology and Microbial Ecology (1)
- Medicine and Health Sciences (1)
- Microbiology (1)
- Molecular Biology (1)
- Nervous System Diseases (1)
- Physiology (1)
- Skin and Connective Tissue Diseases (1)
- Terrestrial and Aquatic Ecology (1)
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Incidence Of Antibiotic Resistance And Plasmid Content In Freshwater Beach Sand And Water And Clinical Urinary Tract Infection Escherichia Coli Isolates, Robert F. White
Incidence Of Antibiotic Resistance And Plasmid Content In Freshwater Beach Sand And Water And Clinical Urinary Tract Infection Escherichia Coli Isolates, Robert F. White
Biology Theses
Antibiotic-resistant (AR) bacteria have been found in environmental ecosystems including beach sand and water, and pose a serious threat to the mitigation of human and animal disease. The presence of antibiotic residues in the environment, fueled by wastewater effluent and agricultural runoff, may produce selective pressure on introduced microbes such as Escherichia coli, leading to the production of AR populations. This study characterized and compared the antibiotic resistance patterns and plasmid content of E. coli isolated from a freshwater beach and clinical urinary tract infection (UTI) samples. A higher level of antibiotic resistance was expected in clinical (UTI) Escherichia coli …
Life History And Metabolic Responses Of Daphnia Magna Exposed To Effluents Of Urban Origin Treated By Advanced Oxidation, Benjamin Z. Szczygiel
Life History And Metabolic Responses Of Daphnia Magna Exposed To Effluents Of Urban Origin Treated By Advanced Oxidation, Benjamin Z. Szczygiel
Biology Theses
Modern sewage treatment processes do not remove many chemicals that pass through the sewer system, including pharmaceuticals and personal care products that have been shown to have negative effects in ecosystems. New processes have been developed to remove these chemicals, known as advanced oxidation processes (AOPs). In this study, Daphnia magna are used to examine the effects of wastewater effluent treated conventionally compared to effluent treated with AOPs (Peracetic Acid/Ultraviolet Light and Hydrogen Peroxide/Ultraviolet Light). For this purpose, two sets of experiments were preformed: a five-day survivorship trial, and a 14-day life history experiment. Growth, survival, and reproduction of Daphnia …
Cloning And Expression Of Hydra Innexin 2, A Gap Junction Protein Required For Coordinated Contraction Of The Body Column, Ashley O'Brien
Cloning And Expression Of Hydra Innexin 2, A Gap Junction Protein Required For Coordinated Contraction Of The Body Column, Ashley O'Brien
Biology Theses
In invertebrates gap junctions are formed by the innexin family of proteins. Remarkably, the genome of Hydra magnipapillata contains 17 innexin genes. This study focused on Hydra innexin-2 (h-Inx2) which is expressed in nerve cells and plays a role in contraction of the body column. The gene sequence of H-Inx2 was obtained from the National Center for Biotechnology Information (NCBI), the gene was synthesized externally and transferred to a vector suitable for expression in Xenopus oocytes (pcDNA3.1 CT-GFP TOPO). The TOPO CT-GFP vector includes a priming site for RNA polymerase which allows in vitro preparation of RNA. Another advantage is …
Functional Analysis Of A Critical Glycine (Glycine 12) In Beta-Type Connexins Of Human Skin, Rasheed Bailey
Functional Analysis Of A Critical Glycine (Glycine 12) In Beta-Type Connexins Of Human Skin, Rasheed Bailey
Biology Theses
At least five beta-type connexins are expressed in various layers of the skin (Cx26, Cx30, Cx30.3, Cx31, and Cx32) and all include a glycine residue at position 12. Glycine12 (G12) is located about halfway through the cytoplasmic amino terminus and has been the focus of several studies related to connexin diseases and gap junction channel structure. The importance of this residue is evident in the severity and diversity of diseases associated with amino acid substitutions at G12 including hereditary forms of skin disease, deafness and neuropathy. This study uses bioinformatic analysis in combination with mutational analysis and electrophysiology to better …